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Biomedical subjects

A Dhar

Publications and source records attributed to A Dhar.

At least 91 records · Page 5Linked to original sources
PubMed1985

A simple ELISA for antigiardial antibody using the membrane fraction of the parasite as antigen.

Most existing methods for detecting antigiardia lamblia antibody require the use of cultured Giardia lamblia trophozoites as antigen in immunofluorescent and enzyme-linked immunosorbent assays. However, the maintenance of trophozoite culture systems limits the large-scale use of these antibody detection systems. An antigen extracted from Giardia lamblia by the detergent Triton X-100, when used in an ELISA system, produces specific, objective, quantitative and reproducible results. It is likely that such a test system could be packaged in kit form for large-scale diagnostic and epidemiological application in all parts of the world.

Animals↗
PubMed1983

Impact of lead on nucleic acids and incorporation of labelled amino acid into protein.

The effects of high administration of lead acetate (3.4 mg/100 g of body weight) for a period of 4 days were studied in albino rats. The weights of lead treated rats were not affected while the liver showed enlargement following treatment. Administration of lead reduced the protein content of liver and concentration of DNA decreased to a slight extent. Protein/DNA ratio in liver remained unaltered and this unaltered protein/DNA ratio following lead treatment suggested nonimpairment of cellular protein concentration. The RNA content of liver was increased following treatment and protein/RNA ratio was reduced. RNA per unit amount of DNA was found to be increased and this increased cellular concentration of RNA might result from the degradation of RNA. Lead administration caused a reduction in the incorporation in vivo 14C-leucine into protein of liver. It has been suggested that the diminished incorporation of amino acid into protein following lead treatment might be affected at the translational level of protein biosynthesis.

Amino Acids↗
PubMed1980

Effect of thyroxine on the hepatic glycogen and glucose-6-phosphatase activity of developing rats.

The influence of exogenous thyroxine was studied on the hepatic glycogen content and glucose-6-phosphatase activity of rats of different age groups. The glycogen content and glucose-6-phosphatase activity were found to be decreased in the livers of 5, 15, 30 and 60-day-old rats after thyroxine treatment. In normal rats of 5, 15, 30 and 60-day-old, a gradual rise in both the hepatic glycogen content and glucose-6-phosphatase activity was noted as the age advanced from immature to adult.

Aging↗
PubMed1980

Changes in free amino acid nitrogen concentration and transaminases in lead treated rats.

Intraperitoneal administration of lead acetate for a period of 4 days in albino rats caused an increase of free amino acid nitrogen level in plasma, liver and kidney. There was diminution of glycogen content in both liver and kidney. The activities of plasma alanine-glutamic transaminase and aspartic-glutamic transaminase were increased with reduction in the activities of alanine-glutamic transaminase and aspartic-glutamic transaminases in liver and kidney.

Alanine Transaminase↗
PubMed1979

Effect of lead on certain aspects of protein metabolism.

Administration of lead acetate (3.4 mg/100 g of body weight) by daily intraperitoneal route for 4 days in albino rats caused an increase in size of the liver and kidney. There was diminution of the protein content of the liver. Concentration of DNA decreased to a slight extent and protein/DNA ratio remained unaltered in liver and kidney. There was elevation of the concentration of RNA in both the organs. Protein/RNA ratio was reduced. RNA per unit amount of DNA was found to be increased and the activities of RNase and DNase in both the organs were reduced. There was also diminution of plasma protein level. The possible significance of these changes has been discussed.

Animals↗
PubMed1975

Diverse mechanisms of hepatocellular injuries due to chemicals: evidence in rats administered carbon tetrachloride or dimethylnitrosamine.

Differences in acute hepatotoxicity of carbon tetrachloride (CCl4) and dimethylnitrosamine (DMN) have been tested in normal foetal, newborn and adult rats, foetal, newborn and adult rats pretreated with phenobarbitone and partially hepatectomized adult rats. While CCl4 is non-toxic to the foetal and newborn liver, DMN induces identical necrosis at all ages. Prior dosing with phenobarbitone augments CCl4 toxicity only in the adult and the newborn but the foetus continues to be resistant. Such pretreatment, on the other hand, significantly reduces the effects of DMN on liver in all animals. Partial hepatectomy makes the liver less susceptible to CCl4 and more so to DMN. Such diversities of hepatic response to the two toxins can be accounted for by the levels of the respective toxifying enzymes in the liver cell in different situations.

Age Factors↗
PubMed1975

Increase in serum alpha foetoprotein level in hepatic regeneration of the rat. Effects of age and of magnitude of regenerative activity.

The effects of variable degrees of liver regeneration induced by two-thirds partial hepatectomy or intoxication with different doses of carbon tetrachloride (CCl4) on increased production of alpha foetoprotein (AFP) have been studied in very young (5-6 weeks) and older (15 weeks and more) rats by counter-immunoelectrophoresis (sensitivity down to 250 ng/ml). In the young animal adequate regeneration following two-thirds hepatectomy as well as 100 myl CCl4 successfully induced large increases in serum AFP levels. Smaller doses as well as a large fatal dose of the toxin, all of which resulted in inadequate regenerative activity, failed to excite AFP synthesis and secretion up to detectable levels. The adult animal did not show detectable AFP with any of these procedures. However, necrosis induced in the regenerating adult liver 24 h following partial hepatectomy did result in the detection of small amounts of protein in the serum. It is concluded that in hepatic regeneration of this species, the age of the animal as well as the magnitude of regenerating activity in the liver are critical in the occurrence of high levels of serum AFP.

Age Factors↗