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Biomedical subjects

A Dryll

Publications and source records attributed to A Dryll.

At least 91 records · Page 5Linked to original sources
PubMedDate not supplied

[Direct reaction between complement system and cell nuclei (author's transl)].

A direct in vitro reaction between complement system and cell nuclei of human leucocytes and of cryostat sections of rat liver or kidney, has been demonstrated by an indirect immunofluorescence technique. The first step of this reaction involves a fixation of C1q to the nuclear DNA as shown by the peripheral distribution of the fluorescence and by the extinction of the fluorescence when the tissue-slices are pretreated by DNase but not by RNase or trypsin. This fixation gives rise to an activation of C1 which can be demonstrated by the capacity of the fixed C1 to induce a fixation of C4 of the same distribution. Although the sequential fixation experiments have not allowed to establish directly the fixation of the following components of the complement system (C2 and C3), the positive results obtained using whole fresh normal human serum as a source of complement and a fluorescent anti-human C3 serum clearly indicate that the activation of the classical pathway by whole cells DNA can go as far as the C3 step. All these results were obtained at physiological pH and molarity: this can suggest a physiopathological meaning for this reaction.

Animals↗
PubMedDate not supplied

Vascular endothelial cell injury in progressive systemic sclerosis and other connective tissue diseases.

The growth inhibition of in vitro vascular endothelial cell cultures by sera from patients with connective tissue diseases was studied. Seven out of 30 sera from patients with progressive systemic sclerosis (PSS) exhibited a cytotoxic effect. The cytotoxic serum factor(s) was not specific to patients with PSS, it was also demonstrated in sera from patients with other connective tissue diseases with vascular symptoms (11 out of 27 sera from patients with a mixed connective tissue disease, MCTD; 5 out of 34 sera from patients with a systemic lupus erythematosus, SLE). The cytotoxic effect was not specific for vascular endothelial cells, it was also found on fibroblast cells cultures. Inhibitor(s) of cell growth in vitro was independent of antinuclear and anticytoplasmic antibodies and appeared as a common feature of all connective tissue diseases with vascular symptoms.

Antibodies↗
PubMedDate not supplied

Relapsing polychondritis - pathogenic role of anti-native collagen type II antibodies. A case report with immunological and pathological studies.

A patient with relapsing polychondritis and Sjogren's syndrome is reported. Diagnosis was confirmed by ear cartilage biopsy. Antibodies against rat costal cartilage were present in the patient's serum. Antinative collagen type II antibodies were detected using a passive hemagglutination technique and the titer grossly correlated with the clinical course. Direct immunofluorescence using the patient's ear cartilage as a target suggested that anti-collagen antibodies might play a major pathogenic role in the course of relapsing polychondritis.

Antibodies↗