[Cytomorphological changes in Escherichia coli cells exposed to alkylating compounds].
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Biomedical subjects
Publications and source records attributed to A D Shved.
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The use of tungsten trioxide for shadowing nucleic acids is shown to be possible. Approximately 20 mg of tungsten trioxide could be prepared by heating (25 s, 2 V) a coil (4 turns, 1.5 mm i.d.) made of tungsten wire 0.5 mm diameter in a vacuum evaporator at atmospheric pressure. As revealed by electron microscopy, this amount of tungsten trioxide is sufficient for qualitative rotary shadowing phage lambda DNA.
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Using three-functional alkylating compound thiophosphamide complete inactivation of the influenza and herpesviruses of type 1 and 2 was achieved. The method provides full inactivation of DNA- and RNA-containing viruses and preservation of their immunogenic properties.