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Biomedical subjects

A Fuchs

Publications and source records attributed to A Fuchs.

At least 19 recordsLinked to original sources

A supraomohyoidal plexus block designed to avoid complications.

Interscalene blocks of the brachial plexus are used for surgery of the shoulder and are frequently associated with complications such as temporary phrenic block, Horner syndrome or hematoma. To minimize the risk of these complications, we developed an approach that avoids medially directed needle advancement and favors spread to lateral regions only: the supraomohyoidal block. We tested this procedure in 11 cadavers fixed by Thiel's method. The insertion site is at the lateral margin of the sternocleidomastoid muscle at the level of the cricoid cartilage. The needle is inserted in the axis of the plexus with an angle of approximately 35 degrees to the skin, and advanced in lateral and caudal direction. Distribution of solution was determined in ten cadavers after bilateral injection of colored solution (20 and 30 ml) and followed by dissection. In an eleventh cadaver, computerized tomography and 3D reconstruction after radio contrast injection was performed. In additional five cadavers we performed Winnie's technique with bilateral injection (20 and 30 ml). Concerning the supraomohyoidal block the injection mass reached the infraclavicular region surrounded all trunks of the brachial plexus in the supraclavicular region and the suprascapular nerve in all cases. The solution did not spread medially beyond the lateral margin of the anterior scalene muscle into the scalenovertebral triangle. Therefore, phrenic nerve, stellate ganglion, laryngeal nerve nor the vertebral artery were exposed to the injected solution. Distribution was comparable with the use of 20 and 30 ml of solution. Injections on five cadavers performing the interscalene block of Winnie resulted in an extended spread medially to the anterior scalene muscle. We conclude that our method may be a preferred approach due to its safety, because no structures out of interest were reached. Solution of 20 ml is suggested to be enough for a successful block.

Brachial Plexus↗

Atypical symptoms in patients with herpesvirus DNA detected by PCR in cerebrospinal fluid.

BACKGROUND: Polymerase chain reaction (PCR) detection of herpesvirus DNA in cerebrospinal fluid (CSF) is an important tool in the diagnosis of central nervous system (CNS) syndromes. The corresponding viral infections present with diverse clinical signs, which are often classical although no sign can be considered as specific. This retrospective study aims to describe atypical symptoms in patients with herpesvirus DNA detected in CSF by PCR. A total of 3452 cerebrospinal fluid samples from patients with suspected herpesvirus infection of the CNS were investigated between 1998 and 2003 in our clinical virology laboratory. "In-house" PCRs for each herpesvirus [herpes simplex virus (HSV), varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein Barr virus (EBV), or human herpes virus 6 (HHV6)] were used until 2001 and a commercially available "Herpes Consensus PCR" was used thereafter. One of the five herpesviruses investigated in this study was found in 71 (2.1%) of CSF samples (37 HSV, 14 VZV, 1 CMV, 9 EBV and 10 HHV6). These samples were obtained from 62 patients whose clinical findings were generally consistent with the PCR data. However, some little known features of herpesvirus-related symptoms, such as partial seizure associated with HSV infection, and unusual VZV or HHV6-related myelitis were also observed.

Adolescent↗

A topologically faithful, tissue-guided, spatially varying meshing strategy for the computation of patient-specific head models for endoscopic pituitary surgery simulation.

This paper presents a method for tessellating tissue boundaries and their interiors, given as input a tissue map consisting of relevant classes of the head, in order to produce anatomical models for finite element-based simulation of endoscopic pituitary surgery. Our surface meshing method is based on the simplex model, which is initialized by duality from the topologically accurate results of the Marching Cubes algorithm, and which features explicit control over mesh scale, while using tissue information to adhere to relevant boundaries. Our mesh scale strategy is spatially varying, based on the distance to a central point or linearized surgical path. The tetrahedralization stage also features a spatially varying mesh scale, consistent with that of the surface mesh.

Algorithms↗

[A comparison of different methods of performance assessment for the course in general medicine].

A novel Federal Regulation for Basic Medical Education was issued in Germany, which requires an end of course assessment. General Practice (GP) in Duesseldorf has introduced a written examination. An accompanying study was carried out to understand whether different methods of performance evaluation would show equivalent results intraindividually. Out of the GP class 21 students were randomised to undergo an oral examination on top of the regular written examination. Furthermore their teaching practitioners have appraised these students performance in small group teaching. Finally, the students have been rated following a practical term. At the end the students have been interviewed in a focus group to investigate their preferred method of assessment. The survey of the average grades showed comparable results for the written test, the oral examination and small group teaching while the appraisal at the end of the practical term happened to be significantly higher in grade. The comparison of the individual results, however, showed a statistical spreading in all directions. For example, a poor performance in the written test can come along with excellent results in the oral examination and vice versa. The intra-class correlation across all classes has been as low as 0.1. These results support the conclusion that apparently each method can appropriately evaluate only a very specific part of the complex contents of the GP education and of students' performance. In order to properly examine the performance of GP students a combination of different evaluation methods needs to be applied. As an alternative, different skills can be examined in different practical and theoretical test situations in an OSCE.

Curriculum↗

Assessing recovery in middle cerebral artery stroke using functional MRI.

PRIMARY OBJECTIVE: To understand the temporal evolution of brain reorganization during recovery from stroke. RESEARCH DESIGN: A patient who suffered left middle cerebral artery stroke 9 months earlier was studied on three occasions, approximately 1 month apart. METHODS AND PROCEDURES: Brain activation was studied using functional Magnetic Resonance Imaging (fMRI). During each session, the patient performed a finger-to-thumb opposition task, which involved one bimanual and two unimanual conditions. Each condition consisted of overt movement of fingers and imagery of the same task. RESULTS: With recovery, greater recruitment was observed of the affected primary motor cortex (M1) and a decrease in activation of the unaffected M1 and supplementary motor area. In addition, the widespread activation of brain areas seen during the initial session changed to a more focused pattern of activation as the patient recovered. Imagery tasks resulted in similar brain activity as overt execution pointing to imagery as a potential tool for rehabilitation.

Aged↗

[Orthotics and cerebral palsy. Established treatments and trends in orthopaedic devices for patients with cerebral palsy].

The surgical and pharmacological treatment of cerebral palsy patients is, in many cases, complemented by orthopaedic appliances. New knowledge and materials have expanded the possibilities for orthotic treatment in the last years, but have also led to confusion on the correct technology to use in different cases. This paper presents an overview of the current orthotic methods in cerebral palsy patients. Initially, we present the different ideas and show the limitations of the treatment. Next, we consider the problem of spastic hip dislocation and the possibilities of positioning the patients. Beds and splints cut from foam allow safe positioning of severely disabled patients without the risk of pressure sores. This appliance may prevent spastic hip dislocation in the long-term. Results of a patient questionnaire are presented. The third section deals with experiences with full contact braces and differences in their construction compared to conventional corsets. Our own results from a patient questionnaire and clinical cases will be presented. The use of a full contact brace may have a positive influence on the development of the spastic scoliosis.

Ankle↗

[Imaging of the coronary arteries with multi-channel CT: a pictorial assay].

Advances in cardiac imaging with multidetector row CT allow new applications in the diagnosis of cardiac pathology. The purpose of this article is to illustrate the normal anatomy and main anatomical variants of the coronary arteries that can be detected with multi-channel CT. The images presented here were obtained using 4-channel and 16-channel multi-detector row CT units. Post-processing of CT imaging data was performed using: VRT, MPVR, and 2D curved MPR. Because of continuing technical advances, the use of CT imaging in the evaluation of cardiac disorders is likely to increase.

Coronary Angiography↗

Production of interleukin-18 and interleukin-12 in patients suffering from chronic hepatitis C virus infection before antiviral therapy.

Interleukin-12 and -18 (IL-12 and IL-18) are known to enhance the cytotoxic T-lymphocyte response synergistically, but their precise involvement in hepatitis C virus (HCV) infection is not well known, especially for IL-18. Enzyme-linked immunosorbent assay (ELISA) was used to study the production of these cytokines in plasma and peripheral blood mononuclear cells (PBMCs) stimulated by lipopolysaccharide (LPS) of patients infected chronically with HCV before initiation of antiviral therapy. Fifty-six patients and 40 healthy controls were evaluated. Patients infected with genotype 1 or with genotype other than genotype 1 HCV had significantly a high production of plasma IL-12 compared with controls (P < 0.05). However, patients infected with genotype 1 HCV had lower levels of PBMC IL-18 than were founded in the controls (P < 0.05); plasma IL-18 also tended to be lower in this group of patients than in the controls, although nonsignificantly. Plasma IL-18 was related to hepatic histological activity (P < 0.05). The data suggest a relationship between these two cytokines and some features of HCV infection, so that their respective production in relation to the outcome of the infection deserves further study.

Adolescent↗

Prevalence and persistence of TT virus DNA in HIV1-infected individuals.

BACKGROUND: TT virus (TTV) is a recently discovered virus with a high DNA prevalence in different populations. Its role in pathogenesis is uncertain, particularly in immunocompromised patients. PATIENTS AND METHODS: Prevalence of TTV-DNA was evaluated in a cohort of HIV-infected patients and in blood donors by nested PCR, using two different primer sets: T primers, derived from the open reading frame ORF1 region N22; B primers, derived from the untranslated region (UTR). Samples positive using T primers were also tested for TTV-DNA in peripheral blood mononuclear cells (PBMC) and followed up every 4 months. RESULTS: The overall prevalence of TTV-DNA in HIV-infected patients was 37/376 (9.8%) using T primers and 223/333 (67%) using B primers; prevalence was higher in males (167/237, 70.5% vs 56/96, 58.3%; p = 0.033) and sub-Saharan Africans (22/23, 95.6% vs 201/310, 64.8% in other areas). Discordance was also observed in blood donors: 3.8% prevalence using T primers and 51.4% using B primers (also higher in males: 57% vs 37%, p = 0.056). TTV-DNA was detected in PBMC in 20/23 (87%) TTV-positive sera. Two-thirds of the serum samples remained positive over a 2-year follow-up period. CONCLUSION: TTV-DNA prevalence is higher when detected with primers derived from the UTR region and was highest in male and HIV-infected sub-Saharan Africans. TTV-DNA is frequently isolated in PBMC and chronic infection is common.

Adult↗

[Extracorporeal membrane oxygenation (ECMO) as cardiac assist device in pediatrics].

Extracorporeal membrane oxygenation (ECMO) is a common treatment for severe respiratory failure. However, ECMO can also be used as cardiac support. ECMO is the most common mechanical circulatory assist device in pediatrics, followed by pulsatile ventricular assist devices. Advantages are the simultaneous support of respiratory and hemodynamic functions and the fast bedside availability. The major disadvantage is the limited duration of therapy. The transthoracic connection of ECMO after cardiac failure in cardiothoracic surgery in childhood allows a controlled weaning on the intensive care unit. Weaning from ECMO is successful in about 45 to 80 % in cases of circulatory support; survival rates depend on patient selection, treatment protocol and indication. Bleeding problems, thromboembolic complications, hemolysis, infections, renal and neurologic complications are major problems.

Cardiac Surgical Procedures↗

Local perivascular application of low amounts of a plasmid encoding for vascular endothelial growth factor (VEGF165) is efficient for therapeutic angiogenesis in pigs.

Clinical trials have demonstrated therapeutic benefit in inducing angiogenesis in chronic occlusive arterial disease. The route of application mostly used was the intramuscular injection of high dosages of plasmid. Therefore, a local perivascular application of low amounts of vascular endothelial growth factor (VEGF) plasmid was used in an interventional occlusion model, and the effect of VEGF on coronary and peripheral occlusions compared in the same animal model. Coronary and peripheral arteries were chronically occluded in Pietrain pigs using a non-surgical, interventional approach. Adventitial delivery of the DNA for VEGF was performed with a needle injection catheter. The DNA was applied as lipoplexes using the novel cationic liposomes DOCSPER. Optimized transfer conditions were used. Angiography, polymerase chain reaction (PCR), reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry were undertaken within a follow-up period of 6 months. Expression of the transfected VEGF gene was observed at 1 and 3 weeks following application. The DNA was detected up to 5 months following application. Around occluded coronary arteries, there was formation of new collaterals and arterial prolongation, whereas surrounding occluded peripheral arteries there was no collateralization but development of new arterial branches was seen. Results demonstrate that the response to VEGF is also sufficient, when minimal amounts of plasmid encoding for VEGF are applied locally into the perivasculature allowing for more safety of this therapy. Comparison of treatment of chronic coronary and peripheral arterial disease revealed differences in angiogenesis following VEGF application during a total follow-up period of almost 6 months which may be related to their different developmental origins. This may have important implications for developing future therapeutic strategies using VEGF in different vessels.

Animals↗

Fulminant ischaemic colitis with atypical clinical features complicating sickle cell disease.

Clinically significant ischaemic bowel injury is an exceedingly rare complication of sickle cell disease. It manifests as acute surgical abdomen and may respond to conservative treatment. An unusual fatal case of ischaemic colitis with minimal abdominal findings in a young male during a sickle cell vaso-occlusive pain crisis is described. This case demonstrates that an acute surgical abdomen should be considered in such patients who fail to respond to conservative management as untreated this condition may be fatal.

Abdomen, Acute↗

[Lipomatous tumors of soft tissues in the extremities and the waist in adults].

Lipomatous tumors of the upper and lower extremities in adults are frequent and lipoma is the most common soft tissue tumor. Most have a typical appearance at imaging but presentation and management may vary based on the exact histological subtype. The purpose of this paper is to review the main clinical, radiological and histological features of the different benign and malignant lipomatous tumors.

Abdomen↗

Small angle neutron scattering and gel filtration analyses of neutrophil NADPH oxidase cytosolic factors highlight the role of the C-terminal end of p47phox in the association with p40phox.

The NADPH oxidase of phagocytic cells is regulated by the cytosolic factors p47(phox), p67(phox), and p40(phox) as well as by the Rac1-Rho-GDI heterodimer. The regulation is a consequence of protein-protein interactions involving a variety of protein domains that are well characterized in signal transduction. We have studied the behavior of the NADPH oxidase cytosolic factors in solution using small angle neutron scattering and gel filtration. p47(phox), two truncated forms of p47(phox), namely, p47(phox) without its C-terminal end (residues 1-358) and p47(phox) without its N-terminal end (residues 147-390), and p40(phox) were found to be monomeric in solution. The dimeric form of p67(phox) previously observed by gel filtration experiments was confirmed. Our small angle neutron scattering experiments show that p40(phox) binds to the full-length p47(phox) in solution in the absence of phosphorylation. We demonstrated that the C-terminal end of p47(phox) is essential in this interaction. From the comparison of the presence or absence of interaction with various truncated forms of the proteins, we confirmed that the SH3 domain of p40(phox) interacts with the C-terminal proline rich region of p47(phox). The radii of gyration observed for p47(phox) and the truncated forms of p47(phox) (without the C-terminal end or without the N-terminal end) show that all these molecules are elongated and that the N-terminal end of p47(phox) is globular. These results suggest that the role of amphiphiles such as SDS or arachidonic acid or of p47(phox) phosphorylation in the elicitation of NADPH oxidase activation could be to disrupt the p40(phox)-p47(phox) complex rather than to break an intramolecular interaction in p47(phox).

Chromatography, Gel↗

Event-related changes in neuromagnetic activity associated with syncopation and synchronization timing tasks.

For low rhythmic rates (1.0 to approximately 2.0 Hz), subjects are able to successfully coordinate finger flexion with an external metronome in either a syncopated (between the beats) or synchronized (on each beat) fashion. Beyond this rate, however, syncopation becomes unstable and subjects spontaneously switch to synchronization to maintain a 1:1 stimulus/response relationship. We used a whole-head magnetometer to investigate the spatiotemporal dynamics of neuromagnetic activity (MEG) associated with both coordinative patterns at eight different rates spanning the range 1.0-2.75 Hz. Timing changes in the event-related fields accompanied transitions from syncopation to synchronization and followed the placement of the motor response within each stimulus/response cycle. Decomposition of event-related fields into component auditory and motor brain responses revealed that the amplitude of the former decreased with increasing coordination rate whereas the motor contribution remained approximately constant across all rates. Such an interaction may contribute to changes in auditory-motor integration that cause syncopation to become unstable. Examination of event-related changes in high frequency bands revealed that MEG signal power in the beta band (15-30 Hz) was significantly lower during syncopated coordination in sensors covering the contralateral sensorimotor area suggesting a dependence of beta rhythm amplitude on task difficulty. Suppression of beta rhythms was also stronger during synchronization preceded by syncopation, e.g., after subjects had switched, when compared with a control condition in which subjects synchronized throughout the entire range of rates.

Acoustic Stimulation↗

[Coronary arterial stent implantation proceeding noncardiac surgical procedures. Is it safe; can we recommend it?].

Patients undergoing non-cardiac surgical procedures who carry coronary artery stents have to be classified as high risk patients. Perioperative myocardial infarction and severe bleeding are possible. Therefore, anaesthetic management directed by invasive monitoring, ECG ST analysis, transesophageal echocardiography and referral to an intensive care unit are absolutely justified. The urgency of the surgical procedure, perioperative risk and an antiplatelet regimen have to be discussed with the patient and the surgeon in advance. In the case of cardiac complications, rapid therapy by an interventional cardiologist must be available.

Anesthesia↗

Neuromagnetic activity in alpha and beta bands reflect learning-induced increases in coordinative stability.

OBJECTIVE: To investigate how learning induced increases in stability on a syncopation task are manifest in the dynamics of cortical activity. METHOD: Magnetoencephalography was recorded from 143 sensors (CTF Systems, Inc). A pre-training procedure determined the critical frequency (F(c)) for each subject (n=4). Subjects either syncopated or synchronized to a metronome that increased in frequency from 1.2 to 3.0 Hz in 0.2 Hz steps. The F(c) was the point at which subjects spontaneously switched from syncopation to synchronization. Subjects then underwent 100 training trials (with feedback) at F(c). Following the learning phase the pre-training procedure was repeated. RESULTS: An increase in the F(c) occurred indicating that practice improved the stability of syncopation. The transition delay was also observed in the phase of the time-averaged signal in sensors over the contralateral sensorimotor area and in power analysis in the 8-12 Hz and 18-24 Hz frequency bands. Initially, reduced power was observed bilaterally during syncopation compared to synchronization. Following training, these differences were reduced or eliminated. CONCLUSION: Pre-training power differences can be explained by the greater difficulty of the syncopation task. The reduction in power differences following training suggests that at the cortical level, syncopation became more similar to synchronization possibly reflecting a decrease in task and/or attention demands.

Acoustic Stimulation↗

HIV protease inhibitors attenuate adherence of Candida albicans to epithelial cells in vitro.

Oropharyngeal candidiasis is one of the first and most commonly reported opportunistic infections of untreated AIDS patients. With the introduction of the new antiviral HAART therapy, including HIV protease inhibitors, this mucocutaneous infection is nowadays only rarely observed in treated patients. It was recently shown that HIV protease inhibitors have a direct attenuating effect on Candida albicans secreted aspartic proteinases (Saps), an investigation prompted by the fact that both Sap and HIV protease belong to the superfamily of aspartic proteinases and by the observation that mucocutaneous infections sometimes resolve even in the absence of an immunological improvement of the host. As these Saps are important fungal virulence factors and play a key role in adhesion to human epithelial cells we tried to assess the effect of the HIV protease inhibitors Ritonavir, Indinavir and Saquinavir on fungal adhesion to these cells. The effect on phagocytosis by polymorphonuclear leukocytes was also assessed. Ritonavir was found to be the most potent inhibitor of fungal adhesion. A dose-dependent inhibition of adhesion to epithelial cells was found already at 0.8 microM and was significant at 4 microM or higher, at 500 microM the inhibition was about 55%. Indinavir and Saquinavir inhibited significantly at 4 microM or 20 microM, respectively; at 500 microM the inhibition was 30% or 50%. In contrast, no protease inhibitor was able to modulate phagocytosis of Candida by polymorphonuclear leukocytes. In conclusion, inhibition of Saps by HIV protease inhibitors may directly help to ease the resolution of mucosal candidiasis. In future, derivatives of HIV protease inhibitors, being more specific for the fungal Saps, may form an alternative in the treatment of mucosal candidiasis insensitive to currently available antimycotics.

Aspartic Acid Endopeptidases↗