[Mesenteric ossification in pigs].
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Animals↗
Biomedical subjects
A E Friess
Publications and source records attributed to A E Friess.
The sperm acrosome: immunological analysis using specific polyclonal and monoclonal antibodies directed against the outer acrosomal membrane of boar spermatozoa.
An antiserum to the purified porcine outer acrosomal membrane (OAM) was raised in female Balb/c mice and was characterized by means of an indirect ELISA. The hyperimmune serum reacted selectively with the acrosomal cap of the sperm head and showed an extremely good cross reactivity with bull and human spermatozoa when assayed by indirect immunofluorescence. Immunoelectron microscopy using the protein A-gold method further confirmed the specificity of the anti-OAM-antiserum for the OAM. In an effort to identify the OAM antigens recognized by the hyperimmune serum and to analyse the extent of cross reactivity on a molecular level, the SDS-extractable proteins were separated by SDS-PAGE, transblotted and immunoprinted using an 125J-conjugated anti-mouse-antibody. To facilitate functional and structural analysis of distinct OAM-proteins monoclonal antibodies were generated by hybridization of mouse myeloma cells with the splenocytes of female Balb/c mice immunized with the purified OAM. One fusion resulted in about 100 anti-OAM-antibodies secreting hybridoma cultures, of which about 30% showed cross reaction with human and bull spermatozoa. Four stable cell lines were selected for this study secreting antibodies directed against the outer acrosomal membrane of boar spermatozoa. Whereas the polyclonal immune mouse serum stained the entire acrosomal cap, the four hybridoma antibodies generated a patch-work-like immunofluorescence pattern over the acrosome. HPLC-ELISA of the solubilized OAM revealed first information on the nature of the corresponding membrane antigen.
Acrosome↗
The junctions between efferent ductules and epididymal duct in the boar.
Sperm transit through the male excurrent duct system is dependent on complete luminal patency. Since male excurrent ducts are derived embryologically from originally separate structures, the junctions between mesonephric tubules and the mesonephric duct are of major interest with respect to obstructive disorders. Therefore, we investigated the junctions between efferent ductules and epididymal duct in adult boars by means of serial semithin and ultrathin sections. Based on the anastomosing pattern and on the site of transition from low columnar, ciliated epithelium to high columnar, non-ciliated epithelium, three different types of anastomoses were identified, one of which was associated with luminal stenosis and sperm accumulation. The occurrence of end-to-side junctions with the epithelial transition being localized within the lumen of the tributary may thus impede normal sperm transport.
Adult↗