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Biomedical subjects

A Fleck

Publications and source records attributed to A Fleck.

79 records · Page 5Linked to original sources
PubMed1965

Protein synthesis in rat liver: influence of amino acids in diet on microsomes and polysomes.

Rats were killed 1 hour after having been fed by stomach tube with amino acid mixtures that were either nutritionally complete or lacked tryptophan. Microsomes isolated from the livers of animals fed the incomplete mixture showed a reduced capacity to incorporate (14)C-leucine in vitro, and polysomes prepared from the same livers showed an increased proportion of monosomes and disomes. Prior treatment with actinomycin D did not prevent these differences in response to the two amino acid mixtures, an indication that synthesis of messenger RNA may not be involved in the cell mechanism which recognizes differences in amino acid pattern.

Amino Acids↗
PubMedDate not supplied

Randomized clinical assay for hepatic grafts preservation with University of Wisconsin or histidine-tryptophan-ketoglutarate solutions in liver transplantation.

University of Wisconsin (UW) solution has been the standard for preservation of liver transplantation grafts since 1989. However, some studies demonstrated that histidine-tryptophan-ketoglutarate (HTK) solution is also effective. The purpose of this study was to compare the efficacy of both solutions in liver transplantation. From January 2003 to August 2004 the livers of deceased donors were randomized into HTK and UW groups. The 102 studied patients included 65 (63.7%) in the UW group and 37 (36.3%) in the HTK group. Sex, race, hemodynamic state, use of adrenergic drugs, and presence of steatosis in the donor were similarly distributed in the two groups (P > .05). The mean age of the donors was 38.1 years (SD +/-14.4) in the UW group and 44.6 years (SD +/-14.2) in the HTK cohort (P = .036). Sex, race, age, etiology of the cirrhosis, retransplant, acute liver failure, portal thrombosis, and Child-Pugh and MELD scores in the recipients were similarly distributed in the two recipient samples (P > .05). Among 89 patients who completed 4 months of follow-up, the HTK group included eight cases (25.8%) of biliary complications versus five cases (8.6%) in the UW group (P = .033; OR = 2.0 95% CI = 1.2-3.5). The incidence of graft dysfunction was 2.8% in the HTK group and 9.4% in the UW group (P = .15). In conclusion, UW and HTK solutions were equally effective for the preservation of the hepatic graft. The routine use of HTK solution can reduce the costs of liver transplantation.

Adenosine↗
PubMedDate not supplied

Albumin catabolic rate and protein-energy depletion.

Severe malnutrition was induced in New Zealand White rabbits by restricting dietary nitrogen and energy to 9.1 and 57.4% of normal intake, respectively. Albumin distribution and metabolism were then determined in these and in control animals. The fractional catabolic rate was reduced by 33.7% in the depleted animals, but there were no changes in the other distribution rate constants. In particular, we were unable to demonstrate a significant change in the relative distribution of albumin between the intravascular and extravascular spaces. In addition, albumin concentration in the depleted group only fell toward the end of the experiment. We conclude that, with acute severe malnutrition, plasma albumin concentration is maintained until late and is therefore not a good nutritional marker. Also, maintenance of intravascular albumin concentration is not at the expense of extravascular albumin.

Animals↗