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Biomedical subjects

A Cipriani

Publications and source records attributed to A Cipriani.

At least 91 records · Page 5Linked to original sources
PubMed1985

Impaired production of interleukin-2 in peripheral blood of patients with sarcoidosis.

In twelve patients with active sarcoidosis we attempted to explain the nature of reduced release on interleukin-2 (IL-2) and the consequent impairment in lymphoproliferative in vitro responses. The addition of exogenous IL-2 containing supernatants was unable to completely restore the defective uptake of 3H-Thymidine suggesting that an impairment of IL-2 producer cells is not enough to explain the in vitro hyporesponsiveness of sarcoid lymphocytes. We also found a reduced number of precursors of IL-2 responder cells, as defined by peripheral blood lymphocytes bearing Tac determinant following in vitro stimulation with mitogens. The abnormalities of both IL-2 producer cells and precursors of IL-2 responder cells in peripheral blood of patients with sarcoidosis are discussed, stressing the importance of the concept of compartmentalization of T lymphocytes in this disease.

Adult↗
PubMed1984

Immunohistological study in sarcoidosis: evaluation at different sites of disease activity.

Sarcoidosis is a multisystem disease characterized by enhanced immune responses at sites of involvement. For this reason, an immunohistological study using monoclonal antibodies against T-cell subpopulations was carried out in order to evaluate the topographic distribution of immunocompetent cells in tissue sections obtained from a variety of involved organs, such as parenchymal lung, lymph nodes, eyes, skin, and liver. Biopsy specimens were also stained for detection of immunoglobulins, complement, and fibrinogen deposits. The data demonstrate a redistribution of T cells from the blood to all the sites of disease activity where they account for the large majority of infiltrating cells, both in the early lesions (merely a lymphocytic infiltrate) and in well-organized granulomas. Moreover, these cells express a helper-related phenotype, as demonstrated by the high Leu-3/Leu-2 ratios, at sites of involvement with respect to the blood (blood, 1.8/1; transbronchial lung biopsies, 10.5/1; lymph nodes, 19/1; skin, 28/1; liver, 22/1; eye, 14/1). In line with this helper infiltration is the presence of plasma cells and immunoglobulin deposits, suggesting a local hyperreactivity of the B-cell immune system. Both the hypergammaglobulinemia and the T lymphopenia usually observed in the blood of sarcoid patients could be explained by these observations. Comparative analysis of immunohistological data and bronchoalveolar lavage (BAL) findings provides further evidence that BAL cellularity reflects the changes already occurring in lung histology. The studies emphasize the importance that immunological phenomena play in the pathogenesis of sarcoidosis and provide new insights into the mechanisms leading to the formation and maintenance of the sarcoid granuloma.

Adult↗
PubMed1984

Evidence of cells bearing interleukin-2 receptor at sites of disease activity in sarcoid patients.

The frequency of cells reactive with anti-Tac monoclonal antibody (MoAb), which recognizes the interleukin-2 (IL-2) receptor, has been evaluated in cell suspensions from peripheral blood and bronchoalveolar lavage (BAL), and in frozen sections from involved tissues in 18 patients with active sarcoidosis. Peripheral blood lymphocytes of sarcoid patients do not bear Tac determinant and reduced numbers of Tac+ cells are inducible following PHA stimulation. On the other hand, significant numbers of lymphocytes reactive with anti-TacMoAb are present in the cells obtained from the BAL and a number of Tac+ cells infiltrate the lung, lymph node and conjunctiva. The finding of Tac+ cells in the BAL fluid and in other organs in patients with sarcoidosis provides evidence that some T cells in these involved tissues have the characteristics of IL-2 responder cells and thus the potential to absorb IL-2, supporting the hypothesis that T lymphocytes replicate in situ at sites of disease activity.

Adult↗
PubMed1984

Distribution of natural killer cells in sarcoidosis.

A number of immunological abnormalities have been reported in sarcoidosis. In this paper an excess of Natural killer (NK) cells, as defined by the reactivity with HNK-1 monoclonal antibody, is demonstrated in peripheral blood of these patients. On the contrary, only a few HNK-1+ cells have been found among mononuclear cells infiltrating and/or surrounding sarcoid granulomas in the lungs, lymph nodes and skin. While NK cell activity may represent one of the first lines of natural resistance against foreign antigens, the lack of killing in involved tissue gives no support for control of sarcoidosis by NK cells at sites of disease activity. Possible interpretations of these findings are discussed.

Adult↗
PubMed1982

[Circulating immune complexes in a population of subjects seropositive for rheumatoid factor].

The level of immune complexes has been detected with the C1q binding test in 179 subjects positive by Latex agglutination test (titre greater than 1/20). The average of C1q binding was 5.36%, significantly higher than in 30 normal blood donors. A positive correlation was observed between the percent of C1q binding and, respectively, the titre of the latex test and the erythrocyte sedimentation rate. Of the 36 patients with rheumatoid arthritis, 16 (45.7%) showed an high level of C1q binding activity (greater than 5%), as well as 4 of the 8 patients with chronic hepatitis (50%) and 11 of the 31 patients with infectious diseases. No immune complexes were detected among the 11 healthy latex positive subjects.

Antigen-Antibody Complex↗
PubMed1981

[Typical, atypical and malignant bronchial carcinoid].

A series of 54 bronchial carcinoids operated in the course of 12 yr is presented. There were three histological types: typical, atypical and malignant. A parallel was also apparent between histological appearance and clinical pattern. Correct prognosis, however, demands the examination of other parameters, such the tendency of the neoplasia to infiltrate, and the presence of metastatic lymph nodes.

Adolescent↗
PubMed1980

Imbalance in T gamma and T mu lymphocyte subpopulations in patients with sarcoidosis.

The percentage and the absolute numbers of T lymphocytes Fc receptors for IgG and IgM (T gamma and T mu lymphocytes, respectively) were evaluated in fourteen patients with active sarcoidosis and in a group of controls. A marked increase in the percentage of T gamma cells and a net decrease of T mu cells were found. Although sarcoidosis patients present a T lymphopenia, the absolute number of T gamma lymphocytes was still increased whereas the T mu decrease was even more pronounced. The possible causes of this imbalance in view of the recent interpretations of T suppressor and T helper lymphocytes are discussed.

Adult↗
PubMed1977

[Cellular immunity in sarcoidosis].

The characteristics of the circulating lymphocytes of 24 patients suffering from sarcoidosis were studied. The surface markers and the in vitro lymphocytic activity, both spontaneous and after stimulation with PHA, were assessed. Each culture was carried out in both homologous and autologous plasma. The stimulating ability of the plasma of sarcoidosis patients on normal lymphocytes was also evaluated. The patients were divided into two groups, i.e. acute sarcoidosis and sarcoidosis in remission. From this study it can be stated that depression of T-dependent immunity is particularly evident in patients with active disease. This depression seems to be mainly due to the presence in the plasms of factors which inhibit the cellular immune response.

Adult↗
PubMedDate not supplied

Sleeve lobectomy in the treatment of bronchogenic carcinoma.

From 1980 to 1985, 44 sleeve lobectomies were carried out in patients with bronchial cancer. Sixteen patients received preoperative radiotherapy. Perioperative mortality was 6.8%. There were seven anastomotic complications (three fistulae and four stenoses) and two recurrences at the anastomosis. Overall actuarial survival was 45% at four years. These results seem to suggest that sleeve lobectomy should be considered an elective rather than a compromise procedure and a viable alternative to pneumonectomy. Preoperative radiotherapy neither increases complications nor has a negative effect on outcome. It contributes towards reducing local recurrences and maximizes tissue salvage. Long-term survival is related to stage or histology, factors generally governing the survival of lung cancer operated patients, although the TNM classification is ill-suited to identifying tumors which can be resected by a sleeve lobectomy.

Bronchi↗