Search PubMed⌕ Search

Biomedical subjects

A Fujii

Publications and source records attributed to A Fujii.

At least 271 records · Page 15Linked to original sources
PubMed1981

[Antitumor activities of bamboo leaf extracts (BLE) and its lignin (BLL)].

Antitumor activity of bamboo leaf against various transplantable mouse tumor strains, such as Sarcoma 180 and Ehrlich ascites carcinoma has previously been demonstrated by Sakai et al.7) and Yammamoto et al.9). The present investigation was undertaken to determine the antitumor activities of BLE and BLL against the spontaneous tumor induced by benzopyrene (BP) and 4-nitroquinoline-1-oxide (4NQO) in mice and rats. The possible mechanism of antitumor action was also discussed as related to Kada's Rec-assay and Ames test in vitro. The in vivo antitumor test was performed using 16 groups of mice given the following solutions ad libitum for 120 days; Groups 1-4, 5-8, 9-12 and 13-16 were given water, 1% and 10% BLe, 0.1% BLL, and each respective group was treated with no injection (control), oil/s.c., BP 1 mg/s.c., and 4NQO 0.5 mg/s.c. Rat respective group was treated 30 days after in initiation of experiment with no injection (control), oil/s.c., BP 2.4 mg/s.c. Antitumor activities of the BLE and BLL were determined by the tumor incidence index and average weight of tumor. In vitro Rec-assay, cold incubation method, and Ames test were performed in the usual manner. Antitumor activity against BP induced tumor was the highest with 1.0% BLE (0.71 mg/ml), but no significant difference was found between the groups of the 10% BLE, 0.1% BL and control. A weak trend toward DNA damage was seen in the case of BLE, in the Rec-assay. His+ revertants with S-9 mix on Salmonella typhimurium TA98 were found in the case of BLL. It was concluded that antitumor activity against BP- and 4NQO-induced tumors was the highest with 1% BLE (0.71 mg/ml), and a direct action of BLE on tumor cells was indicated.

4-Nitroquinoline-1-oxide↗
PubMed1981

A fundamental study of regional chemotherapy given by intraarterial infusion with concomitant hemodialysis and hemoperfusion.

In the intraarterial infusion of anticancer drugs, the majority of systemic side effects are caused by the systemic circulation of the infused drug. If the extraregional infusate could be removed by hemodialysis (HD) or direct hemoperfusion (DHP), side effects in the intraarterial infusion therapies could be mitigated and regional administration of anticancer drugs in large doses would than be possible. To substantiate this postulate, the authors carried out in vivo experiments using mongrel dogs, and obtained the results evidencing removal of extra regional anticancer drugs by HD and DHP. The maximum mitomycin C clearance of HD was 35.7 ml per min, whereas that of DHP was 119.7 ml per min. The results show the usefulness of concomitant use of HD or DHP in the intraarterial infusion of anticancer drugs via internal iliac artery in the treatment of cancer of the urinary bladder.

Animals↗
PubMed1980

Chemistry of bleomycin. XXVI. Biosynthetic study using 13C-enriched precursors.

3-Morpholinopropylamino-bleomycin (MOP-BLM) was produced by the culture of Streptomyces verticillus ATCC 15003 grown in the presence of a 13C-enriched compound and 3-morpholinopropylamine in the medium, and the resulting MOP-BLM was analyzed by 13C-NMR spectrometry. In the spectrum of MOP-BLM produced by addition of 13C-methyl-L-methionine, the intensities of two signals assigned to the methyl carbon of the pyrimidine chromophore and of the 2-position of the 4-amino-3-hydroxy-2-methylpentanoic acid moiety were remarkably enhanced. When DL-alanine-3-13C was added, the signals emanated from the 5-methyl and 2-methine carbons of the 4-amino-3-hydroxy-2-methylpentanoic acid moiety were markedly enhanced. These results definitely indicate that the methyl group of the pyrimidine moiety originates from methionine-methyl group and the carbon skeleton of the pentanoic acid moiety is formed from alanine, acetate and methionine-methyl group. This study also revealed that the former assignment of the two signals from the two methyl carbons of the 4-amino-3-hydroxy-2-methylpentanoic acid moiety must be reversed.

Bleomycin↗
PubMed1979

Deleterious effect of short-term exposure to Coca-Cola on rats.

Semiacute toxicity of cola fluid, Coca-Cola, conducting for approximately a month in rats, was studied. A trend of cariogenicity of Coca-Cola was strongly indicated. When the fluid was given ad libitum, decarbonized Coca-Cola and carbohydrate solution, consisted 8% of glucose and 3.5% of sugar and then adjusted pH to 2.4 with oxalic acid, were consumed 2 to 3 times greater than the control (water). A hyperuresis was observed as the result of great consumption of liquid, but no liver nor kidney degeneration was observed by histopathological examination. The diet consumption of the groups of Coca-Cola and carbohydrate solution was approximately a half of the control, water. However, when a complete diet was given, no physiological difference in time was observed, except in diarrhea and depression of hair gloss in Coca-Cola group.

Animals↗