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Biomedical subjects

A Cooke

Publications and source records attributed to A Cooke.

At least 217 records · Page 12Linked to original sources
PubMed1979

Rabbit anti-EL4 serum. A reagent which discriminates between murine cytotoxic and suppressor cells.

Antisera against the C57B1 (H-2b) mouse lymphoma, EL4, were prepared in rabbits. After absorption with mouse liver, red cells and thymocytes, the antisera appeared to be cytotoxic for a subpopulation of peripheral T cells. The absorbed antisera blocked the immunosuppressive function observed when spleen cells from mice undergoing a graft versus host reaction were added to cells responding in vitro to sheep erythrocytes. This antiserum was unreactive against the cytotoxic cells also induced in this system. These results substantiate our previous finding that these antisera are specific for T-suppressor as opposed to T-helper or cytotoxic lymphocyte subpopulations.

Animals↗
PubMed1978

Activity of serum alpha-amylases in cystic fibrosis.

A new method which uses a differential inhibitor to measure pancreatic and salivary type alpha-amylases (EC 3.2.1.1) was applied to serum samples from 46 cystic fibrosis (CF) patients (age range 4-14 years) and 50 controls of the same age group. The levels of pancreatic type amylase were lower in the CF patients (median 26.5 I.U./1) than the controls (median 81.5) (P less than 0.001). The results for salivary-type enzyme, however, did not support the previously reported finding of higher than usual levels in CF patients. This discrepancy is probably due to differences in analytical methods. It is felt that this procedure will be of value in the investigation of patients for cystic fibrosis and other pancreatic disorders.

Adolescent↗
PubMed1978

Dose response, wavelength dependence and rate of excision of ultraviolet radiation-induced pyrimidine dimers in mouse skin DNA.

The yield of thymine-containing dimers produced in mouse skin DNA in vivo by 290 nm ultraviolet radiation was shown to increase with dose up to around 2000 J/m2 and subsequently at a much slower rate up to 8000 J/m2. The study of wavelength dependence of dimer formation in skin indicated that 290 nm was the most effective wavelength of those investigated, followed by 300, 280 and 260 nm, with 310 nm being by far the least effective. A reduction in the number of dimers present in skin DNA was shown to occur by 24 h post-irradiation in a dose-dependent manner. A significant percentage of the dimers was, however, found to persist in the skin until at least 72 h post-irradiation.

Animals↗
PubMed1977

Hyper-responsiveness in NZB mice to the experimental induction of anti-red cell autoantibody.

Strain differences in ease of induction of autoantibody production were observed when mice were injected with rat RBC. Responsiveness was not linked to the H-2 locus. NZB and (NZB X BALB/c)F1 mice were hyper-responsive both in terms of the induction of autoantibody and in the production of agglutinating antibody to rat RBC. C57BL/c and (C57BL X BALB/c)F1 were poor responders. Injection of the rat RBC in FCA converted a poor responder into a good responder. Adult thymectomy and ALS treatment did not significantly enhance autoantibody production.

Animals↗
PubMed1977

Characterization of haemagglutinating factor produced by mouse spleen and lymph node in culture.

Mouse spleen cells produce haemagglutinating activity in culture which is species specific but not strain specific. The activity is produced by all strains of mice tested including nude mice. It is produced in cultures of spleen and lymph node but not of peritoneal exudate cells. Production of the factor is not affected by macrophage depletion of the spleen. This activity is not antibody mediated.

Animals↗