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Biomedical subjects

A Contestabile

Publications and source records attributed to A Contestabile.

120 records · Page 7Linked to original sources
PubMed1976

Histochemical characterization of cholinesterase activity in the frog brain with special reference to its localization on the wall of blood vessels.

The nature of the cholinesterase activity present on the wall of blood vessels in amphibian brain has been studied histochemically. It is concluded that the enzyme involved is acetylcholinesterase rather than butyrylcholinesterase, which more frequently occurs in the blood vessels of the brain of other vertebrates. The histochemical results are in agreement with most biochemical data concerning substrate specificity and inhibitor response for both acetylcholinesterase and butyrylcholinesterase. The two main hypotheses put forward by others in order to explain cholinesterase activity in brain vessels are discussed briefly.

Acetylcholinesterase↗
PubMed1976

The fine localization of ATPases in cultures in vitro of chick embryo spinal cord.

A histochemical study of the ultrastructural localization of ATPases in cultures of chick embryo spinal cord has been carried out. The localization of Ca2+ and Mg2+ activated membrane ATPases appears similar: both enzyme activities are localized on the outer surfaces of plasma membranes of all kinds of cell present in the cultures, with the exception of the membranes in direct contact with the culture medium. The results are discussed in relation to data concerning the localization and function of ATPases in vivo and in relation to the possible establishment of mechanisms of nutrient uptake and transfer in cultures of nervous tissue.

Adenosine Triphosphatases↗
PubMed1975

Histochemical localization of acetylcholinesterase in the cerebellum and optic tectum of four freshwater teleosts.

A histochemical study has been carried out on the localization of acetylcholinesterase (AChE) in the cerebellum and optic tectum of four species of freshwater teleosts. AChE distribution in the cerebellar cortex of teleosts shows differences among the species examined and, in the trout, also differences between different cerebellar areas. This uneven kind of enzyme distribution corresponds to a similar variety of AChE patterns noticed in other vertebrates, especially mammals. AChE distribution in the optic tectum shows a prevalent pattern characterized by precise laminar distribution of enzymatic activity which is alternatively strong, weak or absent in the different tectal layers. The results suggest that most of sensitive imput and many systems of stimuli propagation may be mediated by cholinergic mechanisms in the optic tectum of telecosts.

Acetylcholinesterase↗
PubMed1975

Histochemical study on the distribution of some enzyme activities in the vagal and facial lobes of the goldfish, Carassius auratus.

The histochemical localization of six enzymic activities (acetylcholinesterase, pseudocholinesterase, monoamine oxidase, lactate dehydrogenase, succinate dehydrogenase and glucose-6-phosphate dehydrogenase) has been studied in the vagal and facial lobes of the goldfish, Carassius auratus. These encephalic centers are hypertrophic in Cyprinidae, corresponding to the dominance of gustatory function. Acetylcholinesterase shows a complex laminar distribution in the vagal lobes and a peculiar cellular localization in vagal motor neurons. Monoamine oxidase activity is mainly evident in fibrous tracts coming to or leaving from the lobes. Among oxidative enzymes examined, lactate dehydrogenase and succinate dehydrogenase exhibit distribution patterns respectively similar to those observed for acetylcholinesterase and monoamine oxidase. Some features on enzymes distribution in the gustatory centers of Carassius are in agreement with the enzymatic patterns well known in higher vertebrates.

Acetylcholinesterase↗
PubMedDate not supplied

Choline acetyltransferase activity in the cerebellum and in centers of lateral line system of teleosts.

Using choline acetyltransferase as a marker of cholinergic activity, different cerebellar areas and brainstem nuclei were assayed in the goldfish and the catfish. Enzyme activity resulted remarkedly higher in archicerebellum than in paleocerebellum. In addition three brainstem nuclei related to lateral line system, showed high or very high levels of choline acetyltransferase. The results suggest that cholinergic transmission would play an important role in central circuits of lateral line system, including the projection towards archicerebellar areas.

Animals↗