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Biomedical subjects

A E Jackson

Publications and source records attributed to A E Jackson.

41 records · Page 3Linked to original sources
PubMedDate not supplied

Ultrastructural localization of human kidney antigens using monoclonal antibodies.

A highly sensitive method of ultrastructural-immunoperoxidase staining was developed for use with monoclonal antibodies which have been raised in this laboratory to a variety of antigens of the human kidney. Because of the susceptibility of the antigens to fixation and processing, a four layer, pre-embedding method of staining was used. Results confirmed and clarified previously reported light microscopy results, indicating that an antigen recognized by the PHM5 antibody was found on the podocyte cell membrane within the glomerulus and was not present within the glomerular basement membrane. The antigen was also present on the extraglomerular endothelial cell membrane. The study also demonstrated the presence of an antigen specific to endothelial cells throughout the renal cortex, and gave further insight into the precise localization of glomerular basement membrane components including fibronectin. The method of staining is now being used together with detailed ultrastructural studies to identify the cells produced from isolated glomeruli in tissue culture.

Antibodies, Monoclonal↗
PubMedDate not supplied

Ultrastructural immunoperoxidase investigations of HCG binding to isolated testicular intertubular cells.

The binding and processing of human chorionic gonadotrophin (HCG) on isolated rat testicular intertubular cells was investigated using an ultrastructural immunoperoxidase technique. Following incubation of isolated cells with HCG at a range of temperatures and time intervals, cells were fixed and the presence of HCG demonstrated using monoclonal and polyclonal antibodies to HCG and its alpha and beta subunits. Results indicated that HCG binds to the surface of the Leydig cells where it is rapidly split into its component subunits. Internalization of the HCG molecule was not demonstrated. The testicular macrophages also bound HCG. In contrast to the Leydig cells they were able to internalize the molecule into lysosomes in the cytoplasm. The complete molecule and the alpha and beta subunits were found within the cells. The study suggests that results of previous studies using mixed populations of isolated cells may be due to the combined effects of the different mechanisms present in the two cell types. It is postulated that Leydig cells bind HCG on the surface and cyclic AMP is activated prior to breakdown and loss of the hormone from the surface of the cells. It is postulated that the macrophages play a role in the breakdown of excess trophic hormone and act in concert with the Leydig cells in the local control of testicular function.

Animals↗