Biomedical subjects

A Dastur

Publications and source records attributed to A Dastur.

2 recordsLinked to original sources

PubMed2001

Promoter analysis in mycobacteria using xyIE reporter assays and its implication in high throughput screening.

Understanding the molecular biology of Mycobacterium tuberculosis has become the focus of recent studies in infectious diseases. Here, we describe a spectrophotometric method for quantitative analysis of promoters in mycobacteria. The assay is a modification of the xyIE reporter assay, which uses an inexpensive substrate, catechol. The distinguishing feature of this protocol is that cells are grown by patching on agar plates, and directly used in the assays. The absorption measurements of the cell suspension at 375 nm in the presence and absence of the substrate are used for obtaining xyIE activity and for normalization of the assay values respectively. These features make the assay system directly amenable for high throughput analyses.

Catechol 2,3-Dioxygenase↗

PubMed1978

Laparoscopy for resolving Müllerian abnormalities.

One hundred thirty-five patients with various müllerian abnormalities underwent laparoscopy. At a glance the precise malformation was diagnosed correctly: 44 patients revealed a complete absence of the müllerian system; 35 were found to have a transverse ridge across the pelvis, the lateral ends of which were well developed; 33 patients had rudimentary uteri; 7, a median müllerian nodule; 5 belonged to the group with the testicular feminization syndrome; 4 were classified as having a bicornuate uterus; 3 had unicornuate uteri; and 3, septate uteri. One rare variety of müllerian abnormality is also described. Laparoscopy was found to be invaluable in the diagnosis of müllerian abnormalities.

Abortion, Habitual↗