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Biomedical subjects

A Fiori

Publications and source records attributed to A Fiori.

67 records · Page 4Linked to original sources
PubMedDate not supplied

The ABO(H) paradoxical and aberrant secretion in human saliva.

The paradoxical secretion and aberrant secretion of the ABH substances have been studied in the saliva of 251 and 283 subjects, respectively. A standardized hemagglutination-inhibition method was used and the results were scored by an Inhibition Index. Frequencies of 4.3% for paradoxical secretion and of 5.3% for aberrant secretion were observed. Gel chromatography on Sephadex G-25 showed that only the paradoxical secretors have in their saliva the oligosaccharides which inhibit nonspecifically the anti-A, anti-B and anti-H reagents.

ABO Blood-Group System↗
PubMedDate not supplied

Evaluation of cocaine use during pregnancy through toxicological analysis of hair.

Toxicological analysis of hair was used to detect cocaine use in a group of 615 pregnant women. Hair samples were washed, enzymatically digested, and analyzed by radioimmunoassay for cocaine. Positive results were confirmed by gas chromatography-mass spectrometry after a solid-phase extraction. Benzoylecgonine (BZE) and cocaine were detected after derivatization with pentafluoropropionic-anhydride-pentafluoropropanol. Deuterated cocaine and BZE were used as internal standards. This study demonstrated a mean frequency of 1.9% cases positive for cocaine in all the hair samples examined. The positive rate was 6% among women admitted for spontaneous abortion (66 cases), and the positive rate was 1.4% for those who carried to full term (549 cases). These data underline the usefulness of hair analysis for the diagnosis of drug abuse and demonstrate that there is significant use of cocaine in the Italian population.

Abortion, Spontaneous↗
PubMedDate not supplied

[Efficacy of GH-releasing hormone and biosynthetic GH in a group of children with growth hormone deficiency].

Eight GH-deficient children were treated with GHRH (1-44), once daily s.c. for 6-18 months. At the 6th month of therapy, 3 patients showed a catch-up growth ("responders"), while the remaining 5 did not ("non-responders"). In all patients treatment was discontinued after 6-18 months, when its effect on growth rate failed. After a wash out period of at least 6 months, patients were submitted to biosynthetic GH therapy. After 6 months of GH treatment a significant catch-up growth was found in both responder and non-responder children. GHRH therapy is found to be less effective than GH treatment. Other methods of GHRH administration are worth investigating.

Child↗
PubMedDate not supplied

[Lymphoproliferation stimulating activity in serum].

To characterize the serum growth promoting activity estimated by means of bioassay (Thymidine Activity), the gel filtration chromatographic profile of sera obtained from 10 normal adults and from 10 normal infants was performed. For each serum fraction we evaluated the capacity to stimulate the tritiated thymidine (3H-TdR) uptake into lectin-activated lymphocytes. This capacity was expressed as percent increase in 3H-TdR uptake in presence and in absence of the optimal dose (1.25%) of the fraction. The highest percent increase in 3H-TdR uptake was obtained in the same three fractions (1500-2500 Kd, 28-42 Kd, 1.3-1.9 Kd) in the sera of adults as well as infants. The values observed in infants were higher than in adults, suggesting that the serum growth-promoting activity could be higher in rapidly growing subjects.

Adult↗
PubMedDate not supplied

[In vitro effects of GH and IGF-1 on natural cytostatic activity].

We previously reported that natural cytotoxicity, mediated by Natural Killer (NK) cells, is low in GH-deficient children and increases up to normal levels after a long-term treatment. In order to investigate the role of GH and IGF1 on the restoration of this immune function, we studied the in vitro effect of increasing doses of biosynthetic GH and IGF1 on NK activity of GH-deficient children. No variations of NK activity were observed after incubation of GH-deficient children's cells in the presence of GH as well as IGF1. Our data suggest the effect of GH and IGF1 on NK activity not to be direct, but mediated by unidentified factors.

Child↗