Length of oestrus and timing of the LH surge was measured in six British White does, housed with a vasectomized buck (experiment 1). The following breeding season, pulsatile LH release during the cycle was measured in eight does and the pattern of behavioural and vaginal traits in relation to onset of oestrus (time 0) determined (experiment 2). In experiment 1, the interval to first oestrus after introduction of the buck on 1st October was 10.3 +/- 3.0 days (n = 6) but in experiment 2, when the buck was put in on 1st September, first oestrus occurred after 39.3 +/- 3.4 days in 7/8 does and 7 days in 1/8 does indicating that adequate exposure to short days is needed before the buck can initiate ovarian activity. LH pulse frequency increased from 0-1 pulses/8 h to 3 pulses/8 h after luteolysis, with no change in pulse amplitude, suggesting that progesterone regulates LH pulse frequency. Mean LH values rose from basal to 102.1 +/- 7.8 ng/ml, 12 +/- 1.5 h after the onset of oestrus, which was 16.3 +/- 1.7 h in length. Does sought out the buck and displayed tail wagging, bleating and restlessness from -60 h to +36 h relative to the onset of oestrus (time 0). The incidence of these activities rose at -12 h and peaked at 0 h. Tail wagging, but not bleating or restlessness, also increased in intensity at 0 h, as did the intensity with which the doe actively sought out the buck. Vulval redness and swelling and onset of a clear thin vaginal discharge were first observed 1-2 days before oestrus, becoming maximal on day 0. It was concluded that onset of frequent tail wagging was the most useful trait for detecting onset of oestrus.

Animals↗

PubMedDate not supplied

Trypanosoma equiperdum: detection of trypanosomal antibodies and antigen by enzyme-linked immunosorbent assay.

Explore the source record for details and available documents.

Animals↗

PubMedDate not supplied

All metacyclic variable antigen types of Trypanosoma congolense identified using monoclonal antibodies.

Vaccination against the tsetse-borne trypanosomiases has proved impossible because of the trypanosome's ability to generate a seemingly inexhaustible number of variable antigen types in the blood or tissues of the host. Each variable antigen is a glycoprotein which forms a surface coat on the trypanosome and each glycoprotein is the product of a single gene. The full repertoire of such antigens has not been identified for any trypanosome serodeme (genotype) as yet, but the number of genes coding for variable antigen glycoproteins is estimated to be between 100 and 1,000. We have previously postulated that for Trypanosoma brucei the antigen repertoire of the infective metacyclic stage trypanosomes inoculated by the tsetse fly may be considerably smaller than that expressed in the mammalian host. If this is so then protection against infection by the vector becomes an easier proposition, but the actual scale of the metacyclic repertoire is also unknown. We present here evidence that the metacyclic repertoire of a stock of T. congolense, the most important of the pathogenic cattle trypanosomes, is limited to 12 variable antigen types.

Animals↗