Biomedical subjects
A Dormoy
Publications and source records attributed to A Dormoy.
A nucleotide substitution in a Bg1 II site is responsible for the RFLP discrimination between DPw4 and DPa.
In a previous work we showed that the two functionally different specificities DPw4 and DPa could only be differentiated by RFLP analysis using two mutually exclusive fragments (respectively, Bg1 II 5.29 kb for DPw4 and 7.24 kb for DPa). The DP Workshop synthetic analysis localized these fragments in the DPA2 pseudogene region. Our results demonstrate, however, that they are located between the A1 and B1 genes; the Bg1 II restriction site responsible for the 5.29 kb fragment was localized between the first and second exon of the DPB1 gene and inside the 7.24 kb fragment. A single mutation point inside this restriction site is responsible for the absence of the 5.29 kb fragment, changing the specificity attributed by RFLP typing.
Routine HLA-DP typing by RFLP analysis.
HLA-DP typing using the Primed Lymphocyte Test (PLT) is a long and cumbersome technique requiring DP sensitized clones and bulk reagents. We describe here the use of restriction fragment length polymorphism (RFLP) analysis. This method is quicker and was found to be reliable after comparative analysis of the results between PLT and RFLP in 15 local families and 72 core cell lines from the Tenth International Histocompatibility Workshop.
Class II alloantigen expression on leukemic cells.
Class II antigen expression on leukemic cells has been mainly studied using monoclonal antibodies (Mabs). On the other hand, class II polymorphism has been mainly studied using alloantisera. The present study shows that the reactivity of leukemic cells from different lineages with class II Mabs was not always the same as that obtained with alloantisera and that the reactivity varied depending on the leukemic cell-type studied.