Biomedical subjects
A E Miles
Publications and source records attributed to A E Miles.
'The autobiography of Dr Lilian Lindsay'.
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Wind of change. II--Medical journals in Britain in 1988.
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The effect of incubation temperature on the maintenance of rat palatal mucosa in organ culture.
The effect of incubation temperature on the behavior of neonatal rat palatal mucosa maintained in a chemically defined medium in organ culture for periods up to 7 days was investigated. Explant survival was optimal at 37 degrees C with increasing mortality at temperatures of 34 degrees C and 30 degrees C. There was a transient increase in the epithelial mitotic activity at all temperatures, but at all time intervals mitotic activity was greatest at 37 degrees C. While the mitotic activity at 37 degrees C after 5 hr in vitro was comparable with previously described in vivo values, it was subsequently increased, only returning to values approximating those at the start of the experiment at 6 days. At 30 degrees and 34 degrees C the epithelial mitotic activity increased more slowly than at 37 degrees C; then it followed a similar pattern with time and after 5 days in vitro had fallen to values approximating initial values. At the cut edges of the explants, the rate of epithelial migration and subsequent keratinization increased with increasing temperature. It is suggested that survival of neonatal rat palatal mucosa is optimal in this organ culture system when maintained at 37 degrees C.
A suggested method of preparation of the large bowel for investigation and surgery.
A safe, effective method of large-bowel preparation is outlined, the emphasis being on a standardized technique for the average patient. The rationale for the use of the technique is briefly described, and there is a further section on the preparation of the bowel in a patient with a colostomy. The importance of a residue-free fluid diet and adequate saline purgation is stressed.
The effect of laser photocoagulation on the gastric mucosa and bleeding gastric lesions: an experimental study.
An argon ion laser beam was tested experimentally with regard to its histological effect on and penetration of the gastric mucosa of the rat and man and its ability to induce haemostasis in traumatically produced gastric lesions and transected small blood vessels. Localized laser photocoagulation for periods of up to 5 minutes at 1 watt produced a coagulative necrosis of the gastric mucosa and half of the muscularis propria, but transmural necrosis required prolonged exposure. Laser phototherapy markedly reduced the bleeding time in experimental ulcers and small blood vessels, provided the superficial blood could be adequately dispersed from the direct beam of the laser.
Functional obstruction of the colon--a case report and review.
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The maintenance of rat palatal mucosa in organ culture.
Palatal mucosa from neonatal rats was maintained under organ culture conditions in a chemically defined medium for periods up to 28 days. The histological state of the cultured palatal mucosa was compared with that of control tissue from growing animals of comparable age. The control tissues showed an increase in epithelial thickness, first noticeable at 17 days. Whilst the general structure of the tissues in organ culture was preserved for the duration of the experiment, some changes in epithelial behavior were evident. There was an increase in epithelial thickness up to 6 days, followed by a reduction in the nucleated cell layer of the epithelium to a thickness comparable with that at the start of the experiment. There was a loss of epithelial glycogen within the first day, with occasional reappearance of patchy and irregular deposits. Whereas the control of epithelial thickness appeared to be restored after 10 days in vitro, disturbances in the maturation of the keratinocytes, manifested as epithelial pearls and dyskeratotic cells, were evident at subsequent stages. Epiboly never occurred. The connective tissue component showed continued development, indicated by an increase in the thickness of collagen fibres. The overall palatal growth seen in vivo did not occur in organ culture. We suggest that the improved maintenance reported is partly the result of explanting tissues in such a way as to minimize trauma, and partly the result of incorporating serum albumin into the chemically defined medium.