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Biomedical subjects

A E Anderson

Publications and source records attributed to A E Anderson.

84 records · Page 5Linked to original sources
PubMed1968

Selective venting of cigarette smoke in dichotomous ducts and preserved human bronchi.

Mechanically generated cigarette smoke and ambient air were injected into dichotomous ducts and geometrically preserved human bronchi in a fashion simulating typical smoking technique. When the air passages were at ambient temperature, the smoke settled into the lower branches. Smoke which was injected into passages warmed to body temperature rose to the upper branches. The latter selective distribution of the smoke resembled the distribution of centrilobular emphysema in the lungs.

Bronchi↗
PubMedDate not supplied

Self-limited babesiosis in a splenectomized child.

This report describes a self-limited babesia infection in a splenectomized child with hereditary spherocytosis. This is the first report of infectious babesiosis in a child and the second case of babesiosis reported on Cape Cod. The infection manifested itself 6 weeks after a 4-hour exposure to Nantucket. Hemolysis lasted only 4 days and resolved without specific treatment. Parasitemia was documented after resolution of symptoms.

Adolescent↗
PubMedDate not supplied

Input-specific immunolocalization of differentially phosphorylated Kv4.2 in the mouse brain.

Voltage-gated A-type potassium channels such as Kv4.2 regulate generation of action potentials and are localized abundantly in the hippocampus and striatum. Phosphorylation consensus sites for various kinases exist within the sequence of the potassium channel subunit Kv4.2, including consensus sites for extracellular signal-regulated kinase/mitogen activated protein kinase (ERK/MAPK), protein kinase A (PKA), protein kinase C (PKC), and calcium/calmodulin-dependent kinase II (CaMKII), and kinase assays have shown that particular amino acids of the consensus sites are bonafide phosphorylation sites in vitro. We have developed antibodies recognizing Kv4.2 triply phosphorylated at the three ERK sites as well as two antibodies recognizing singly phosphorylated Kv4.2 channels at the PKA sites (one amino-terminal and one carboxy-terminal). In the present study, we report the development of reliable immunohistochemistry protocols to study the localization of these phosphorylated versions of Kv4.2, as well as total Kv4.2 in the mouse brain. A general description of the areas highlighted by these antibodies includes the hippocampus, amygdala, cortex, and cerebellum. Such areas display robust synaptic plasticity and have been implicated in spatial, associative, and motor learning. Interestingly, in the hippocampus, the antibodies to differentially phosphorylated Kv4.2 channels localize to specific afferent pathways, indicating that the Kv4.2 phosphorylation state may be input specific. For example, the stratum lacunosum moleculare, which receives inputs from the entorhinal cortex via the perforant pathway, displays relatively little ERK-phosphorylated Kv4.2 or PKA carboxy-terminal-phosphorylated Kv4.2. However, this same layer is highlighted by antibodies that recognize Kv4.2 that has been phosphorylated by PKA at the amino terminus. Similarly, of the three antibodies tested, the soma of CA3 neurons are primarily recognized by the ERK triply phosphorylated Kv4.2 antibody, and the mossy fiber inputs to CA3 are primarily recognized by the carboxy-terminal PKA-phosphorylated Kv4.2. This differential phosphorylation is particularly interesting in two contexts. First, phosphorylation may be serving as a mechanism for targeting. For example, the amino-terminal PKA phosphorylation may be acting as a tag for a discrete pool of Kv4.2 to enter stratum lacunosum moleculare. Second, as phosphorylation may regulate channel biophysical properties, differential phosphorylation of Kv4.2 in the dendrites of pyramidal neurons may confer unique biophysical properties upon particular dendritic input layers.

Amygdala↗
PubMedDate not supplied

Thickness and composition of nonrespiratory bronchiolar walls in normal and emphysematous lungs with some functional implications.

The thickness and composition of the walls of nonrespiratory bronchioles were quantitatively assessed in groups of normal and emphysematous lungs. In both the groups there was a gradual increase in the thickness of the walls of nonrespiratory bronchiles with increasing caliber, but this change was not proportionate. Smaller bronchioles possessed thicker walls in both the groups. Bronchioles of comparable internal diameter were also found to be slightly thicker than normal in emphysematous specimens at all levels of caliber. Through a system of schematic models, it was shown that the relatively thicker walls of smaller bronchiles in normal and emphysematous lungs would enhance airflow in the proper direction both during expiration and inspiration. The slightly thicker walls of bronchioles in emphysema would result in their closure at substantially higher lung volumes than would occur with the somewhat thinner walled bronchioles of corresponding diameters in normal lungs. Although such thickening might only be focal in emphysema, this would interfere with expiratory airflow and result in air-trapping. This investigation failed to demonstrate differences in the composition in the walls of nonrespiratory bronchioles of normal and emphysematous lungs.

Bronchi↗