Biomedical subjects
A Capron
Publications and source records attributed to A Capron.
[Contribution of immunology in the diagnosis and treatment of parasitic diseases].
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[The immunological diagnosis of hydatidosis. 139 cases (author's transl)].
Hydatidosis is not rare in France and often poses diagnostic problems to practitioners. In addition to classical clinical and radiological data, immunological methods provide at the present time reliable means of confirming the diagnosis and conducting post-therapeutic surveillance. After a brief epidemiological and clinical review, the authors review the different laboratory techniques available and report their experience involving 139 cases collected over a period of 2 years. Emphasis is placed upon the relatively high prevalence of the disease amongst immigrant workers, as oppossed to the rare cases seen in the native population, as well as the value of immunoelectrophoresis and conditioned haemagglutination reactions. New therapeutic possibilities offered by mebendazole and its derivatives are indicated.
Cytophilic binding of IgE to the macrophage. III. Involvement of cyclic GMP and calcium in macrophage activation by dimeric or aggregated rat myeloma IgE.
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Schistosoma mansoni: ultrastructural damages due to complement on schistosomula in vitro.
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Dipetalonema viteae: microfilariae production in various mouse strains and in nude mice.
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Identification and measurement of rat eosinophil phospholipase D. Its activity on schistosomula phospholipids.
A sensitive assay, using [14C]lecithin as a substrate, has been developed for the measurement of phospholipase activity in rat peritoneal polymorphonuclear leukocytes. Cell extracts were found to contain a phospholipase D activity and indirect evidence suggested that eosinophils are responsible for the cleavage of lecithin. Intact peritoneal cells were also able to hydrolyze exogenous [14C]lecithin in vitro. When [3H]choline-labeled schistosomula were used as targets in antibody-dependent cytotoxicity experiments, the radioactivity of lecithin decreased more rapidly in a complete cytotoxicity system than in controls, suggesting that hydrolysis of schistosomula phospholipids occurred during the killing process.
Detection of Schistosoma mansoni M antigen in circulating immune-complexes and in kidneys of infected hamsters.
Circulating M antigen (CMA) of Schistosoma mansoni was found in the trichloroacetic acid (TCA) soluble fraction of a polyethylene glycol precipitate of serum from infected hamsters. It was also found as a TCA-soluble component in an immunoglobulin-containing fraction eluted from infected hamster kidneys. It was not found in similarly treated control sera nor in the products of acid dissociation of circulating immune complexes (CIC) from infected hamster sera. CMA was not detected in the kidneys of normal hamsters. Precipitating anti-M antigen antibodies were present in one of 10 sera from infected hamsters, but not in the eluates of hamster kidneys. These results indicate that CMA is present in circulating immune complexes in infected hamsters. The presence of CMA in kidneys from the same hamsters suggests a possible role for circulating antigens in immune-complexed form in the aetiology of glomerulonephritis in S. mansoni infection.
Application of thin layer immunoassay (TIA) as a serodiagnostic tool in schistosomiasis. A preliminary report.
Thin layer immunoassay (TIA) is a recently developed simple serological technique which, in a preliminary study, has been applied to the serodiagnosis of schistosomiasis. 64 of a total of 69 sera from patients with schistosomiasis were positive in TIA when a Schistosoma mansoni worm antigen preparation was used as coating material. A trial of the diagnostic specificity of the assay was made by cross-testing sera from patients with different parasitic diseases, using TIA plates coated with extracts from the relevant parasites. All sera from patients with filariasis, fascioliasis and echinococcosis were positive in homologous TIA systems. In heterologous systems, cross-reactivity was noted for some sera in all groups except fascioliasis. The largest proportion of cross-reacting sera was registered in the echinococcosis group. It is suggested that, after further evaluation, the TIA technique might supplement or be used as an alternative to other serological tests already in use for the diagnosis of schistosomiasis. In favour of the TIA technique are its simplicity and its low cost.
Contribution of radioisotope techniques to the evaluation of immunity in human and experimental schistosomiasis.
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Immunoregulation of parasite infections.
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Intramembrane particle movements associated with binding of lectins on Schistosoma mansoni surface.
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[Direct lymphocytotoxicity and antibody-dependent cell-mediated cytotoxicity in thyroid diseases].
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Kinetics of classes and sub-classes of total immunoglobulins and specific antibodies to Schistosoma mansoni during murine infection.
During the course of Schistosoma mansoni murine infection there is a dramatic increase of some immunoglobulins and S. mansoni-specific antibodies. The most substantial response is initiated after 40 days of infection and results in a prolonged increase of total IgG1, IgM and IgA. The maximum increase is respectively 26, 14 and 3-fold the basic immunoglobulin level in control mice. Some anti-S. mansoni classes and sub-classes were studied by an original radio-immunoadsorbent test. Anti-S. mansoni IgG1 and IgM antibodies appear and increase at the same time as that of total IgG1 and IgM. Anti-S. mansoni IgA antibodies appear later (80th day) and correspond to a second peak of total IgA.
Physicochemical characteristics of Listeria specific antigen 2.
Listeria specific antigen 2 (Ag2) was purified to within 97% of homogeneity, with a high yield, using both gel filtration and polyacrylamide gel electrophoresis. Ag2 is a glycoprotein. Its isoelectric point is about 4.2. As determined by sodium dodecyl sulphate-polyacrylamid gel electrophoresis, its molecular weight in 16710 +/- 450. Ag2 may aggregate easily since it was previously found in gel filtration in a peak corresponding to a molecular weight of 160000. No enzyme activity has been found in Ag2.
In vitro study of immunological events in human and experimental schistosomiasis: relationships between cytotoxic antibodies and circulating Schistosoma antigens.
Complement-dependent cytotoxic antibodies were found in 54% of Schistosoma mansoni infected patients from Burundi and in 69 to 78% of Schistosoma mansoni ninfected Brazilian patients. The levels of cytotoxic Ab were not statistically different in sera from infected mothers and from their newborn children, suggesting a transfer through the placenta. A sandwich radioimmunoassay (SRIA) and the Radioimmunoprecipitaion-PEG assay (RIPEGA) technique were used in order to detect respectively total schistosome circulating soluble antigens (CSA) and schistosome antigen '4' in sera from infected patients. An inverse relationship was found between the presence of cytotoxic Ab and both total CSA and antigen '4'. The cytotoxic Ab and total CSA levels were followed in five Erythrocebus patas monkeys for 30 weeks after Schistosoma mansoni infection. As in human schistosomiasis the presence of cytotoxic Ab was found to be inversely correlated with the presence of total CAS. The blocking role of Schistosoma mansoni antigens in a complexed form was suggested by the inhibitory effect of the ultracentrifugation pellet of infected human serum on the cytotoxic activity. Moreover, the CSA absorption of infected monkey serum by passage through an anti-CSA immunosorbent significantly increased the cytotoxic activity. Possible mechanisms for the inhibitory role of circulating immune complexes on complement-dependent cytotoxic activity are discussed.
In vitro and in vivo inhibition of mast cell degranulation by a factor from Schistosoma mansoni.
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Circulating immune complexes and anti-IgG antibodies in mucocutaneous leishmaniasis.
Circulating immune complexes (CIC), anti-IgG, anti-DNA, and anti-collagen autoantibodies (Ab) were investigated in sera from patients with South American leishmaniasis. No significant levels of anti-DNA or anti-collagen autoantibodies were observed. Only a few patients with cutaneous leishmaniasis or with only one mucocutaneous lesion showed values for CIC and anti-IgG Ab higher than those in the control group. In contrast, both CIC and anti-IgG Ab were demonstrated in most patients with several lesions due to mucocutaneous leishmaniasis (MMC). Moreover, a close correlation was noticed between the detection of CIC and anti-IgG Ab in MMC patients. This relationship suggested that part of the detected CIC could have been formed by IgG-anti-IgG complexes. The involvement of these immunopathologically active substances in the clinical evolution of mucocutaneous leishmaniasis is discussed.