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Biomedical subjects

A Fukushima

Publications and source records attributed to A Fukushima.

At least 91 records · Page 5Linked to original sources
PubMed1994

Chromosomal assignment of HepG2 3'-directed partial cDNA sequences by Southern blot hybridization using monochromosomal hybrid cell panels.

Large-scale sequencing of a 3'-directed cDNA library from the human liver cell line HepG2 has generated several hundred species of cDNA gene signatures, about 85% of which identify novel genes. They are useful molecular landmarks for human genome mapping. We used 160 of these novel signatures as probes for Southern hybridization to human DNA. We then identified the copy number of the corresponding genes and assigned them to chromosomes, with reference to monochromosomal hybrid cell mapping panels. The distribution profile of the expressing genes among chromosomes suggested that the expressing gene density is not uniform.

Animals↗
PubMed1994

A human cDNA sequence homologue of bovine phosphatidylethanolamine-binding protein.

Sequencing of about 1000 3'-directed cDNA clones from the human HepG2 cell line revealed that about half of them represent transcripts of abundantly or moderately expressed genes, about 70% of which are novel. We identified one of these clones as encoding the human homologue of bovine phosphatidylethanolamine-binding protein.

Amino Acid Sequence↗
PubMed1994

Chromosomal assignments of novel genes expressed in HL60 granulocytes.

By collecting 3'-directed cDNA sequences called gene signatures (GSs) on a large scale, it is possible to make an expression profile of genes in a particular tissue, as well as discovering a number of novel genes. A total of 305 novel GSs collected from granulocytoid cells derived from HL60, a human promyelocytic leukemia cell line, by exposure to dimethyl sulfoxide were radiolabeled and used for Southern blot analyses to determine the copy number of the corresponding genes. Of these, 198 GSs identified as representing single-copy genes were then used as probes for hybridization analyses using a monochromosomal hybrid cell DNA panel. Sixty-nine of them were assigned to individual chromosomes. These results demonstrate that the chromosomal distribution of the GSs seems not to be proportional to the cytogenetic length of each chromosome.

Animals↗
PubMed1994

Antigenic cross-reactivity between human T lymphotropic virus type I (HTLV-I) and retinal antigens recognized by T cells.

To investigate the relationship between uveitis and HTLV-I infection, we examined the cross-reactivity between HTLV-I antigens and retinal antigens recognized by T cells in B10.BR mice immunized with human HTLV-I-infected MT-2 cells. We found that T cells obtained from MT-2 immune mouse spleen responded not only to HTLV-I antigens but also to retinal antigens of various species. However, they did not respond to HTLV-I-negative lymphoid cell lines. Furthermore, established T cell lines from MT-2 immune spleen cells also responded to both HTLV-I and retinal antigens. The phenotype of the immune cells that responded to both HTLV-I and retinal antigens was CD4+, CD8-, and CD3+. The proliferative response of T cell lines to HTLV-I as well as various retinal antigens, was clearly blocked by addition of anti-CD3, anti-CD4, or anti-I-Ak MoAbs, but not by anti-CD8 antibody. The established T cell lines from HTLV-I immune spleen cells were all found to be CD3+, TCR beta +, CD4+, CD8- cells by flow cytometric analysis. These results indicate that an epitope of HTLV-I antigens is cross-reactive to an epitope of retinal antigens extracted from either human retinoblastoma or normal murine, rat, and bovine retinae at a T cell recognition level.

Animals↗
PubMed1994

Criminal responsibility in amphetamine psychosis.

Historical changes in forensic psychiatric evaluation on criminal responsibility and proceedings in psychopathological findings of amphetamine psychosis are reviewed at first. The classification of amphetamine related mental disorders are proposed in 6 types. Among them, the clinical characteristics and psychopathological features of "Anxiety-situational reaction type" (Fukushima) are described. According to some reasonable grounds, offenders diagnosed as anxiety-situational reaction type should be evaluated as diminished responsibility in place of irresponsibility. Finally, two cases of murder committed under the influence of amphetamine, are reported in detail.

Adult↗
PubMed1994

[HTLV-I and retinal antigen recognized by T cells].

MT-2 immune but not naive murine (B10.BR) spleen cells responded not only to HTLV-I-infected cell lines but also to retinal antigens of various species by antigen-induced cell proliferation. The phenotype of the responding cells against HTLV-I-infected cell lines as well as retinal antigens was Thy-1.2+, CD4+ and CD8-. The antigen-induced cell proliferation against both HTLV-I-infected cell lines and retinal antigens was clearly inhibited by monoclonal antibodies to CD3, CD4, and MHC class II (I-AK). These data indicate that an epitope of HTLV-I-infected cell lines recognized by the CD4+ T cell is crossreactive to that of retinal antigens.

Animals↗
PubMed1993

An in vivo study of the replication origin in the influenza virus complementary RNA.

A new in vivo replication system for influenza virus was developed by using the clone 76 cell line, in which the viral RNA polymerase and nucleoprotein genes can be expressed in response to dexamethasone. The chimeric NS-chloramphenicol acetyltransferase (CAT) RNAs in the sense and antisense orientations positioned between the 5'- and 3'-terminal sequences of the influenza virus RNA segment 8 can be replicated [both genomic RNA (vRNA) and complementary RNA (cRNA) were transcribed] in the clone 76 cells treated with dexamethasone. These data indicate that three RNA polymerase proteins (PB1, PB2, and PA) and nucleoprotein are sufficient for replication of the influenza virus genome. Analysis of mutant cRNAs containing a base-substitution or a deletion in the 3'-conserved terminal 13 nucleotides revealed that important cis elements in the cRNA for vRNA synthesis reside at positions 2, 3, and 7 to 13 nucleotides from the 3'-end.

Animals↗
PubMed1993

Bilateral uveitis in a rabbit experimentally infected with human T-lymphotropic virus type I.

BACKGROUND: Among the various diseases associated with human T-lymphotropic virus type I (HTLV-I) infection, HTLV-I-associated uveitis has been proposed as a distinct clinical entity. EXPERIMENTAL DESIGN: Four rabbits experimentally infected with HTLV-I by blood transfusion from an HTLV-I-infected rabbit were observed for development of disease more than 3.5 years. Ophthalmologic, pathologic, hematologic, and virologic studies were performed on a rabbit that developed signs of uveitis. RESULTS: One of four rabbits developed bilateral uveitis 3.5 years after infection with HTLV-I. Ophthalmologic examination revealed opacities of the anterior chamber, lens, and vitreous body, as well as ciliary injection. Histologic examination demonstrated marked thickening of Descemet's membrane with degeneration of the corneal endothelial cells and mononuclear cell infiltration in the iris and choroid. The normal structure of the retina was almost completely replaced by gliosis. Gene amplification of cells from the left anterior chamber by polymerase chain reaction using pX primers disclosed the presence of HTLV-I sequences and an HTLV-I producing cell line (UV-1) was established from cells of the right anterior chamber. CONCLUSIONS: This is the first indication that rabbits may serve as an animal model of HTLV-I-related uveitis.

Animals↗
PubMed1992

Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression.

Large scale sequencing of cDNAs provides a complementary approach to structural analysis of the human genome by generating expressed sequence tags (ESTs). We have initiated the large-scale sequencing of a 3'-directed cDNA library from the human liver cell line HepG2, that is a non-biased representation of the mRNA population. 982 random cDNA clones were sequenced yielding more than 270 kilobases. A significant portion of the identified genes encoded secretable proteins and components for protein-synthesis. The abundance of cDNA species varied from 2.2% to less than 0.004%. Fifty two percent of the mRNA were abundant species consisting of 173 genes and the rest were non-abundant, consisting of about 6,600 genes.

Base Sequence↗
PubMed1992

[Analysis of DNA synthetic cells in endometrial carcinoma cases treated with progestogen].

It is well known that the proliferation of endometrial adenocarcinoma is inhibited by progestogens. We often use medroxyprogesterone acetate (MPA) as endocrine therapy for advanced endometrial carcinoma. In the present study, we administered 400-600mg of MPA/day on 14 days as a progestogen challenge test (PCT) to 37 cases of endometrial carcinoma. We analysed the variation in the percentage of S phase cells by flow cytometry and also conducted an immunohistochemistry analysis with anti-BrdU monoclonal antibody before and after the administration of MPA. The percentage of S phase cells in endometrial carcinoma tended to decrease with much greater variation after PCT than before. The percentage of BrdU positive cells tended to decrease after PCT. The cases with a good histological effect such as 1) basal vacuolization 2) clear staining cytoplasm 3) homogenus finding of cells and nucleus by PCT had a better prognosis than the cases with no histological effect due to PCT.

Adenocarcinoma↗
PubMed1989

Role of acetylcholine in pyridostigmine-induced myocardial injury: possible involvement of parasympathetic nervous system in the genesis of cardiomyopathy.

Although acetylcholine is known to be involved in the genesis of skeletal muscle disturbance, its effect on cardiac muscle has been scarcely studied. In the present paper, using pyridostigmine, a cholinesterase inhibitor, the possible role of acetylcholine in the genesis of cardiomyopathy was investigated. In a mortality study, it was shown that pyridostigmine (100 mg/kg) caused death of 9/10 rats within 8 h, and that the lethality of such a dose could be significantly diminished by the subsequent administration of a total dose of 4 mg/kg atropine. In all other experiments, rats were divided into three groups; the control, untreated group; the pyridostigmine + atropine group in which atropine (2 mg/kg) was administered 5 min after pyridostigmine (60 mg/kg) administration; and the pyridostigmine group in which pyridostigmine (60 mg/kg) was administered orally. Rats were killed 3 h after pyridostigmine administration, and hearts were isolated. Heart mitochondrial electron transport activity (NADH-cytochrome c reductase, succinate-cytochrome c reductase, and cytochrome c oxidase) were measured enzymatically, and mitochondrial respiratory rates and control indices were measured polarographically. Structural changes in cardiac muscles of each group were observed by electron microscopy of cardiac sections. Acetylcholine levels of left ventricle were measured by high performance liquid chromatography. Activities of NADH-cytochrome c reductase and succinate-cytochrome c reductase were not affected by pyridostigmine administration; however, cytochrome c oxidase activity was significantly reduced in the pyridostigmine group. Atropine markedly lessened this reduction in activity. A protective effect of atropine was also observed morphologically. A protective effect of atropine was also observed morphologically. In the pyridostigmine group and the pyridostigmine + atropine group, left ventricular acetylcholine levels were increased significantly compared with the control.(ABSTRACT TRUNCATED AT 250 WORDS)

Acetylcholine↗
PubMed1989

Acceleration of recovery of mitochondrial function after coronary reperfusion by various coronary dilating drugs in canine hearts.

This study was designed to evaluate whether or not increase in coronary blood flow after reperfusion accelerates the recovery of ischemia-induced mitochondrial damage. Using anesthetized dogs, the left anterior descending coronary artery was occluded for 30 min, followed by 20 min of reperfusion. Five minutes after reperfusion, either physiological saline (n = 9), 0.5 mg/kg of dilazep (n = 7), 0.2 mg/kg of diltiazem (n = 7), or 0.5 mg/kg of nicorandil (n = 8) were administered intravenously. Arterial blood pressure, heart rate, and coronary blood flow were measured throughout the experiment. Twenty minutes after reperfusion, heart mitochondria from normal and reperfused areas were prepared, and mitochondrial function was measured. Significant increase in coronary flow was observed during reperfusion in all drug-treated groups; however, no significant increase was observed in the control group 10 min after reperfusion. Significant hemodynamic changes were not observed in all groups. Mitochondrial function from reperfused areas was recovered significantly in all drug-treated groups, though in the control group mitochondrial dysfunction persisted. Coronary dilative mechanisms of drugs used here differ; however, a similar effect was demonstrated, i.e., administration of a coronary dilator accelerates the recovery of mitochondria after reperfusion. Therefore, it is concluded that coronary flow after reperfusion might be a primary factor in the recovery of ischemia-induced mitochondrial damage.

Animals↗
PubMed1989

[The effect of flecainide on 2 hour occlusion-induced myocardial damages in dog].

This study was designed to clarify whether or not flecainide have cardioprotective effect on ischemic myocardium. Nineteen dogs anesthetized and subjected to 2 h coronary occlusion, were divided into 2 groups. In the control group, physiological saline was infused, and in the flecainide group, 2 mg/kg of flecainide acetate and followed by 100 micrograms/kg/min continuous infusion. To assess the cardioprotective effect, mitochondrial function and lysosomal enzyme activities were measured. Two hours after occlusion, mitochondria were prepared from both ischemic and non-ischemic areas in each group, and their function (respiratory control index, and the rate of oxygen consumption in state III respiration) were measured polarographically with succinate as substrate. Fractionation of myocardial tissue from both non-ischemic and ischemic areas was performed according to the method of Weglicki et al, and the activities of lysosomal enzymes (N-acetyl-beta-glucosaminidase, and beta-glucuronidase) were measured. In the control group, mitochondrial dysfunction and leakage of lysosomal enzymes induced by 2 h occlusion were observed. On the contrary, administration of flecainide maintained significantly mitochondrial function, and prevented significantly leakage of lysosomal enzymes. These results indicated that flecainide had cardioprotective effect as well as antiarrhythmic effects in ischemic myocardium.

Animals↗
PubMed1988

Cardiovascular effects of leukotoxin (9, 10-epoxy-12-octadecenoate) and free fatty acids in dogs.

Leukotoxin (9,10-epoxy-12-octadecenoate) biosynthesised from linoleate by neutrophils is highly toxic to cellular function. Its cardiovascular effects were studied in dogs together with the effects of various fatty acids. Aortic flow, left ventricular peak dP.dt-1, and aortic pressure were measured in 60 anaesthetised dogs, which were divided into 10 groups of six animals each--namely, control group (10 ml of physiological saline), three leukotoxin groups (5, 10, and 50 mg.kg-1), two linoleic acid groups (10 and 50 mg.kg-1), two oleic acid groups (10 and 50 mg.kg-1), and two stearic acid groups (10 and 50 mg.kg-1). Leukotoxin injected intravenously depressed cardiac function in a dose dependent manner. Administration of leukotoxin 5 mg.kg-1 showed no significant cardiotoxic effect. However, 10 mg.kg-1 of leukotoxin significantly decreased aortic flow from 0.74(0.04) to 0.40(0.07) litre.min-1 (mean(SEM], left ventricular peak dP.dt-1 from 2040(205) to 1140(217) mmHg.s-1, and aortic pressure from 106(7.1)/67(6.3) to 75(9.2)/48(6.5) mmHg 5 min after injection. Dogs given leukotoxin 50 mg.kg-1 showed more pronounced cardiodepressive effects; aortic flow was decreased to 0.19(0.06), left ventricular dP.dt-1 to 560(134), and aortic pressure to 72(15.1)/41(10.6) 5 min after injection. All dogs in this group were dead within 45 min. Administration of 10 mg.kg-1 of linoleic acid, oleic acid, or stearic acid caused no significant haemodynamic changes. Administration of linoleic acid 50 mg.kg-1 had cardiotoxic effects, but the effect was less than that of leukotoxin. Since leukotoxin appears to be a potent cardiodepressive agent it may be an important factor in the development of heart failure observed in patients with severe burns.

Animals↗