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Biomedical subjects

A F Holloway

Publications and source records attributed to A F Holloway.

At least 19 recordsLinked to original sources

The spectrum and angular distribution of x rays scattered from a water phantom.

To calculate the response of an image receptor to the x rays emerging from a scattering medium, it is necessary to know the x-ray spectrum and intensity as a function of the angle of incidence on the receptor. To permit this calculation for any x-ray spectrum incident on a medium, these functions must be known for monoenergetic x rays. For monoenergetic x rays in the range 20-70 keV we have measured with a high-purity germanium detector the spectrum and intensity of x rays emitted from a water phantom at angles of 0 degree-50 degrees to the direction of the primary beam. The spectrum and intensity of emitted x rays have also been calculated by the Monte Carlo method. At small exit angles, most of the x rays have energies close to the incident energy. As the exit angle increases, the fraction of multiply scattered x rays increases. At very large exit angles, the dominant feature of the spectrum is the peak due to these multiply scattered x rays. For small scattering angles the Monte Carlo calculations are in good agreement with the measurements over the range of energies. For large scattering angeles the scattered photon fluence predicted by Monte Carlo modeling is consistently lower than the measurement in the region just below the full energy peak. The cause of the discrepancies is not fully understood, but cannot be accounted for by Compton broadening alone. An alternate approach to model incoherent scattering is proposed.

Humans

Characterisation of six additional human metallothionein genes.

Human metallothionein (MT) genes are clustered in a locus on chromosome 16, and this report presents the characterisation of the remaining six univestigated members of the family. Nucleotide sequencing in whole or part suggested that four of these genes, MT1I, MT1J, MT1K and MT1L do not encode expressed MT proteins, based on the presence of structural faults or atypical amino acid assignments. On the other hand, the structures of MT1H and MT1X are consistent with these genes being functional and encoding unique type 1 isoforms. The promoters of both genes conferred activity to CAT expression constructs when transfected into HeLa cells, and showed differential responses to inducers MT synthesis. Endogenous MT1H and MT1X genes were expressed at the mRNA level in HeLa cells following cadmium treatment. This work brings the number of functional class 1 and 2 MT genes in the human to eight, and confirms that each encodes structurally unique proteins.

Amino Acid Sequence

Dose and quality control (DQC) in diagnostic radiology.

Dose and quality control in diagnostic radiology can play an important role in reducing x-ray exposure and costs whilst maintaining a high level of imaging quality and diagnostic benefit. It can also become very costly. Current government regulations demand unnecessary accuracy in the measurement and performance of certain parameters of x-ray generators whilst ignoring others which are more important. They totally neglect imaging systems. We urge a more critical approach to the requirements for dose and quality control programs. We propose the exchange of information through a user's club and a less regulatory but equally important role for government.

Calibration

Comparison and variations of the speed of radiographic film.

A study of 50 different batches of film from 20 institutions across Ontario was conducted to measure sensitivities when exposed between intensifying screens and to white light. For films of different types but of the same nominal speed, the x-ray exposures required to produce a net optical density of 1.0 varied by a factor of up to 2.5. For films of the same type from different batches, the required exposure varied by +/- 20%. It was found that sensitivity to white light from the commonly used Wejex sensitometer was not always a good indicator of x-ray sensitivity, and therefore should not be used to compare speeds of different films or films from different batches.

Technology, Radiologic

Speeds of film-screen combinations for radiography.

Values of Ex, the exposure required to yield a net optical density of 1.0, were determined for 12 blue- and nine green-sensitive film types used with 11 blue- and eight green-emitting screen types respectively. The measurements were made using x-rays generated at 80 kVp and 200 mA, under conditions simulating clinical procedures. To examine reciprocity law failure many of the determinations were repeated under similar conditions but at 15 mA. The values of Ex obtained at 15 mA were 6% to 93% greater than those at 200 mA.

Radiographic Image Enhancement

Performance evaluation of image-intensifier tubes.

A series of image quality measurements were obtained over a two-year period for eight image-intensifier tube fluoroscopic units used in clinical practice. The measurements were in agreement with a radiologist's opinion of the image quality for each tube. The results showed that some systems did not change significantly over the two-year period, while others deteriorated from acceptable to unacceptable image quality during this time. For this reason, it is recommended that measurements be obtained for all diagnostic fluoroscopic equipment at least semiannually to monitor image quality and remedy any changes before diagnostic accuracy is severely affected.

Fluoroscopy

A head holder for operative serial angiography.

An apparatus was developed to combine two functions--a versatile neurosurgical head clamp and a compact film changer for operative serial angiography. The construction costs were +15,000, and provided a programmer, six cassettes, and attachments to position the film changer over much of the skull surface. Exposures are obtained by a mobile x-ray machine.

Cerebral Angiography

Temperature-sensitive mutants of vesicular stomatitis virus: viral RNA synthesis in cells infected with mutants belonging to complementation group I.

The RNA polymerase in cells infected with three group I mutants of vesicular stomatitis virus has been examined. Mouse L cells were incubated at the permissive temperature (30 degrees C) for a few hours after infection to allow the development of secondary transcription. The temperature dependence of the secondary transcription system was determined from the incorporation of labelled uridine, in the presence of cycloheximide, at 30 and at 38 degrees C, the later temperature being non-permissive for viral replication. In cells infected with mutants W14, W28, and G11 at a low multiplicity (20 PFU/cells) secondary transcriptase activity was markedly temperature-sensitive after 3 and 5 h of infection at 30 degrees C. At a high multiplicity of infection (1000 PFU/cell) cells infected with W28 showed considerable RNA synthesis at 38 degrees C after 3 h at 30 degrees C. RNA synthesis was also observed in W28-infected cells in which protein synthesis was allowed to continue after the shift from 30 to 38 degrees C. In the latter two cases the RNA synthesized contained 12-18S species but little or no 30S mRNA.

Cycloheximide

Progeny resulting from complementation between mutants of vesicular stomatitis virus.

The complementation properties of the virus progeny released from cells mixedly infected with mutants of vesicular stomatitis virus belonging to four different complementation groups have been examined. The group IV mutant, tsW16B, was tested in combinations with three group I mutants (tsW4, tsW28, and tsG11), one group II mutant (tsG22), and one group III mutant (tsW29). Virus stocks were grown from isolated plaques appearing on the cell monolayers used to assay the mixed infection yields and tested, in a second series of mixed infections, for their ability to complement each of the two parents. It was found that the virus harvested from each one of the first series of mixed infections contained mutants of both parental types.

Animals

Temperature-sensitive mutants of vesicular stomatitis virus: comparison of the in vitro RNA polymerase defects of group I and group IV mutants.

When tested in vitro, certain temperature-sensitive (ts) mutants of vesicular stomatitis virus (VSV) belonging to complementation groups I and IV appear to have defects in the virion-bound polymerase. To obtain further information concerning the nature of these defects, representative mutants were dissociated by the method of S. Emerson and R. Wagner (1972), and their supernatant (S) and pellet (P) fractions were tested for transcriptase activity when combined with the P and S fractions, respectively, of VSV-HR virions. It was found that the S fractions from group I mutants tsW4, 11, 14, 15, and 28 were defective in transcriptase activity, whereas their P fractions were as active as those of VSV-HR. On the other hand, the P fraction derived from virions of the group IV mutant tsW16B showed reduced activity at 25 C and very little activity at 38 C. These results suggest that our group I mutants, like those examined by D. Hunt and R. Wagner (1974), have a defect in the soluble transcriptase enzyme, whereas mutant tsW16B (group IV) has a defect in a sedimentable component required for transcriptase activity, possibly in the ribonucleoprotein template.

Animals