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Biomedical subjects

A Fluet

Publications and source records attributed to A Fluet.

5 recordsLinked to original sources
PubMed1998

In vivo aggregation of beta-amyloid peptide variants.

Transgenic Caenorhabditis elegans animals have been engineered to express wild-type and single-amino acid variants of a long form of human beta-amyloid peptide (A beta 1-42). These animals express high levels (approximately 300 ng of A beta/mg of total protein) of apparently full-length peptide, as determined by quantitative immunoblot. Expression of wild-type A beta in these animals leads to rapid production of amyloid deposits reactive with Congo red and thioflavin S. This model system has been used to examine the effect of Leu17Pro, Leu17Val, Ala30Pro, Met35Cys, and Met35Leu substitutions on the in vivo production of amyloid deposits. We find that the Leu17Pro and Met35Cys substitutions completely block the formation of thioflavin S-reactive deposits, implicating these as key residues for in vivo amyloid formation. We have also constructed transgenic strains expressing a novel A beta variant, the single-chain dimer. Animals expressing high levels of this variant also fail to produce thioflavin S-reactive deposits.

Amino Acid Sequence↗
PubMed1990

Sexual dimorphisms in the vocal control system of a teleost fish: ultrastructure of neuromuscular junctions.

In the sound-generating fish, Porichthys notatus, large, nest-guarding 'Type I' males use their swimbladder 'drumming' muscles to produce acoustic communication signals. Females and another group of smaller sexually mature males ('Type II') have not been observed to produce sounds. Electron microscopy was used to compare the morphology of the neuromuscular junctions in vocalizing Type I males to those of Type II males and females. Significant differences were seen in synaptic vesicle density, terminal size, degree of terminal invagination below the muscle fiber surface, number of Schwann cell processes along the non-synaptic boundary of boutons, and the number of boutons per innervation site.

Air Sacs↗