Biomedical subjects

A Fenster

Publications and source records attributed to A Fenster.

At least 19 recordsLinked to original sources

PubMed1999

Application of dynamic computed tomography for measurements of local aortic elastic modulus.

A novel computed tomographic (CT) technique used for the instantaneous measurement of the dynamic elastic modulus of intact excised porcine aortic vessels subjected to physiological pressure waveforms is described. This system was comprised of a high resolution X-ray image intensifier based computed tomographic system with limiting spatial resolution of 3.2 mm-1 (for a 40 mm field of view) and a computer-controlled flow simulator. Utilising cardiac gating and computer control, a time-resolved sequence of 1 mm thick axial tomographic slices was obtained for porcine aortic specimens during one simulated cardiac cycle. With an image acquisition sampling interval of 16.5 ms, the time sequences of CT slices were able to quantify the expansion and contraction of the aortic wall during each phase of the cardiac cycle. Through superficial tagging of the adventitial surface of the specimens with wire markers, measurement of wall strain in specific circumferential sectors and subsequent calculations of localised dynamic elastic modulus were possible. The precision of circumferential measurements made from the CT images utilising a cluster-growing segmentation technique was approximately +/- 0.25 mm and allowed determination of the dynamic elastic modulus E(dyn) with a precision of +/- 8 kPa. Dynamic elastic modulus was resolved as a function of the harmonics of the physiological pressure waveform and as a function of the angular position around the vessel circumference. Application of this dynamic CT (DCT) technique to seven porcine thoracic aortic specimens produced a circumferential average (over all frequency components) E(dyn) of 373 +/- 29 kPa. This value was not statistically different (p < 0.05) from the values of 430 +/- 77 and 390 +/- 47 kPa obtained by uniaxial tensile testing and volumetric measurements respectively.

Animals↗

PubMed1999

Quantitative angiographic blood-flow measurement using pulsed intra-arterial injection.

A technique for quantitative blood-flow measurement using a novel pulsed injection of radiographic contrast agent is reported. A pressurized source of contrast agent is interrupted by a rotary valve at rates ranging from 1 to 30 Hz, producing well-defined boli at the end of a catheter. The position of these boli can be recorded by a digital radiographic system and analyzed by one of several previously reported techniques, to produce quantitative measurements of blood velocity and flow rate throughout the cardiac cycle. The contrast-agent flow wave form produced by the pulsed injector has been measured with an electromagnetic flow meter, for driving pressures ranging from 600 to 1500 kPa. Excellent modulation of the contrast agent is observed for injection frequencies up to 20 Hz, through catheters up to 100 cm in length. Preliminary in vitro angiographic flow measurements have been performed using an x-ray image intensifier, coupled to a linear photodiode array as the digital detector. Both constant flow and pulsatile human blood-flow wave forms were simulated within a 6.4-mm-diam straight tube and monitored with an electromagnetic flow meter. These experiments indicate that the pulsed injector can be used to provide estimates of arterial blood flow over the entire cardiac cycle (including reverse flow), to within about +/-11%, following injection of less than 10 ml of iodinated contrast agent.

Angiography, Digital Subtraction↗

PubMed1999

A new flow model for Doppler ultrasound study of prosthetic heart valves.

BACKGROUND AND AIM OF THE STUDY: Steady and pulsatile flow models used to assess the hydrodynamic aspects of prosthetic heart valves are generally made of Plexiglas and Lucite tubing. They often allow continuous-wave and pulsed-wave Doppler ultrasound velocity measurements to be made parallel to the flow, but cannot be used as such for ultrasound scanning of valve inflow and outflow velocities because of ultrasonic reverberation and refraction by the tubing. The aim of the study was to develop a new flow model which allowed ultrasonic scanning of the prosthetic valve flow for three-dimensional (3D) reconstruction of color Doppler flow distributions. METHODS: The flow model, designed with left ventricular and aortic chambers composed of agar gel which mimics the ultrasound characteristics of biological tissues, was developed and tested for comparative in vitro hydrodynamic and Doppler ultrasonic studies of aortic prosthetic valves. An electromagnetic flowmeter and a pressure monitor provided the flow and pressure signals for the hydrodynamic tests. The Doppler ultrasonic evaluation was performed with an Ultramark 9 HDI ultrasound system and a 3D ultrasound imaging system. The model was designed to enable assessment of prosthetic valve performance by pulsed-wave and continuous-wave Doppler velocity measurements, as well as by 3D color Doppler velocity measurements obtained by ultrasonic scanning of the left ventricle or aortic chamber with an ultrasound probe mounted on a motorized translation assembly. RESULTS: The study results showed that this new flow model can provide 3D color Doppler velocity distributions as well as accurate comparisons of hydrodynamic parameters of mechanical and bioprosthetic heart valves derived from Doppler and catheter measurements, both under steady and pulsatile flow conditions. CONCLUSION: This new flow model can be used to evaluate the usefulness of hydrodynamic parameters for the assessment of prosthetic heart valves using both conventional Doppler echocardiography, as currently used in patients, and 3D color Doppler ultrasonic imaging.

Blood Flow Velocity↗

PubMed1998

Three-dimensional ultrasound imaging of the vasculature.

With conventional ultrasonography, the diagnostician must view a series of two-dimensional images in order to form a mental impression of the three-dimensional anatomy, an efficient and time consuming practice prone to operator variability, which may cause variable or even incorrect diagnoses. Also, a conventional two-dimensional ultrasound image represents a thin slice of the patients anatomy at a single location and orientation, which is difficult to reproduce at a later time. These factors make conventional ultrasonography non-optimal for prospective or follow-up studies. Our efforts have focused on overcoming these deficiencies by developing three-dimensional ultrasound imaging techniques that are capable of acquiring B-mode, colour Doppler and power Doppler images of the vasculature, by using a conventional ultrasound system to acquire a series of two-dimensional images and then mathematically reconstructing them into a single three-dimensional image, which may then be viewed interactively on an inexpensive desktop computer. We report here on two approaches: (1) free-hand scanning, in which a magnetic positioning device is attached to the ultrasound transducer to record the position and orientation of each two-dimensional image needed for the three-dimensional image reconstruction; and (2) mechanical scanning, in which a motor-driven assembly is used to translate the transducer linearly across the neck, yielding a set of uniformly-spaced parallel two-dimensional images.

Algorithms↗

PubMed1998

Analysis of linear, area and volume distortion in 3D ultrasound imaging.

We have developed a three-dimensional (3D) ultrasound imaging system that uses a side-firing probe, axially rotated under computer control, to acquire a series of 2D images, from which the 3D image is reconstructed. For an undistorted reconstruction, the inner radius R0 of the 2D images and the total scanning angle theta must be known accurately. Here, we describe (a) a theoretical analysis of the relative distortion in image shape, length, area, and volume due to an error delta R in R0 or delta theta in theta; (b) measurements of these in simulated and real 3D images; and (c) a method to calibrate R0, theta, and image scale accurately. Theoretically, all four relative distortions vary as P delta R/R + Q delta theta/theta, where magnitude of P < or = 1, magnitude of Q < or = 1, and R is the average distance of the object from the axis. In every case, the simple theoretical formulas for P and Q agree with image measurements to within the measurement uncertainty.

Algorithms↗

PubMed1998

Intra- and inter-observer variability and reliability of prostate volume measurement via two-dimensional and three-dimensional ultrasound imaging.

We describe the results of a study to evaluate the intra- and inter-observer variability and reliability of prostate volume measurements made from transrectal ultrasound (TRUS) images, using either the (optimal) height-width-length (HWL) method (V = pi/6 HWL) with two-dimensional (2D) TRUS images (obtained as cross-sections of three-dimensional [3D] TRUS images) or manual planimetry of 3D TRUS images (the 3D US method). In this study, eight observers measured 15 prostate images, twice via each method, and an analysis of variance (ANOVA) was performed. This analysis shows that, with the 3D US method, intra-observer prostate volume estimates have 5.1% variability and 99% reliability, and inter-observer estimates have 11.4% variability and 96% reliability. With the HWL method, intra-observer estimates have 15.5% variability and 93% reliability, and inter-observer estimates have 21.9% variability and 87% reliability. Thus, in vivo prostate volume estimates from manual planimetry of 3D TRUS images have much lower variability and higher reliability than HWL estimates from 2D TRUS images.

Analysis of Variance↗

PubMed1998

In vitro evaluation of multiple arterial stenoses using three-dimensional power Doppler angiography.

PURPOSE: The study was done to improve quantification of multiple arterial stenoses and to investigate a new imaging technique for lower limb arteries. Three-dimensional power Doppler angiography was used to quantify in vitro arterial stenoses. METHODS: We built two types of artery phantoms containing multiple stenoses. One used stenotic porcine arteries, and the other was designed to control the proximal and distal stenoses while we assessed central stenosis of a wall-less agar lumen. Three-dimensional power Doppler angiograms of the flow lumens were generated at different flow rates under steady and pulsatile flow conditions with a PowerPC 8500 computer-based three-dimensional ultrasound imaging system. This experimental system works off-line, performs three-dimensional acquisition, reconstruction, and display of ultrasound images. Images of flow lumens were compared with the measured B-mode images or the true geometry. RESULTS: This technique produces good three-dimensional angiographic images of the flow lumen, and multiple stenoses do not affect the diagnosis of arterial stenoses. With this technique, the average errors for estimating 80% and 50% area reduction stenoses were -10% and 4%, respectively. CONCLUSIONS: Three-dimensional power Doppler angiography has the potential to quantitatively grade multisegmental stenoses in lower limbs and generate a map for vasculature surgery planning.

Animals↗

PubMed1998

Three-dimensional ophthalmic contact B-scan ultrasonography of the posterior segment.

PURPOSE: A system to produce three-dimensional computer reconstructions of ophthalmic contact B-scan ultrasound was developed and investigated. METHODS: Investigators used ocular phantoms to measure the accuracy and reproducibility of linear, area, and volume measurements. RESULTS: In vitro calibration tests of linear and area measurements demonstrate accurate and reproducible findings throughout the imaged space. Phantom volume tests also show reasonable accuracy and reproducibility. CONCLUSIONS: Three-dimensional ultrasonography is effective in measuring length, area, and volume in an experimental model. The in vitro accuracy and reproducibility of measurements warrants further investigation into the clinical utility of this method in posterior segment tumors and other posterior segment pathology.

Equipment Design↗

PubMed1998

Three-dimensional transrectal ultrasound guided cryoablation for localized prostate cancer in nonsurgical candidates: a feasibility study and report of early results.

PURPOSE: A 3-dimensional (D) transrectal ultrasound imaging system was incorporated into the cryoablation routine for prostate cancer to assess its feasibility and use. The objective was to improve visibility for probe placement and for intraoperative monitoring. MATERIALS AND METHODS: A commercially available transrectal ultrasound unit was coupled with a custom designed software system to construct 3-dimensional prostate images. A total of 52 patients with clinically localized prostate cancer, in whom radiotherapy had failed (45) or who were otherwise judged to be nonsurgical candidates (7) were treated with cryoablation, using 3-D transrectal ultrasound for intraoperative guidance. RESULTS: In all cases the intraoperative 3-D transrectal ultrasound images provided a unique coronal view of the prostate, revealing useful information and facilitating more precise probe placement and treatment monitoring. Early postoperative histological and biochemical results and complication rates compare well with other contemporary series. CONCLUSIONS: Although the precise role of cryoablation in the management of prostate cancer remains unclear and long-term results are pending, incorporation of a 3-D transrectal ultrasound imaging system into the cryoablation routine proved to be feasible and appeared to be a worthwhile effort to facilitate the procedure, and deserves further evaluation.

Cryosurgery↗

PubMed1997

X-ray imaging technique for in vitro tissue composition measurements using saline/iodine displacement: technique optimization.

An in vitro radiographic technique which uses saline/iodine displacement has been developed to study the thickness of bone-equivalent and soft-tissue-equivalent materials within atherosclerotic plaques in arterial specimens which have been cut open longitudinally and laid flat. Results concerning the optimization of the imaging parameters are presented and discussed. The technique consists of imaging arterial specimens under two different conditions: (1) when it is immersed in an isotonic saline solution, to estimate the calcium content, and (2) when it is immersed in a concentrated iodine solution, to estimate the total thickness of the specimen. Calibration step wedges made out of bone-mimicking and soft-tissue-mimicking materials are imaged simultaneously to generate calibration curves which are used to convert the radiographs into bone-equivalent and soft-tissue-equivalent thickness images. The optimal spectral parameters were determined to be 45 and 100 kVp for the saline and the iodine images, respectively, with a significant amount of added filtration for both images. Inherent systematic inaccuracies due to (1) the nonidealities due to linear attenuation coefficient mismatch between tissue and calibration materials and (2) beam hardening due to heel effect are determined theoretically, and can be used to correct a set of bone-equivalent and the soft-tissue-equivalent images to within +/- 6 microns with an ideal, noise-free imaging system.

Angiography↗

PubMed1997

Tomographic imaging of the angular-dependent coherent-scatter cross section.

A new special-purpose computed tomographic (CT) imaging system is described which produces images based on measurements of the low-angle (0-10 degrees) x-ray diffraction properties of an object. Low-angle scatter in the diagnostic x-ray energy range is dominated by coherent scatter, and the system uses first-generation CT geometry to acquire a diffraction pattern for each pencil beam. The patterns are used to reconstruct a series of images which represent the coherent-scatter intensity at a series of scatter angles. To demonstrate the potential of coherent-scatter CT (CSCT), the scanner has been built and used to image a phantom consisting of a water-filled Lucite cylinder containing rods of polyethylene, Lucite, polycarbonate, and nylon. In this paper, the system is described and a sequence of CSCT images of this phantom is shown. Coherent-scatter cross sections of these materials are generated for each pixel from this sequence of images and compared with cross sections measured separately. The resulting excellent agreement shows that the angular-dependent coherent-scatter cross section can be accurately imaged in a tomographic slice through an object. These cross sections give material-specific information about the object. The long-term goal of this research is to make measurements of bone-mineral content for every pixel in a tomographic slice.

Bone Density↗

PubMed1997

A real vessel phantom for imaging experimentation.

Vascular phantoms are used to evaluate imaging techniques such as ultrasound (US), CT, and angiography. They are expected to mimic the vasculature, surrounding tissue, and blood, and therefore must meet specific requirements on the mimicking materials, with respect to x-ray attenuation and acoustic properties (velocity, attenuation). In the past, researchers have used a variety of vessel models, including walled (typically latex tube) and wall-less phantoms (obtained by moulding a lumen in a block of agar). These models lacked the exact geometry of human vessels as well as pathologic features such as plaques and calcifications. To overcome these disadvantages, this paper describes a real vessel phantom for US and x-ray studies. The phantom consists of an agar-filled acrylic box containing a formaldehyde fixed section of a real human vessel (obtained at autopsy) cannulated onto two acrylic tubes. This phantom was evaluated by comparing the images obtained with x-ray angiography, CT, and 3-D B-mode US. The images show good overall correlation based on the location of the geometrical features within the phantom, such as lumen, plaques, and calcifications. Discrepancies, artifacts, and difficulties were minor, and are discussed. The use of a real vessel, with its natural geometry and pathology, makes this phantom attractive for evaluation of imaging techniques including projection radiography, CT and US, and for extending its use to MR and US based flow studies.

Angiography↗

PubMed1997

A quantum accounting and detective quantum efficiency analysis for video-based portal imaging.

The quality of images generated with radiographic imaging systems can be degraded if an inadequate number of secondary quanta are used at any stage before production of the final image. A theoretical technique known as a "quantum accounting diagram" (QAD) analysis has been developed recently to predict the detective quantum efficiency (DQE) of an imaging system as a function of spatial frequency based on an analysis of the propagation of quanta. It is used to determine the "quantum sink" stage(s) (stages which degrade the DQE of an imaging system due to quantum noise caused by a finite number of quanta), and to suggest design improvements to maximize image quality. We have used this QAD analysis to evaluate a video-based portal imaging system to determine where changes in design will have the most benefit. The system consists of a thick phosphor layer bonded to a 1 mm thick copper plate which is viewed by a T.V. camera. The imaging system has been modeled as ten cascaded stages, including: (i) conversion of x-ray quanta to light quanta; (ii) collection of light by a lens; (iii) detection of light quanta by a T.V. camera; (iv) the various blurring processes involved with each component of the imaging system; and, (v) addition of noise from the T.V. camera. The theoretical DQE obtained with the QAD analysis is in excellent agreement with the experimental DQE determined from previously published data. It is shown that the DQE is degraded at low spatial frequencies (< 0.25 cycles/mm) by quantum sinks both in the number of detected x rays and the number of detected optical quanta. At higher spatial frequencies, the optical quantum sink becomes the limiting factor in image quality. The secondary quantum sinks can be prevented, up to a spatial frequency of 0.5 cycles/mm, by increasing the overall system gain by a factor of 9 or more, or by improving the modulation transfer function (MTF) of components in the optical chain.

Biophysical Phenomena↗

PubMed1997

X-ray imaging technique for in vitro tissue composition measurements using saline/iodine displacement: experimental verification.

A novel in vitro radiographic technique using saline/iodine displacement, which can be used to study the bone-equivalent and soft-tissue-equivalent thicknesses within vessel walls, was applied to imaging of arterial specimens. Results concerning the accuracy and precision of the bone-equivalent and soft-tissue-equivalent thickness measurements obtained with this technique are reported and discussed. Planar radiographs of a phantom were obtained under two different conditions: (1) when it is immersed in an isotonic saline solution using a 45-kVp spectrum with no added filtration, and (2) when it is immersed in a concentrated iodine solution using a 100-kVp spectrum with 12.5-mm aluminum-added filtration. Calibration step wedges made out of bone-mimicking and soft-tissue-mimicking materials are imaged simultaneously to generate calibration curves that are used to convert the radiographs into bone-equivalent and total-thickness images. A soft-tissue-thickness image is obtained from the subtraction of the bone-equivalent image from the total-thickness image. Thickness measurements obtained from these images yielded average accuracies of +/- 110 microns for both the bone-equivalent and the soft-tissue-equivalent images. The precision (one standard deviation) of the thickness measurements was +/- 60 and +/- 90 microns for the bone-equivalent and the soft-tissue-equivalent images, respectively. In conclusion, since calcified plaque can become as thick as 3-4 mm, the saline/iodine displacement technique has the potential to be a very useful technique for ex vivo studies of the progression of atherosclerosis because of its high accuracy and precision.

Angiography↗

PubMed1996

Evaluation of an automated real-time spectral analysis technique.

An adaptive real-time Doppler peak-frequency tracing algorithm was evaluated in vitro and compared to manual peak-frequency traces. A computer-controlled pump was used to generate physiological flow waveforms in a vasculature-mimicking phantom. Spectral waveforms were obtained on an ATL HDI along with real-time estimates of diagnostic parameters, including maximum systolic, minimum diastolic, time-averaged peak frequencies and pulsatility and resistance indices. The effect of the signal-to-noise ratio on the measured parameters was investigated. The imprecision in the measured parameters was found to depend somewhat on the waveform shape; e.g., the imprecision in PI was 4.1% for a normal renal waveform and 8.5% for a waveform having reverse diastolic flow. The peak frequency envelopes of the same waveform data were traced manually by nine operators, and the resulting diagnostic parameters were compared to ones obtained from automated peak-frequency traces of the same waveform data. The agreement between parameters measured by the automated routine and those measured manually was found to depend somewhat on the waveform shape; e.g., the bias in the PI was 1.3% for a renal waveform lacking diastolic flow, and 12% for a waveform with reverse diastolic flow. The between-observer variations in the manual measurements ranged from 0.8% up to 9.4%. The overall variations associated with the automated traces were found to be smaller than or equal to those of the manual traces.

Algorithms↗

PubMed1996

A Doppler ultrasound clutter phantom.

We describe two variations of a phantom designed to evaluate the wall filters implemented on colour and spectral Doppler instruments. Both variations use an acoustic beam splitter to place the same Doppler sample volume within a motor-driven clutter belt and a flow source, which is either a second belt (dual-belt phantom) or a vascular phantom (belt/vascular phantom). We used the dual-belt phantom to evaluate the effects of the clutter belt velocity, flow belt velocity and clutter-to-flow power ratio on the reported colour Doppler shifts. The results show that the choice of wall filter, as well as the amplitudes and velocities of the clutter and flow components, affect the measured Doppler shifts. Results obtained with the belt/vascular phantom show that colour Doppler shifts due to the moving fluid depend strongly on the clutter velocity and choice of wall filter. However, only a small dependence on Doppler signal strength was observed.

Blood Flow Velocity↗

PubMed1996

A three-dimensional ultrasound prostate imaging system.

We have developed a three-dimensional (3D) transrectal ultrasound imaging system, based on using a motorized 5 MHz transducer assembly, rotated under microcomputer control, to collect a series of 100 two-dimensional (2D) images, digitized by a video frame-grabber. These are then reconstructed into a 3D image on a computer workstation, permitting the prostate anatomy to be visualized in three dimensions, and distance and volume measurements to be performed. The accuracy of the distance measurements was assessed with a string test phantom, and that of the volume measurements with balloons of known sizes. Also, the resolution degradation engendered by the reconstruction algorithm was assessed by comparing the full-width at half-maximum (FWHM) of string cross-sectional images in the 3D image to their 2D counterparts. The results show that distance and volume measurements are both accurate to about +/- 1%, and that the reconstruction algorithm increases the mean FWHM by 8 +/- 3% axially and 3 +/- 3% laterally.

Endosonography↗

PubMed1996

Three-dimensional power Doppler imaging: a phantom study to quantify vessel stenosis.

This study investigated whether three-dimensional (3D) power Doppler imaging can be used to quantify arterial stenosis and its potential as an alternative to x-ray angiography. Three-dimensional power Doppler images of in vitro stenotic vessels were generated under different hemodynamic conditions with a 3D power Doppler imaging system. This system includes: a Macintosh Quadra 840AV computer used to perform 3D imaging acquisition, reconstruction and display; a computer-controlled motor-driven translation assembly used to move the transducer; and an ATL Ultramark 9 HDI ultrasound system. Three vascular- and tissue-mimicking phantoms containing three wall-less stenotic vessels with area reduction of 80%, 50% and 30% were imaged with different flow rates under both steady and pulsatile flow conditions and with different Doppler angles under steady flow condition. With the use of the blood mimic, experimental results demonstrated that power Doppler imaging is nearly independent on flow velocity and Doppler angle. It was also demonstrated that 3D power Doppler imaging can produce nonpulsatile angiographic-like 3D images of the flow field. The stenotic vessels were quantified with an overall accuracy of 8.3% of the vessel area and an overall precision of 7% of the vessel area under the conditions described in this paper. It is believed that 3D power Doppler imaging can be used to quantify arterial stenosis, and in some applications it could be an alternative to x-ray angiography.

Blood Flow Velocity↗