Biomedical subjects

A G Pockley

Publications and source records attributed to A G Pockley.

74 records · Page 5Linked to original sources

PubMedDate not supplied

The effects of interleukins 5 and 6 on immunoglobulin production in rat lacrimal glands.

This study investigated the effects of recombinant murine IL-5 (rmIL-5) and recombinant human IL-6 (rhIL-6) on immunoglobulin production by cultured rat lacrimal gland tissue, and compared the responses to those of cells of nonmucosal origin. Both rmIL-5 and rhIL-6 enhanced IgA, IgG, and IgM production by resting unfractionated rat spleen cells. Detectable levels of all three immunoglobulin isotypes were observed in the culture supernatants of unstimulated rat lacrimal gland tissue after 7 days in culture. Incubation of lacrimal gland tissue with rmIL-5 (50 U/ml) enhanced the levels of IgA, but had no effect on the levels of IgG or IgM. Incubation with rhIL-6 (50 U/ml) enhanced IgA production, but inhibited the secretion of both IgG and IgM. A combination of both IL-5 and IL-6 enhanced IgA levels above those which were observed for each individual lymphokine and inhibited the production of IgG and IgM to a greater degree than did IL-6 alone. These data support a potential regulatory function for lymphokines in glandular mucosal tissue and suggest the presence of a distinct regulatory mechanism in one glandular mucosal tissue which has not been noted using isolated cells derived from either Peyer's patch or spleen.

Analysis of Variance↗

PubMedDate not supplied

Effect of combined heat, ozonation and ultraviolet irradiation (VasoCare) on heat shock protein expression by peripheral blood leukocyte populations.

The re-administration of whole blood subjected to heat, ozonation and ultraviolet irradiation (VasoCare therapy) has been shown to elicit clinical benefits in individuals with vascular disease. Given that these stressors induce heat shock protein (Hsp) expression and that heat shock protein reactivity is implicated in the pathogenesis of vascular disease, this study assessed the effect of VasoCare on intracellular expression of Hsp60 and Hsp70 by treated peripheral blood leukocytes. Contrary to expectations, VasoCare induced a significant reduction (approximately 40%) in the proportion of peripheral blood mononuclear cells expressing intracellular Hsp60 and Hsp70, whereas it had no effect on heat shock protein expression by peripheral blood neutrophils. Cell surface heat shock protein expression was not detectable. The reduced expression of Hsp60 by mononuclear cells was concomitant with an increase in the levels of Hsp60 in treated plasma. Although the mechanism underlying the clinical effectiveness of VasoCare therapy has yet to be established, it may be that re-administration of treated blood or soluble factors derived therefrom modifies in vivo immune responsiveness to heat shock proteins or associated molecules.

Flow Cytometry↗