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Biomedical subjects

A Fujii

Publications and source records attributed to A Fujii.

At least 109 records · Page 6Linked to original sources
PubMed1995

[Clinicopathological study of incidental renal cell carcinoma].

BACKGROUND: The objective of this study is to evaluate the clinicopathological features of incidental renal cell carcinoma, compared with non-incidental carcinoma. METHODS: Between July 1st, 1984 and June 30, 1994, 87 renal cell carcinoma patients were treated at our hospital; 56 had non-incidental renal cell carcinoma and 31 had incidental carcinoma. The clinicopathological features were examined. RESULTS: The incidence of incidental cancer ranges from 0 to 66%, and the incidence has increased in recent years. The median value of maximal tumor size was 4.0cm (1.5 approximately 8.0cm) for incidental cancer, and 8.0cm (3.0 approximately 16cm) for incidental cancer, and 8.0cm (3.0 approximately 16cm) for non-incidental cancer, i.e., the incidental cancer was significantly smaller than the non-incidental one (p < 0.001). The pathological stage of the resected non-incidental renal cell carcinoma (n = 47) was pT1, pT2, pT3 and pT4 in 0, 23, 21 and 3 patients, respectively. For the resected incidental renal cell carcinoma (n = 31) 3, 26, 2 and 0 patients showed pathological stages pT1, pT2, pT3 and pT4, respectively; the pathological stage of incidental renal cell carcinoma was significantly lower than that of non-incidental carcinoma (p < 0.001). Eighteen and 29 resected non-incidental renal cell carcinoma were grades 1 and 2, respectively, whereas 17 and 14 resected incidental renal cell carcinomas were in grades 1 and 2, respectively. Vascular invasion by tumor cells was shown in 31 (66.0%) and 8 (25.8%) patients with non-incidental and incidental renal cell carcinomas, respectively; the incidence of vascular invasion in incidental cancer being significantly lower than in non-incidental cancer (p < 0.001). The performance status and general condition in patients with incidental renal cell carcinoma were superior to those in patients with the non-incidental cancer. The 1, 3 and 5-year survival rate of all 87 renal cell carcinoma patients was 81, 62 and 57%, respectively. These rates for patients with non-incidental renal cell carcinoma were 72, 48 and 41%, respectively, and those for incidental cancer patients were 100%. The survival of patients with incidental renal cell carcinoma was significantly better than that of non-incidental carcinoma patients (p < 0.005). CONCLUSION: Our results suggest that the detection of incidental renal cell carcinoma will increase, and that the prognosis for renal cell carcinoma will improve. However, even in incidental renal cell carcinoma, careful long-term follow up may be necessary, since some tumors are comparatively large and exhibit vascular invasion.

Adult↗
PubMed1995

[Pulmonary involvement in patients with myeloperoxidase specific-antineutrophil cytoplasmic antibody].

In order to examine the clinical features of pulmonary involvement in patients with myeloperoxidase specific-antineutrophil cytoplasmic antibody (MPO-ANCA), 46 MPO-ANCA positive patients with collagen-vascular disease and glomerulonephritis were investigated. Twenty eight patients (60.8%) had pulmonary involvement without tumor and infection, 20 (43.5%) of MPO-ANCA positive patients had interstitial pneumonitis, 11 (23.9%) had pulmonary hemorrhage, 3 had asthma (6.5%) and 7 had both interstitial pneumonitis and pulmonary hemorrhage. Patients with pulmonary involvement were older (mean age 61.1) and they had higher inflammatory acute phase reactants and higher mortality (42.9%) than patients without pulmonary involvement. Pulmonary hemorrhage revealed on both lung fields in 10 patients, 7 patients required artificial respirator and 6 died. Titer of MPO-ANCA increased paralleled with progression of pulmonary hemorrhage. Interstitial fibrosis revealed predominantly lower and lateral side of the lung. It is suggested that MPO-ANCA may be closely related to the pathogenesis of pulmonary hemorrhage and interstitial pneumonia. Careful management is needed in patients with pulmonary involvement, especially pulmonary hemorrhage in patients with MPO-ANCA.

Adult↗
PubMed1995

Tenidap, an anti-inflammatory agent, discharges intracellular Ca++ store and inhibits Ca++ influx in cultured human gingival fibroblasts.

The effect of tenidap, (+/-)-5-chloro-2,3-dihydro-3-(hydroxy-2- thienylmethylene)-2-oxo-1H-indole-1-carboxamide, a new anti-inflammatory agent, was investigated on intracellular free Ca++ concentration ([Ca++]i) responses evoked by bradykinin and thapsigargin in gingival fibroblasts. Tenidap itself stimulated [Ca++]i response in a dose-dependent manner in the absence of extracellular Ca++. The pretreatment with tenidap inhibited [Ca++]i responses evoked by 5 nM bradykinin and 1 microM thapsigargin in a dose-dependent manner. This indicates that tenidap discharges intracellular Ca++ store, resulting in a depletion of intracellular Ca++ store. Tenidap partially depressed Ca++ influx across plasma membrane by 1 to 2 min pretreatment and almost completely by more than 5 min pretreatment. Thus, tenidap appears to be a valuable agent that functions on inhibition of Ca++ influx in nonexcitable cells, which is rare at the present time.

Aged↗
PubMed1995

[A clinicopathological study of angiogenesis in breast cancer].

To evaluate the clinicopathological significance and the objective methods for angiogenesis, we immunohistochemically stained all of the regions around the cancer nests with Factor VIII-related antigen staining. We also identified microvessels in the many areas all along the border between cancer nests and the stroma by 200 x power field. We then examined the relationship between the average microvessel count (AC/mm2) and clinicopathological factors and between a 10-year cumulative survival rate in 109 cases of primary breast cancer from 1971 to 1979. There were an average of 35 fields counted among all the cases, and AC was 39.1 +/- 16.1. AC without blood vessel invasion was 36.4 +/- 15.2, while AC with blood vessel invasion was 44.7 +/- 16.6 (p < 0.02). AC without node metastasis or with n1 alpha was 37.6 +/- 15.4, while AC with n1 beta or n2 or n3 was 45.2 +/- 17.4 (p < 0.05). There was no relationship between AC and clinical tumor size, histological classification, lymphatic invasion, nuclear grade, and histological extension. A 10-year cumulative survival rate in AC (> or = 39) was 62.4%, while that in AC (< 39) was 82.7% (p < 0.03). Thus AC was considered a useful prognostic factor.

Adult↗
PubMed1995

[Management of total laboratory system and its efficacy].

The Osaka City University Hospital was reopened in May 1993. In the Central Clinical Laboratory our Total Laboratory Automation System, a comprehensive online analyzing system, covers the entire process from collecting blood to completing tests. Our study showed that an average of 2,800 outpatients were examined per day in 1994, compared with 1,900 per day in 1992, an increase of 47.4%. The number of tests performed by the central laboratory in 1994 was 5,610,000 as compared to 3,720,000 in 1992, an increase of 50.8%. After the installation of the transport line and computerization the following can be noted: 1) We were able to extend reception hours by two hours. 2) We are now able to assign lab medical technologists to physiology analysis, emergency analysis, and initial outpatient testing. We conclude that these effects have been very large.

Clinical Laboratory Information Systems↗
PubMed1994

Glial extracellular matrix modulates gamma-glutamyl transpeptidase activity in cultured bovine brain capillary and bovine aortic endothelial cells.

Glial extracellular matrix (ECM) elevated gamma-glutamyl transpeptidase (gamma-GTP) activity in cultured bovine brain capillary and aortic endothelial cells (BBCEC, BAEC). In particular, the ECM of glial cells cultured with the conditioned medium of BAEC (BAEC CM) dramatically elevated gamma-GTP activity in BBCEC and BAEC. The ECM of glial cells cultured with BBCEC CM also had a marked effect. The ECM of 3T3 cells cultured with BAEC CM, and the ECM of glial cells cultured with 3T3 CM had no effect. Glial CM had no effect on gamma-GTP activity in BBCEC and BAEC. These findings indicate that gamma-GTP activity in endothelial cells (EC) is modulated by glial ECM, and that the factor of ECM that affects gamma-GTP activity in EC arises from the interaction between glial cells and EC.

Animals↗
PubMed1994

Effect of calcium-channel blockers on cell proliferation, DNA synthesis and collagen synthesis of cultured gingival fibroblasts derived from human nifedipine responders and non-responders.

Human gingival fibroblasts from six patients who developed gingival hyperplasia as a result of nifedipine medication and five who did not were studied for the effects of calcium-channel blockers (nifedipine, diltiazem, verapamil and nicardipine) on cell proliferation, DNA synthesis and collagen synthesis. Phenytoin was used as a positive control. The fibroblasts from reactive patients gave trends toward better cell proliferation rates, DNA synthesis ([3H]-thymidine incorporation), and collagen synthesis ([3H]-proline incorporation) than those from non-reactive patients in the presence of 1 microM of calcium-channel blockers or phenytoin.

Adult↗
PubMed1994

Malignant gonadal stromal tumor.

A case of malignant gonadal stromal tumor is reported, and the 24 cases reported in the English and Japanese literature are reviewed. A thirty-four-year-old male visited our hospital with a painless tumor in the left scrotum. Left high orchiectomy was performed under a diagnosis of testicular tumor, and histologic examination of the resected specimen demonstrated that it was a malignant gonadal stromal tumor. There has been no evidence of disease for forty-one months following surgery. Of the 15 cases reported in the English literature, retroperitoneal lymph node dissection was performed in 3 cases, and metastasis was present in the resected lymph nodes in 2 of the cases. These 3 patients survived with no evidence of disease for follow-up periods ranging between six months and five years. However, lymph node metastasis was observed in 5 of 6 patients who underwent orchiectomy alone or in combination with radiation to the retroperitoneal lymph nodes. The 3 patients described in the Japanese literature who had received high orchiectomy and retroperitoneal lymph node dissection survived with no evidence of disease for follow-up periods ranging from eighteen months to forty-one months following surgery. These findings indicate that retroperitoneal lymph node dissection is useful as a treatment for malignant gonadal stromal tumor. However, the prognosis for patients with advanced disease is very poor, because neither chemotherapy nor radiotherapy is effective. Corroborative study of many patients is necessary to understand the pathophysiology of malignant gonadal stromal tumor and to develop useful treatments.

Adult↗
PubMed1994

Cefadroxil concentrations in human serum, gingiva, and mandibular bone following a single oral administration.

Cefadroxil concentrations in human serum, gingiva, and mandibular bone were measured by a paper disk method following a single 500-mg oral dose. The mean peak concentrations in serum, gingiva, and mandibular bone occurred at the identical time, 3 hours, and were 12.92 micrograms/mL, 6.50 micrograms/g, and 2.67 micrograms/g, respectively. Mean cefadroxil concentration ratios of gingiva/serum and mandibular bone/serum at the peak time were 0.54 and 0.21, respectively. Mean concentrations in gingiva and mandibular bone at the peak time exceeded the minimum inhibitory concentrations for 90% of clinically isolated strains of a alpha-hemolytic streptococci.

Absorption↗
PubMed1994

Amoxicillin concentration in pus from abscess caused by odontogenic infection.

1. Amoxicillin concentration in pus from odontogenic infection was assayed and the concentrations were compared with MIC (minimum inhibitory concentration) of alpha-hemolytic streptococci isolated from odontogenic infection. 2. Measurable amoxicillin concentrations in serum and pus were found in all instances (n = 16). 3. The mean peak concentrations in serum and pus were found at identical times, 1.5 hr after administration, which were 5.92 and 0.90 micrograms/ml, respectively. 4. The mean concentration ratio of pus/serum at the peak time was 0.15. 5. All amoxicillin concentrations in pus at the peak time exceeded the MIC for 90% of alpha-hemolytic streptococci (0.25 micrograms/ml).

Administration, Oral↗
PubMed1994

Cephalexin concentrations in radicular granuloma following a single oral administration of 250- or 500-mg cephalexin.

1. Cephalexin concentrations in radicular granuloma and serum following a single oral administration of 250- or 500-mg cephalexin were measured by a paper disk method. 2. The highest concentration of cephalexin in radicular granuloma following administration of 250-mg cephalexin to nonfasting patients was observed at 2 hr, and was 1.62 micrograms/g. The mean cephalexin concentration ratio of radicular granuloma/serum at 2 hr was 0.35. 3. The highest concentrations of cephalexin in radicular granuloma following administration of 500-mg cephalexin to nonfasting and fasting patients occurred at 2 and 1.5 hr, and was 3.35 and 3.42 micrograms/g, respectively. Mean cephalexin concentration ratios of radicular granuloma/serum at 2 and 1.5 hr were 0.32 and 0.30, respectively. 4. All mean cephalexin concentrations in radicular granuloma following administration of 500-mg cephalexin to both fasting and nonfasting patients exceeded MIC for 90% (2 micrograms/ml) of clinically isolated strains of alpha-hemolytic streptococci. However, those concentrations obtained by 250-mg cephalexin did not exceed it.

Adult↗
PubMed1994

Amino acids and peptides. XXXVII. Synthesis of stereoisomeric nonapeptides corresponding to sequence 41-49 of eglin c and examination of their inhibitory activity against human leukocyte cathepsin G and alpha-chymotrypsin.

A nonapeptide, H-Ser-Pro-Val-Thr-Leu-Asp-Leu-Arg-Tyr-OH, corresponding to sequence 41-49 of eglin c inhibited leukocyte cathepsin G and alpha-chymotrypsin with Ki values of 2.2 x 10(-5) and 7.2 x 10(-6) M, respectively, although eglin c itself inhibited leukocyte elastase, cathepsin G and alpha-chymotrypsin with Ki values of 6.0 x 10(-9), 5.5 x 10(-9) and 2.5 x 10(-9) M, respectively. The inhibitory activity of the nonapeptide decreased following incubation with cathepsin G due to the cleavage of the Leu45-Asp46 peptide bond. Therefore, Leu45 and/or Asp46 were replaced with D-amino acids and the inhibitory activities of the resultant nonapeptides were examined. Their inhibitory activities against cathepsin G and alpha-chymotrypsin were much weaker than those of the all-L-type nonapeptide, suggesting that the amino acids at the active site, Leu45 and Asp46 are required to be in the L-configuration for potent activity.

Amino Acid Sequence↗
PubMed1994

[Retrospective analysis of relationship between progression and regression of dysplasia and existence of HPV DNA by in situ hybridization].

We investigated the relationship between the progression and regression of cervical dysplasia and the existence of HPV DNA. The materials we analyzed were 80 biopsied specimens obtained from 22 cases of dysplasia which were followed-up for a long time at our out-patient clinic, and 47 specimens of squamous cell carcinoma. Detection of HPV DNA was done by in situ hybridization with biotinylated HPV DNA probes types 6/11, 16/18 and 31/33/35. The analysis of several specimens obtained at long term follow-up showed that the dysplasia-progression group had a higher incidence and more frequent detection of HPV DNA than the regression group. In the progression group, HPV 16/18 were detected more frequently than HPV 31/33/35 at the dysplasia lesion, but HPV 6/11 was detected in neither the dysplasia nor the squamous cell carcinoma. Fourteen of the 47 cases of squamous cell carcinoma (29.8%) were positive for HPV DNA, and 13 of them (92.9%) were detected in the lesions of dysplasia adjacent to carcinoma in situ or invasive cancer. Comparison of the detection rate for HPV DNA was done in the 3 dysplastic areas, i.e. the dysplastic areas in the dysplasia progression group (A), dysplasia regression group (B) and in the area adjacent to squamous cell carcinoma (C). The detection rates for HPV DNA were 43.5% in A, 27.7% in C and 15.0% in B. From these results we drew the following conclusions: 1) The continuous existence of HPV DNA, especially type 16/18 in the dysplasia lesions, may progress from lesions to carcinoma in situ or invasive cancer.(ABSTRACT TRUNCATED AT 250 WORDS)

Carcinoma, Squamous Cell↗
PubMed1994

[Clinical study of total prostatectomy for prostatic cancer].

Total retropubic prostatectomy was performed on 31 patients with prostatic cancer (median age; 70 years). The accuracy of staging was 55% (17 patients). In 11 patients (35%) the staging had been underestimated, and in 3 patients (10%) overestimated. The pathological stage and grade were stage B in 14 patients, stage C in 4 patients, stage D1 in 13 patients (pN1; 8 patients, pN2; 5 patients), well differentiated adenocarcinoma (WDA) in 8 patients moderately differentiated adenocarcinoma (MDA) in 7 patients, and poorly differentiated adenocarcinoma (PDA) in 16 patients. The WDA were all stage B, and 85% of the PDA were stage D1. The positive surgical margin rate was 21% in stage B, 50% in stage C, 85% in stage D1, 13% in WDA, 43% in MDA, and 75% in PDA. Five of the patients died, and the causes of death were prostatic cancer in 2 patients (pN2/PDA), and others in 3 patients (1 patient; stage B/PDA, 2 patients; pN2/PDA). The 5-year survival rate (Kaplan-Meier) was 84% on the whole, 83% for stage B, 100% for stage C, 83% for stage D1, 100% for WDA, 100% for MDA, 71% for PDA, 86% for pN0, 100% for pN1, and 38% for pN2.

Adenocarcinoma↗
PubMed1994

A case of papillary renal cell carcinoma suggestive of Bellini duct origin.

A case of Bellini duct carcinoma is reported. A left renal tumor was detected by abdominal computerized tomography in a 76-year-old male, although he had no symptoms, such as hematuria, weight loss or flank pain. Radical nephrectomy was performed under the diagnosis of renal cell carcinoma in the left kidney. Macroscopic examination of the resected kidney revealed a tumor 2.0 cm in diameter, with a yellow-brown cut surface, located in the renal medulla. Histological examinations showed malignant tumor cells with eosinophilic cytoplasm with a papillary growth pattern. Immunohistostaining examinations using Lectin and two kinds of monoclonal antibodies demonstrated no significant staining with soybean agglutinin, peanut agglutinin, Dolichos biflorus agglutinin, Lotus tetragonolobus agglutinin or cytokeratin, and negative staining with Tamm-Horsfall protein. Although the results of immunohistostaining did not provide support, both macroscopic and microscopic findings strongly suggested that this tumor originated from Bellini duct epithelium (Bellini duct carcinoma). The patient is alive with no evidence of disease 1 year after surgery. Bellini duct carcinoma is a rare malignant condition and the prognosis is usually poor. Differential diagnosis from other renal or pelvic tumors is difficult and long-term careful follow-up is necessary.

Aged↗
PubMed1993

Josamycin concentrations in radicular cysts following a single oral administration.

1. Josamycin concentrations in cyst wall (wall) and cyst fluid (fluid) of radicular cyst and serum following a single oral administration of josamycin (600 mg) were assayed by a paper disk method. 2. The mean peak josamycin concentrations in wall, fluid and serum occurred at 1.5, 2 and 1.5 hr, respectively, and were 1.33 micrograms/g, 0.53 and 0.74 micrograms/ml, respectively. 3. The mean concentration ratios of wall/serum, fluid/serum and fluid/wall at the peak times (1.5, 2 and 2 hr) were 1.80, 0.64 and 0.42, respectively. 4. Josamycin concentrations in wall and fluid at the peak time exceeded MIC for 80% for clinically isolated strains of alpha-hemolytic Streptococci.

Administration, Oral↗