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Biomedical subjects

A Cuschieri

Publications and source records attributed to A Cuschieri.

At least 181 records · Page 10Linked to original sources

Wound infection rates: the importance of definition and post-discharge wound surveillance.

The importance of definition and post-discharge wound surveillance on reported wound infection rates have been studied, using data taken from a prospective, randomized, placebo-controlled, double-blind trial of the effect of whole body disinfection on postoperative wound infection rates. All patients admitted for an elective clean or potentially contaminated surgical procedure over a 32-month period were recruited into the study. Of the 3733 patients recruited, 3466 completed the study. Wound infection (which is defined for this study) is the main outcome that was examined. The effect of careful post-discharge follow-up of patients to look for wound infections was analysed for age, wound type and presence or absence of a drain. Sixty percent of postoperative wound infections occurred after discharge. A rising wound infection rate with increasing age was observed in the in-hospital cohort whereas the incidence of outpatient wound infections declined with age. This finding is attributed to the longer in-hospital stay encountered in patients above 50 years old (median, 3 vs. 5 days, P < 0.00001). Although clean operations had a significantly lower in-hospital infection rate, potentially contaminated procedures had a lower outpatient infection rate. A similar picture was observed in other subgroups of operations including horizontal versus vertical wounds and use of drains. When assessing reported wound infection rates, the definition of wound infection used and the extent of follow-up must be known to enable accurate assessment of the results.

Age Factors↗

The prognostic significance of the accumulation of p53 tumour-suppressor gene protein in gastric adenocarcinoma.

We have studied the expression of p53 in 206 patients with gastric adenocarcinomas. A standard immunohistochemical technique employing the CM-1 anti-p53 polyclonal antibody was applied to the routinely fixed and paraffin-embedded material from these tumours; overexpression of p53 was defined as positive nuclear staining: 46% (94/206) of gastric carcinomas expressed high levels of p53. There was no significant correlation between p53 positivity and the tumour grade, growth pattern, the Lauren type or lymph node metastases. Correlation with disease stage was only marginally significant (P = 0.05). Life table analysis revealed a highly significant association between p53 expression and survival (P = 0.0062), the odds ratio of death being 1.89 (95% confidence interval 1.33-2.69). The overall 5-year survival of patients with p53-positive tumours was 3% compared with 16% for those with p53-negative tumours (median survival time being 5.6 and 11.4 months respectively). These data suggest that overexpression of the p53 oncoprotein is an independent marker of shortened survival in gastric cancer patients.

Adenocarcinoma↗

Preface.

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Journal Article↗

Laparoscopic Management of Complicated Ulcer Disease.

Laparoscopic surgery for complicated ulcer disease has to be tailored to the specific needs imposed by the pathology and clinical state of the patient. In the elective situation, patients with concomitant reflux esophagitis and duodenal ulcer disease are best treated by partial crurally-fixed posterior fundoplication and highly selective vagotomy. Patients with resistant prepyloric ulcers and those on long-term medication with ulcerogenic drugs require truncal vagotomy and antrectomy. Because many patients with benign pyloric stenosis are elderly and exhibit hypochlorhydria, a drainage procedure without vagotomy is sufficient. The type of drainage performed, gastrojejunostomy or pyloroplasty, depends on the extent of scarring of antroduodenal segment. In the emergency situation, patients with perforated ulcer disease are best treated by simple closure with adequate peritoneal toilet. A definitive procedure should be reserved for fit patients with prior symptoms and limited chemical peritonitis of less than 6 to 8 hours duration. All perforated gastric ulcers should be biopsied at the time of laparoscopic closure. New endogastric techniques are being evaluated for the treatment of patients with bleeding gastroesophageal lesions.

Journal Article↗

Endoscopic Sympathectomy.

Denervation of the sympathetic nerve can be accomplished with much less operative trauma by using the endoscopic surgical approach. Although endoscopic thoracic sympathectomy is not new, the techniques and the extent of denervation have not been standardized. The anatomic appearances and configurations of the sympathetic chain are explained, and various procedures, including ramisections, interganglionic sympathectomy, and ganglionectomy, are reviewed. Thoracoscopic approaches, operative techniques, and potential complications are discussed.

Journal Article↗

Therapeutic laparoscopy.

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Cholangiopancreatography, Endoscopic Retrograde↗

A randomized trial of immediate discharge of surgical patients to general practice.

BACKGROUND: This study compares, in clinical and economic terms, out-patient follow-up with immediate discharge to general practice of patients undergoing any one of 29 defined surgical procedures. METHODS: A randomized controlled trial was undertaken in which patients recruited from two general surgery wards in Ninewells Hospital, Dundee, were randomized to follow-up care in the out-patient clinic or in general practice. Outcome was measured as clinical effectiveness in terms of morbidity and mortality; economic costs to the hospital compared with general practice; patient benefits and satisfaction; and General Practitioners' (GPs') opinions of the system. RESULTS: A total of 455 patients were randomized to outpatient and 454 to general practice follow-up. They were followed up for a minimum of six months. There were no differences in readmission rates, mean number of operations or mortality. The difference between the groups in the total health service costs was very small (2.68 pounds per patient more for those receiving out-patient follow-up). More of the general practice group preferred general practice care than the out-patient group preferred out-patient care (p = 0.03). The patient's travel costs and travel and treatment time were greater for the out-patient group (27.99 pounds, 113 min) than for the general practice group (24.90 pounds, 82 min). The GPs felt they had been given adequate information in the discharge documentation and were willing to accept immediate discharge as normal policy, although they expected it to increase their workload. If immediate discharge were instituted, the time saved in an out-patient clinic session of 40 patients would be an estimated 54 minutes, enough to see three extra new patients. CONCLUSION: General practice based follow-up care for this group of patients is as effective as, but less costly than outpatient care and is acceptable to GPs. Because of only small differences in costs between the two forms of follow-up, real gains to the health service will depend on the use of the time freed by a reduction in follow-up appointments in the out-patient clinic.

Aftercare↗

Diagnostic yield and management benefit of laparoscopy: a prospective audit.

A prospective audit of the diagnostic yield and management benefit of laparoscopy was undertaken in 220 consecutive patients. The procedure was performed electively in 180 patients and as an emergency in 40. The indications for laparoscopy in the elective group were suspected hepatic disease, staging of intra-abdominal malignancy, diagnostic problems, and chronic abdominal pain. Emergency laparoscopy was performed in patients admitted with acute abdominal pain. Diagnostic benefit varied with the indication for the procedure: liver disease 71%, tumour staging 87%, uncertain diagnosis 74%, acute abdominal pain 100%, and chronic abdominal pain 41%. Clinical management was significantly influenced by laparoscopy in 15 of 21 (71%) patients with liver disease, 10 of 30 (33%) with intra-abdominal malignancy, 5 of 19 (26%) with uncertain diagnosis, 32 of 40 (80%) with acute abdominal pain, and 15 of 110 (23%) patients with chronic abdominal pain. A wrong assessment of the nature or stage of the disease was made by laparoscopy in 3 of 220 (1.0%). There was no morbidity or mortality attributed to laparoscopy in the study.

Abdomen, Acute↗

Immunoscintigraphy of primary colorectal cancers with indium-111 monoclonal antibody B72.3.

Immunoscintigraphy with CYT-103, an 111indium-labelled immunoconjugate of B72.3, was evaluated in 10 patients before surgery for suspected or biopsy-proven primary colorectal cancer. The imaging results were compared with computed tomography (CT) findings at surgery, histopathology and immunohistochemistry. There were no adverse reactions following the administration of 1.0 mg 111In-CYT-103. Surgical and pathological findings identified 15 sites of disease (10 primary and five metastatic) and all but one lesion (severe dysplasia) were malignant. CT detected nine of 14 sites of malignancy compared to 12 as identified by immunoscintigraphy. It failed to detect two primary lesions and one case of peritoneal metastasis, all of which were imaged by CYT-103. Both imaging modalities failed to detect two of three cases with lymph node metastases and the dysplastic lesion (true negatives). The results indicate that 111In-CYT-103 imaging exhibits high sensitivity and specificity in the detection of primary and secondary lesions in patients with colorectal cancer.

Adenocarcinoma↗

Bilateral endoscopic splanchnicectomy through a posterior thoracoscopic approach.

The technique of bilateral total splanchnicectomy performed through a posterior thoracoscopic approach is described. The advantages of this route include excellent visual exposure of the neural anatomy of the sympathetic and avoidance of single lung anaesthesia. The procedure was performed for the relief of intractable pain in patients with advanced pancreatic cancer (n = 3) and patients suffering from chronic pancreatitis (n = 5). Persistent relief of pain until death was obtained in the patients with pancreatic cancer (2, 4, 6 months). In patients with chronic pancreatitis, the benefit to date has varied with the severity of the disease. In two patients with severe advanced disease and previous percutaneous blocks, the relief of pain lasted only 3 and 5 weeks and both patients required resection for renewed intractable pain. In three patients with minimal change disease, relief of pain has been good in the short term (maximum follow-up of 8 months). Bilateral thoracoscopic total splanchnicectomy merits further evaluation in patients with pancreatic pain. No complications including hypotension have been encountered.

Denervation↗

Laparoscopic surgery of the pancreas.

Diagnostic laparoscopy provides useful information in patients with pancreatic disease and is the most reliable technique for the staging of patients with pancreatic cancer. The advent of laparoscopic contact ultrasonography has enhanced the diagnostic and staging potential of laparoscopy. In addition to laparoscopic cholecystectomy for acute gallstone-associated pancreatitis, the following operations have been performed laparoscopically: bilio-enteric bypass and gastrojejunostomy in patients with advanced pancreatic cancer; internal drainage for pseudocysts; resection of insulinomas; distal resection for chronic pancreatitis; and pancreaticoduodenectomy for pancreatic cancer. Aside from cholecystectomy, there is as yet, insufficient information to conclude on the advantages of these laparoscopic approaches although the early results, particularly in the palliation of patients with malignant jaundice, are promising. Bilateral thoracoscopic splanchnicectomy for the relief of intractable pancreatic pain is also under current evaluation.

Biopsy↗

Thoracoscopic subtotal oesophagectomy.

A technique for endoscopic-assisted oesophagectomy is described using either a lateral or prone position for the oesophageal dissection. In a consecutive series of 34 patients, two were found to have hepatic deposits at preliminary laparoscopy, and four were inoperable at thoracoscopic staging. A further two had fibrous obliteration of the pleural cavity. Of the 26 procedures, 20 were performed in the lateral position and six in the prone position. There was one conversion to open thoracotomy due to massive aortic bleeding. There were no deaths. Postoperative complications included pneumonia (n = 3), recurrent laryngeal nerve palsy (n = 2) and one anastomotic leak. The median postoperative stay was 12 days (range 9-30 days). It is suggested that the prone position has technical advantages and reduces the postoperative respiratory complications.

Esophageal Neoplasms↗

Analysis of surgical movements during suturing in laparoscopy.

Laparoscopic suturing is now recognized as a necessary component of minimally invasive surgery. Until recently it has been avoided due to the lack of proper instrumentation and systematic technique. The principles of magnified surgery are discussed as they apply to the laparoscopic field, with emphasis on visual perception, economy of motion, choreography of movements, and "flawless technique". The principles and techniques of needle loading, handling, and driving are presented, as are the series of movements involved in tying an intracorporeal square-slip knot.

Equipment Design↗

Factors affecting quality of informed consent.

OBJECTIVE: To examine the factors influencing quality of informed consent. DESIGN: Prospective study comprising interviews with patients and patients' completing standard questionnaires. SETTING: Academic surgical unit of large teaching hospital. PATIENTS: 265 patients undergoing intrathoracic, intraperitoneal, and vascular surgical procedures. Of these patients, 192 have been followed up for six months. MAIN OUTCOME MEASURES: Patients' recall of information at various points in the study; this score was compared by age, provision of written information, cognitive function, intelligence quotient (IQ), mood state and personality traits, and health locus of control. RESULTS: The patients were best informed immediately after signing the consent form and from then on recall of information deteriorated. A total of 172/250 (69%) patients admitted to not reading the consent form before signing it. Old age adversely affected recall of information at all assessment points. Impaired cognitive function reduced information recall only during the stay in hospital. Patients with above average IQs handled information better than those with a lower IQ except immediately after the signing of consent forms. Patients with an internal locus of health control (that is, those who believed their health to be in their own control) were better informed than those with an external locus of health control. Operation information cards improved recall only on the day of discharge. CONCLUSION: Elderly patients and patients with below average IQ, impaired cognitive functions, and an external locus of control have poor information recall. Written information may be more useful if given before admission to hospital.

Adult↗

Cost-effectiveness of endoscopic surgery.

Endoscopic surgery is a rapidly defusing treatment approach subject to continual technological advance. It offers great potential for economic benefits as well as health gains, but as shown in the article in this issue by Kesteloot and Penninckx, the relative cost of open versus endoscopic surgery is a quite complex issue. The main factors influencing the costs and effectiveness of endoscopic surgery are reviewed and the need for more and better evaluation studies highlighted.

Cholecystectomy, Laparoscopic↗

Expression of p53 protein in normal, dysplastic, and malignant gastric mucosa: an immunohistochemical study.

Mutations in the p53 nuclear oncogene are the most frequent genetic abnormalities encountered in human malignancies. Using the polyclonal antibody CM-1, we have examined the expression of the p53 oncoprotein immunohistochemically in archival material of normal, dysplastic, and malignant gastric mucosa. Abnormal expression of this protein was not observed in biopsies of normal gastric tissue (n = 30) but was detected in 22 of the 36 gastric cancers analysed (61 per cent). Nuclear staining was diffuse in 15 of the positive cancer cases, the remaining seven showing a more varied heterogeneous staining pattern. Abnormal p53 protein was not detected in mild (n = 14) or moderate (n = 16) gastric dysplasia but was present in 3 out of 15 severe dysplasia cases. The results suggest that expression of the p53 oncoprotein is a common finding in gastric cancer and occurs as a late event in the malignant transformation process.

Antibodies↗

Monoclonal antibodies to cytoskeletal proteins: an immunohistochemical investigation of human colon cancer.

Monoclonal antibodies raised to a number of microfilament-associated proteins were shown to recognize the appropriate proteins in extracts from human colon tissue. They were then used in an immunohistochemical study of normal colonic mucosa, adenomas, and adenocarcinomas. A strong reaction was seen in stromal cells within the tumours (both adenomas and adenocarcinomas) when frozen sections were stained with antibodies to filamin and caldesmon. In addition, a similar reaction was seen in the adenocarcinomas when stained with antibodies to talin and gelsolin. We believe that immunohistochemical staining with these antibodies reveals a tumour-induced process in the surrounding cells, possibly related to a host response to tumours.

Adenocarcinoma↗

Localization of insulinoma by laparoscopic infragastric inspection of the pancreas and contact ultrasonography.

A new technique for localizing pancreatic insulinoma is described. This consists of a laparoscopic examination of the body and tail of the pancreas through an infragastric approach combined with contact ultrasonography of the gland. Accurate localization of an occult insulinoma in the body of the pancreas was achieved in one patient with the technique described. Laparoscopic contact ultrasonography of the pancreas by the infragastric approach should be as reliable as open intraoperative ultrasonography and constitutes the first step in the laparoscopic treatment of insulinoma.

Female↗