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Biomedical subjects

A Dobrina

Publications and source records attributed to A Dobrina.

23 records · Page 2Linked to original sources
PubMed1983

Metabolic properties of freshly isolated bovine endothelial cells.

Studies on endothelial cells metabolism have been limited by the lack of availability of a procedure for obtaining such cells in quantities adequate for direct in vitro analysis. Viable and well-dispersed endothelial cells in high yield have been obtained from the cavernous bodies of bovine penis. A preliminary characterization of the metabolic properties of the isolated cells has concerned the respiratory activity and some aspects of the carbohydrate metabolism. This study points out the following metabolic characteristics of endothelial cells: (1) a low respiration that is inhibited by high glucose concentrations (Crabtree effect); (2) a very high glycolytic activity in aerobic conditions, with a low Pasteur effect; (3) a very high potential activity of the hexose monophosphate shunt relative to the actual flux in the pathway. The biological relevance of these observations is discussed.

Animals↗
PubMed1983

Isolation of metabolically active endothelial cells in high yield from bovine cavernous bodies. A model for functional studies on freshly isolated microvascular endothelial cells.

Work on endothelial cells has been limited by the availability of procedures for obtaining such cells in quantities adequate for direct in vitro analysis. The present paper describes a method for the isolation of endothelial cells from bovine cavernous bodies. A number of cells ranging from 2.5 to 4 X 10(8) per animal has been obtained. The cells were identified as follows 1) presence of the "Weibel and Palade" bodies in the isolated cells, 2) "cobblestone" appearance of cell cultures, and 3) presence of factor VIII, as demonstrated by immunofluorescence assays. The cell viability at the end of the purification procedure was tested 1) by dye-exclusion tests and 2) by metabolic assays. Features of this preparation are 1) the very high yield of viable endothelial cells, 2) the absence of contamination by fibroblasts and smooth muscle cells and a very low contamination by erythrocytes and 3) the fine dispersion of the isolated cells. These properties allow functional and subcellular fractionation studies on freshly isolated endothelial cells of microvascular origin.

Animals↗
PubMedDate not supplied

Complement-endothelial cell interactions: pathophysiological implications.

The function of the endothelial cells can be modulated by humoral factors present in the circulation and in the extravascular fluid, including proteins of the complement system. This review examines the multiple interactions between the complement system and the endothelial cells and their functional consequences on inflammation, coagulation and regulation of vascular tone. The implications of these interactions in the induction and progression of the vascular lesions occurring in atherosclerosis, ischemia/reperfusion and xenotransplantation and the possible therapeutic approaches in terms of complement regulation are also discussed.

Animals↗