Biomedical subjects

A D Bedrick

Publications and source records attributed to A D Bedrick.

21 records · Page 2Linked to original sources

PubMed1978

Epidermal growth factor potentiates cortisone-induced cleft palate in the mouse.

Epidermal growth factor (EGF) injected into pregnant mice increased the frequency of cleft palate (CP) in cortisone-treated mouse fetuses. EGF alone produced proliferation and thickening of the epithelium of the palatal processes, but CP was not significantly increased over saline injected controls. Cortisone alone produced thinning of the palatal epithelium and caused CP in 61 percent of formed fetuses. The combination of EGF and cortisone treatment induced CP in 100 percent of formed fetuses; epithelial thickening still occurred with the combination treatment. Thus, EGF may be teratogenic under special circumstances. These observations suggest that the relative thickness of the palatal shelf epithelium may not be a critical factor in the fusion of the palatal shelves.

Abnormalities, Drug-Induced↗

PubMedDate not supplied

Gastrointestinal processing of gastrically administered prostaglandin F2 alpha in suckling and weanling rats.

In view of the presence of prostaglandins in milk, studies were performed to determine if maturational differences are present in the gastrointestinal processing of exogenous prostaglandin F2 alpha. Suckling (10- to 14-day-old) and weanling (30-day-old) rats were gastrically fed 3H-labeled prostaglandin F2 alpha and killed 2 h later. Characterization of radioactivity present in the stomach from animals of both age groups revealed low prostaglandin F2 alpha metabolism. Analyses of proximal, middle, and distal intestinal segments revealed age-related differences in prostaglandin metabolism. The amount of unmetabolized PGF2 alpha in intestinal segments of suckling rats ranged from 11.4 to 13.5%. In weanling rats, there was a tendency toward increased amounts of intact PGF2 alpha in proximal (16.3 +/- 1.6%) and middle (17.6 +/- 2.2%) regions; however, 25.9 +/- 1.9% of the tissue radioactivity present in the distal small intestine of weanling rats was authentic PGF2 alpha, significantly greater than that of the distal segment of suckling rats. The intestine of weanling rats contained greater amounts of less polar metabolites. These results indicate that orogastrically fed prostaglandin can pass through the gastrointestinal tract of developing rats and be delivered to the distal intestinal mucosa intact.

Animals↗

PubMedDate not supplied

In vitro intestinal processing of prostaglandin F2 alpha in rats: developmental aspects.

Cytoprotective prostaglandins (PGs) such as PGF2 alpha are present in milk and can be absorbed intact from the gastrointestinal tract. Developmental differences in gastrointestinal PG metabolism in vivo have been previously reported. To evaluate the role of small intestinal tissue, detailed analysis of PG processing in vitro of small intestinal segments of suckling and weanling rats using everted sacs was performed. Sacs were incubated in Krebs-Ringer bicarbonate buffer, pH 7.4, at 37 degrees C. Bathing mucosal fluid contained [3H]PGF2 alpha. Proximal and middle intestinal segment everted sacs of suckling rats exhibited greater transfer of radioactivity in comparison with those of weanling rats. Whereas in suckling rats transfer of radioactivity decreased from proximal to distal regions of the intestine, no segmental differences were seen in weanlings. Maturational differences were also present in the capacity for PG catabolism. Suckling rat everted sacs had a greater proportion of radioactivity present as intact, unmetabolized PGF2 alpha present in sac tissue and serosal fluid in all intestinal segments in comparison with those of weanling rats. Weanling everted sacs contained greater amounts of less polar metabolites. These results indicate that there are quantitative and qualitative age-related differences in transfer and processing of exogenous PGF2 alpha in small intestinal tissue of developing rats.

Animals↗