Biomedical subjects

A Carracedo

Publications and source records attributed to A Carracedo.

148 records · Page 9Linked to original sources

PubMedDate not supplied

HRAS1 minisatellite alleles in colorectal carcinoma: relationship to microsatelite instability.

OBJECTIVES: To further evaluate sporadic colon carcinoma risk associated with rare HRAS1 VNTR alleles, the relationship with microsatellite instability and with HRAS1 VNTR instability. MATERIALS AND METHODS: The HRAS1 VNTR was genotyped in 121 tumors and normal samples from sporadic colon carcinoma patients (47 right and 74 left colon) and in 109 samples from healthy individuals. The HRAS1 alleles were identified using PCR and automatic fluorescent electrophoresis detection combined with MVR-PCR (Minisatellite Variant Repeat-Polymerase Chain Reaction). Microsatellite Instability (MI) was analysed with 10 microsatellite markers. RESULTS: A relative risk of 3.04 (95% CI: 1.16-4.92) associated with rare alleles was obtained. No HRAS1 minisatellite instability was present in the tumors. Samples with MI were equally distributed between the common and rare HRAS1 allele groups, while the distribution of HRAS1 alleles in samples with MI was similar in right and left colorectal carcinoma. CONCLUSION: Rare HRAS1 VNTR alleles are associated with colorectal carcinoma risk independent of the tumor location. MI is not likely to be involved in the same underlying defect that generates rare HRAS1 alleles in colorectal carcinoma.

Adenocarcinoma↗

PubMedDate not supplied

[DNA databases for criminal investigation purposes: technical aspects and ethical-legal problems].

Advances in DNA technology and the discovery of DNA polymorphisms have opened up the possibility of creating data bases of individuals' DNA for criminal investigation purposes. In many European countries laws are being drawn up or have been passed to regulate such data bases. In Spain theres is a need for an urgent ethical and legal debate on ther appropriateness. The article is divided into two parts. In the first, the authors discuss the technical principles underlying these data bases. In the second, they set out their legal and ethical views on regulation in Spain.

Databases, Nucleic Acid↗

PubMedDate not supplied

[Breakdown of the blood-retino-vitreal barrier after photocoagulation].

The degree of breakdown of the blood-retino-vitreal barrier after Argon laser photocoagulation, and the possibility of preventing it by topical or oral administration of indomethacin, have been investigated in a study involving 58 eyes of 29 rabbits. Protein levels in the vitreous, as determined by densitometry, were four times higher in photocoagulated than in non-photocoagulated eyes (p less than 0.005). Neither topical nor oral administration of indomethacin was successful in preventing post-photocoagulation breakdown of the blood-retino-vitreal barrier at the dosages employed.

Administration, Oral↗

PubMedDate not supplied

[Autistic syndrome associated with refractory temporal epilepsy].

Autism is a psychopathological disorder of childhood characterized by severe lapses in behaviour and cognition and excessive presence of extravagant unsuitable behaviour that affects a child's development. It is associated with different conditions of the Central Nervous System, especially a high incidence of epilepsy. We report a case of autistic syndrome with refractory temporal epilepsy, describing its clinical onset, (type of seizures, focal anomalies on the EEG, evidence of relation between limbic disfunction and autism.

Autistic Disorder↗