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Biomedical subjects

A Funakoshi

Publications and source records attributed to A Funakoshi.

267 records · Page 15Linked to original sources
PubMed1977

Purification and properties of human pancreatic deoxyribonuclease I.

Human pancreatic DNase I was purified extensively from duodenal juice of healthy subjects by a procedure including ammonium sulfate fractionation, ethanol fractionation, phosphocellulose fractionation, isoelectric focusing, and gel filtration. The final preparation was free of DNase II, pancreatic RNase, alkaline phosphatase, and protease. The enzyme had a molecular weight of approximately 30,000, as determined by gel filtration on Sephadex G-100, and showed maximum activity at pH 7.2-7.6. It required divalent cations for activity, and caused single-strand breaks by endonucleolytic attack on double- as well as single-stranded DNA molecules. The enzyme was inhibited by actin and bovine pancreatic DNase I antibody.

Deoxyribonucleases↗
PubMedDate not supplied

Manifestations of experimental acute pancreatitis in young and old rats.

Two kinds of experimental pancreatitis were induced in young (4-6 month) and old (25-27 month) female Wistar rats: acute edematous pancreatitis was induced by intraperitoneal administration of a high dose of cerulein (40 micro/kg x 2) and acute hemorrhagic pancreatitis was intraductal injection of 1% deoxycholic acid. After these treatments, the plasma amylase concentration and pancreatic wet weight were determined and the pancreas was examined histologically. In the groups with cerulein induced pancreatitis one of eight old rats died, whereas all five young rats survived. There was no specific finding macroscopically in the liver, kidney, lung or heart of old rats at autopsy after cerulein injection. The plasma amylase concentration and the pancreatic wet weight were significantly increased by administration of cerulein or deoxycholic acid in both young and old rats. There was no significant difference in the plasma amylase concentrations in young and old rats after the induction of acute pancreatitis. The increase in pancreatic wet weight was less in old rats than in young ones after deoxycholic acid treatment, but similar in the two groups after cerulein injection. The extents of histological changes were also similar in young and old rats. Thus, no evidence that aging increases susceptibility to pancreatitis was obtained.

Journal Article↗
PubMedDate not supplied

Different response of brain cholecystokinin content to intragastric administration of trypsin inhibitor (camostate) in young and old rats.

The tissue concentrations of cholecystokinin in the intestine and the brain in young (4-8-month-old), and old (26-29-month-old) rats before and after intragastric administration of synthetic trypsin inhibitor (camostate) were examined. The total cholecystokinin content in the proximal intestine was significantly higher in old rats, whereas that in the brain was similar in young and old rats. Intragastric administration of camostate significantly increased the plasma cholecystokinin concentration and decreased the intestinal cholecystokinin content in both young and old rats. It also decreased the cholecystokinin content in the brain of old rats but not of young rats. These results suggest that the effect of aging on changes in the tissue content of cholecystokinin varies in different tissues.

Journal Article↗
PubMedDate not supplied

Evidence for the effect of aging on tissue contents of gastrin, cholecystokinin and somatostatin in the rat.

The changes associated with aging in the tissue concentrations of cholecystokinin, somatostatin and gastrin in young (7-month-old), middle-aged (13-month-old) and old (25-26-month-old) rats were investigated. The concentrations and total contents of somatostatin and gastrin significantly decreased in the antrum in 13- and 25-26-month-old rats compared with the young controls. The cholecystokinin concentration in the proximal intestine and its total content significantly increased in the old rats. Somatostatin concentrations in the intestine and cerebral cortex did not change with age. Cholecystokinin content in the cerebral cortex decreased in old rats because of the decrease of tissue wet weight. We conclude that the effects of aging on the changes of peptide concentrations in tissues vary depending on peptide specie and in different tissues studied.

Journal Article↗
PubMedDate not supplied

Effect of long-term exercise under restricted-feeding on intestinal content of cholecystokinin and on the pancreas in aging rats.

The effects of exercise (5000 m/day running from 100 to 600 days of age) on the cholecystokinin (CCK) content in the proximal intestine, and the enzyme and insulin contents of the pancreas were examined in food-restricted rats. Food restriction decreased the body weight and the wet weights of the pancreas and proximal intestine but not the wet weight of the stomach. Food restriction also decreased the chymotrypsin content of the pancreas but not its amylase content. The contents of enzymes in the pancreas were not affected by exercise. The insulin content of the pancreas was lower in lean rats produced by food restriction and/or exercise than in controls. Exercise increased the wet weight of the proximal intestine and the CCK content of the intestine. The increase in the CCK content may be due to compensational change in the efficiency of digestion of luminal nutrients induced by exercise.

Journal Article↗
PubMedDate not supplied

Gene expressions of cholecystokinin (CCK) and CCK receptors, and its satiety effect in young and old male rats.

Cholecystokinin (CCK), one of the first discovered gastrointestinal hormones, which stimulates pancreatic enzyme secretion and induces gallbladder contraction, is one of the most abundant neurotransmitter peptides in the brain and is implicated in satiety via CCK-A receptors. We compared the suppressive effect of central administration of CCK on food intake in young and old rats. The suppressive effect on food intake was enhanced in old rats. To examine the mechanism of this enhanced suppression, we measured the mRNA levels of CCK, CCK-A and CCK-B receptors in the cerebral cortex and the hypothalamus of young and old male rats. The mRNA level of CCK-A receptors in the hypothalamus decreased with age, whereas the mRNA levels of CCK-B receptors in the hypothalamus and cerebral cortex did not. The mRNA level of CCK in the cerebral cortex decreased significantly in old rats, although the decrease in the hypothalamus was not significant. Therefore, the enhanced sensitivity to CCK of old rats could not be explained by changes in gene expressions of CCK and CCK receptors. Moreover, the effects of aging on the gene expressions of CCK-A and CCK-B receptors were different.

Journal Article↗
PubMedDate not supplied

Sex difference in gene expressions of cholecystokinin (CCK) and CCK receptor in young and old rats.

The gene expressions of cholecystokinin (CCK) in the proximal small intestinal mucosa and CCK-A receptor in the pancreas were examined in male and female young (4-8 months old) and old (26-29 months old) rats. Their concentrations of CCK in the intestine were also compared. In males, but not in females, the mRNA levels of CCK in the intestine and those of the CCK-A receptor in the pancreas were significantly lower in old than young rats. In females, but not in males, the tissue content of CCK in the proximal intestinal mucosa was significantly higher in old rats. The pancreatic wet weight and trypsin activity in the proximal lumen were similar in young and old rats of both sexes. Therefore, changes in the regulations of gene expressions of CCK and the CCK receptor with age differ in male and female rats.

Journal Article↗
PubMedDate not supplied

Nonfunctioning adrenal rest tumor of the liver: radiologic appearance.

We describe the radiologic features of an adrenal rest tumor of the liver. The adrenal rest tumor appeared on ultrasound as a round, well defined, heterogeneous, solid mass in the posterior aspect of the liver, on angiography as a homogeneous hypervascular mass, and on dynamic CT as a mass containing components of both fat density and soft tissue density and showing early fill-in and early fill-out. Adrenal rest tumors should be included in the lists of hypervascular or fat-containing masses in the liver.

Adrenal Glands↗
PubMedDate not supplied

Campylobacter fetus subspecies fetus meningitis with chronic alcoholism and diabetes mellitus.

A case of Campylobacter fetus subspecies fetus meningitis is reported. The patient had underlying diseases, namely chronic alcoholism and diabetes mellitus. The infection did not respond to Piperacillin and Cefotaxime, but did respond to Ampicillin and Moxalactam. The patient was discharged on the 33rd hospital day showing no neurological deficit complications, and has remained free of recurrent disease for one month after the discontinuation of therapy.

Alcoholism↗