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A Cattaneo

Publications and source records attributed to A Cattaneo.

210 records · Page 12Linked to original sources
PubMedDate not supplied

Enzymes of human cervical mucus: a comparative study on fertile and infertile mucus and on the endocervical epithelium.

We report a comparative study on protein and enzyme content of human periovulatory cervical mucus and endocervical epithelium. The salient results of our investigation are summarized in the following three points: 1) The method of taking of cervical mucus samples may markedly affect the results of protein and enzyme assays. It is warmly advisable to collect mucus samples from the endocervical canal. 2) Cervical mucus from women diagnosed as having cervical-factor infertility is oligoproteic and lacks some enzyme activities always present in normal cases. 3) Human cervical mucus and endocervical epithelium are very similar as for the enzyme distribution, but show quite different LDH-isoenzyme patterns. In the latter tissue, the H subunits constitute about 77 per cent of the whole LDH, suggesting that this enzyme here is functioning to produce pyruvate rather than lactate.

Adult↗
PubMedDate not supplied

Changes in enzyme levels in human cervical mucus during the menstrual cycle.

Cervical mucus is a complex secretion produced by the endocervical epithelium. A comparative analysis of 30 enzymes was carried out on 15 women during their periovulatory period (days 10 to 16 of the menstrual cycle). Nine different enzymes were always found to be present in the cervical mucus. Changes in enzymatic activity were further studied in two of the women, one with a normal (27-day) and the other with a short (20-day) menstrual cycle. The data were compared with those for the same enzymes in the saliva of these two women. Each woman's ovulation time was determined through hormone measurements in urine and blood, as well as by recording basal body temperature and by Billing's method. Our results indicate that a maximum peak occurs for several enzymes during the periovulatory period.

Adult↗
PubMedDate not supplied

Some molecular features of human cervical mucus.

1) The time preservation effect of the enzymatic activities present in human cervical mucus has been studied. 2) In order to protect the enzymatic activities of the mucus from bacterial impurities, the enzymes studied were assayed in presence of NaN3. 3) The effect of an acid pH likes present in the vagina was also studied: a low pH exerts a marked inhibition on mucus enzymes. 4) the use of nonionic detergents for mucus solubilization does not produce a marked increase of the enzymatic activities studied. 5) A NADP-dependent malate dehydrogenase in peri- and post-ovulatory period has been identified. 6) The presence in mucus of at least four lactate dehydrogenase isoenzymes has been demonstrated and their absolute values determined.

Cervix Mucus↗
PubMedDate not supplied

[Calcium nephrolithiasis and phosphate therapy. Long term study (65 cases) (author's transl)].

Protected phosphate therapy was used in 65 cases of recurrent calcium nephrolithiasis. Mean duration treatment was 2 years and 1 month (more than 3 years in 17 cases). Mean lithiasis episodes by year-patients were 1,55 renal colics and 0.34 stone formation before phosphate treatment, versus 0.66 renal colics and 0.10 stone formation (more than 60% reduction), during treatment. There was simultaneously decrease of hypercalciuria (24 cases out of 42), of asthenia (16 cases out of 19), of signs of spasmophilia (12 cases out of 18) and disappearing of bone pains (4 cases out of 6). Side effects were rare. Minor digestive troubles were observed in 11 cases: diarrhea (3 cases) or gastralgias (9 cases). These side-effects necessitated discontinuation of thiazide therapy in only two cases and reduction of doses in 6 other cases. From our data, phosphate therapy appears an efficient drug in recurrent calcium nephrolithiasis. It acts in reducing levels of calciuria and enhancing urinary pyrophosphates excretion, inhibitors of calcium crystallization.

Adult↗
PubMedDate not supplied

[Extra-mammary Paget's disease. An HPV-correlated neoplasia?].

Eight cases of Paget's disease of the vulva and anogenital region are presented. Seven cases presented intraepithelial lesions and only one showed superficial intraepidermal diffusion. The average age of the patients in our series was 58.8 years. In all cases histochemical and immunohistochemical reactions were positive, except for the CEA reaction (only one case was positive). Specimens from our series were tested for presence of HPV 6, 11, 16, 18, 31, 33, 35 by in situ DNA hybridization. All cases were negative. Paget's disease, at the moment, is the only tumor of the low female genital tract non HPV-associated. Recurrences are very frequent even many years after radical surgery treatment.

Aged↗
PubMedDate not supplied

Selection of intracellular antibodies.

The intracellular expression of antibodies in mammalian cells is a strategy to inhibit in vivo the function of selected molecules. One limitation of this technology is represented by the unpredictable behaviour of antibodies, under conditions of intracellular expression. Recent advances in the field of antibody expression in E. coli show that the introduction of mutations inspired by sequence comparisons or by educated guesses can improve the properties of some antibody domains, but the general applicability of this approach to intracellular antibodies remains to be proven. As a complement to rational approaches, selection schemes whereby antibodies are selected on the basis of their performance in vivo as intracellular antibodies can be exploited. (Fig. 3, Ref. 44.)

Antibodies↗