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Biomedical subjects

A D Thomas

Publications and source records attributed to A D Thomas.

53 records · Page 3Linked to original sources
PubMed1984

Bender scores and the horse as a distinct item on object assembly of the WISC.

This exploratory study investigated the relationship between two measures of perceptual-motor problems. Ratios derived from the Object Assembly subtest of the WISC with errors greater than 1 SD on the Bender test using Koppitz' scoring system were compared. The sample of 66 white middle-class children was 6 to 11 yr. old. The particular ratio studied was statistically significantly associated with the Bender, whereas ratios of other Object Assembly items were not. The Horse ratio was also significantly associated with the presence of learning problems shown in children's files.

Bender-Gestalt Test↗
PubMed1983

Evaluation of the API 20E and Microbact 24E systems for the identification of Pseudomonas pseudomallei.

One-hundred isolates of Pseudomonas pseudomallei were used to evaluate the API 20E and Microbact 24E rapid identification systems. The API 20E system identified 50% of the isolates using the revised 1979 Manual only, and 63% when referral was made to the computer centre. A higher identification rate (69 and 87%, respectively) was achieved with a longer incubation period of 96 h. The Microbact 24E system identified 84% of the isolates as P. pseudomallei using the revised 1983 Manual, and 100% when referral was made to the computer centre. The Microbact 24E system would appear to be a reliable system for the identification of P. pseudomallei.

Animals↗
PubMed1983

An immunopathologic study of the bovine prepuce.

The prepuces of 83 bulls with macroscopically normal reproductive tracts were obtained at slaughter and microbiological, immunological, and histologic studies were done and the findings were correlated. Some bulls had been vaccinated on several occasions against Campylobacter fetus. Mean concentrations of intrapreputial immunoglobulins (Ig) in 27 bulls were IgG1 - 1.8 +/- 5.2; IgA - 0.16 +/- 0.15; and IgM - 0.24 +/- 0.24 mg/ml. High concentrations of IgG2 in some bulls precluded precise estimation but mean concentration was in excess of 11.0 mg/ml (range 0 to 20+ mg/ml). Differences between these concentrations were significant (P less than 0.005). Mean prevalences of class specific, immunoperoxidase-labeled plasma cells in the preputial dermis of 35 bulls were IgG - 39.0 +/- 9.3; IgA - 16.6 +/- 6.6; and IgM - 2.2 +/- 1.8 labeled cells/100 nuclei (P less than 0.001). The prevalence of IgG labeled cells in the preputial dermis was, however, negatively correlated with the concentration of intrapreputial IgG (IgG1 + IgG2) (r = -0.4; P less than 0.05). Except for an apparently lower intrapreputial Ig concentration in Trichomonas foetus-infected bulls than in negative ones, there were no significant correlations between intrapreputial immunoglobulin concentration, histologic findings, and age, infection, or vaccination status of the bulls.

Animals↗
PubMed1981

Melioidosis with acute meningoencephalomyelitis in a horse.

A case of acute meningoencephalomyelitis caused by infection with Pseudomonas pseudomallei is described. Clinically there was inability to stand, opisthotonus, facial paralysis and nystagmus, rapidly progressing to violent struggling. Gross examination revealed malacia and haemorrhage in the medulla oblongata and adjacent spinal cord. Microscopically there were disseminated focal neutrophilic accumulations in affected areas, perivascular cuffing with mononuclear cells and lymphocytes and marked oedema. Intracellular bacteria were identified in sections stained by the Giemsa method.

Animals↗
PubMed1980

Melioidosis in a galah (Cacatua roseicapilla).

A second case of a natural infection of melioidosis in a native bird is described. Pseudomonas pseudomallei was isolated in pure culture from the liver, spleen and intestinal contents of a galah (Cacatua roseicapilla). The case was characterised by focal granuloma formation, often associated with necrosis, in the brain, lungs, liver, spleen and kidneys.

Animals↗
PubMed1979

Isolation of Pseudomonas pseudomallei from clay layers at defined depths.

Twelve strains of Pseudomonas pseudomallei were isolated from the soil and water of a sheep paddock over a two-year period. The organism was recovered from the clay layer of the soil profile as well as from water that seeps into this layer during the "wet" season. Five isolates were obtained before the commencement of the "wet" season; environmental factors appear to play an important role in the survival of Ps. pseudomallei during the "dry" season. Lower isolation rates were recorded than those indicated by workers in southeast Asia and Iran.

Animals↗
PubMed1979

Brucella suis infection in pregnant cattle.

Six pregnant, Bos taurus cows with stages of gestation ranging from 11 to 33 weeks were each inoculated into the right conjunctival sac with 0.2 ml of a smooth culture of Brucella suis type I containing 27 x 10(6) viable organisms. The 6 cows produced 7 calves of which one single calf and one twin calf were stillborn, the cause of which was not determined. Br. suis was not isolated from any of the cows or calves using either special media or guinea pig inoculation. No abnormality was found in any of the cows or calves at autopsy. Microscopic examination of placentas and tissues from stillborn calves revealed no abnormality. Serologically, 2 weeks after inoculation all 6 cows had positive reactions to the Rose Bengal Test (RBT) and serum agglutination (SAT) titres of 25 iu to 116 iu. However, these reactions disappeared within 11 weeks. Only 2 cows had a complement fixation (CFT) titre which lasted a maximum of 5 weeks and reached a titre of 4/4. Following the anamnestic use of Br. abortus strain 45/20 vaccine on 3 of the cows, positive RBT reactions, SAT titres of 33 iu, 29 iu and 58 iu and CFT titres of 4/16, 1/8 and 3/8 respectively were recorded 6 weeks after vaccination.

Agglutination Tests↗
PubMed1979

Observations on single brucellosis reactors in large herds.

Over a 3 year period specimens were collected from single reacting cattle to the complement fixation test for brucellosis from large herds in north Queensland. Twenty-four such reactors were destroyed for culture, and Brucella abortus was isolated on 3 occasions. The existence of such low levels of Br. abortus in large herds should not be overlooked in the eradication campaign. Explanations were found for 17 of the single reactors, and included introduction of cattle to the herd, exposure to adjoining infected herds or vaccination with Strain 19.

Animals↗
PubMed1978

Melioidosis in a sulphur-crested cockatoo (Cacatua galerita).

A case of melioidosis in a sulphur-crested cockatoo (Cacatua galerita) kept as a pet in Townsville is described. This case was characterised by liver necrosis with both a heterophil and mononuclear cell inflammatory response associated with giant cells. Pseudomonas pseudomallei was isolated by blood plate culture and guinea pig inoculation. This is the first case of melioidosis reported in a native bird in Australia.

Animals↗
PubMed1972

Effect of oxygen on several enzymes involved in the aerobic and anaerobic utilization of glucose in Escherichia coli.

By using the continuous culture technique, the transition from aerobiosis to anaerobiosis and its effect on a number of enzymes has been investigated in Escherichia coli K-12. A decrease in the oxygen partial pressure below 28.0 mm of Hg resulted firstly in an increase of the respiratory enzymes (reduced nicotinamide adenine dinucleotide [NADH] oxidase, 2.53-fold; succinic dehydrogenase, 1.4-fold; cytochrome b(1), 3.91-fold; and cytochrome a(2), 2.45-fold) before the electron transport system gradually collapsed as cytochrome a(2), followed by cytochrome b(1), succinic dehydrogenase, and finally NADH oxidase decreased in activity. The change from respiration to fermentation was initiated well before the oxygen tension reached zero by the increase in levels of fructose diphosphate-aldolase, glucose 6-phosphate, and 6-phosphogluconate dehydrogenases and a decrease in 2-oxoglutarate dehydrogenase. Whem the dissolved oxygen tension reached zero, dry weight and CO(2) formation together with isocitrate dehydrogenase decreased, whereas acid production and phosphofructokinase synthesis started to increase. Enzymatic investigations revealed that the kinetics of the enzyme phosphofructokinase from strict aerobic cultures (6.9 ppm oxygen in solution) was adenosine triphosphate (ATP)-insensitive, whereas the same enzyme from anaerobic cultures was ATP-sensitive. A mechanism is proposed for the change from aerobiosis to anaerobiosis together with the occurring change in glucose regulation.

Adenosine Triphosphate↗