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Biomedical subjects

A Calzolari

Publications and source records attributed to A Calzolari.

At least 73 records · Page 4Linked to original sources
PubMed1994

HPV and p53 in urinary bladder carcinoma.

We report a study on human papilloma virus (HPV) and p53 protein in 18 cases of urothelial carcinoma grade 1, 2 and 3. The presence of HPV has been correlated to the p53 protein expression, as this virus, once integrated in the cell nuclei, seems to cause the alteration of some genes expression, involved in the cell-cycle regulation, like p53. One case of urothelial papillary carcinoma grade 2, infiltrating the lamina propria, resulted to be positive for HPV type 31/33/51 and for p53 protein. Our data suggest that HPV type 31/33/51 may have played a role in the pathogenesis of this neoplasia causing an alteration of p53 gene.

Aged↗
PubMed1994

Expression of retinoblastoma protein in human breast cancer: an immunohistochemical study.

The product of the human retinoblastoma gene (pRb) is a nuclear phosphoprotein that is thought to function as a tumor suppressor. Heterogeneous expression of the Rb gene product contributes to the genesis of a diverse group of human neoplasma such as breast, prostate, small cell lung, bladder carcinomas and leukemia. The clinical importance of Rb function demands a reproducible Rb immunohistochemical assay to distinguish Rb+ from Rb- tumor cells. We report an immunohistochemical study to detect Rb protein in a series of 50 invasive breast cancers. Our results support the hypothesis that the Rb gene functions as both a cell growth factor and a tumor suppressor.

Breast Neoplasms↗
PubMed1993

Identification of staphylococci from bovine milk in Argentina.

Two hundred and thirty three strains of staphylococci isolated from bovine milk from 17 dairies in Argentina were classified into 16 species. Most of the strains (78%) belonged to 4 species; S. aureus, S. haemolyticus, S. hominis, and S. warneri (38.5, 20.5, 9.4 and 9.0%, respectively). The remaining 12 species appeared with low frequencies. Twenty-one of the strains were isolated from mammary quarters with signs of clinical mastitis. The majority of the milk samples infected with S. aureus or S. haemolyticus showed very high somatic cell counts.

Animals↗
PubMed1993

Detection of Epstein-Barr viral genome in tumor cells of Warthin's tumor of parotid gland.

The presence of Epstein-Barr virus (EBV) DNA-sequences in specimens of Warthin's tumor in the parotid gland was studied. Using an in situ hybridization technique, EBV genome was detected in distinct cell types in routinely processed tissues. The authors' results indicated that EBV is frequently present in the cytoplasm (possibly because of a chronic, productive infection) of neoplastic cells of multiple/bilateral Warthin's tumor (MBWT) (86.7%), and only occasionally of solitary Warthin's tumor (SWT) (16.7%). In addition, EBV genome has often been documented in the cytoplasm of ductal cells (75% of MBWT and 33.3% of SWT), and occasionally of acinar cells (16.7% of both MBWT and SWT) of residual normal salivary gland tissue surrounding both MBWT and SWT. The almost constant presence of EBV DNA in tumor cells of MBWT, as opposed to the occasional finding in SWT, suggested a strong association between infection of cells with this virus and the development of multiple/bilateral lesions. The positivity of ductal epithelium supported speculations about the role of ductal cells as a histogenetic precursor of the tumor. This was the first report on the detection of EBV genome in Warthin's tumor.

Adenolymphoma↗
PubMed1993

[Induction of bacterial translocation in rats with minimal doses of endotoxin].

OBJECTIVE: To determine whether a non lethal dose of intramuscular endotoxin in rats causes a physical disruption of the mucosal barrier and promotes the translocation of bacteria from gut to visceral organs. DESIGN: Prospective randomized trial. SETTING: Experimental laboratory university medical center. EXPERIMENTS: 60 male wistar rats, weighing 250 g. INTERVENTIONS: six hours and twenty-four hours after endotoxin (8 mg/kg) or saline (control group) given intramuscularly, rats were sacrificed and mesenteric lymph nodes, spleen and some segments of the ileum were removed. Lymph nodes and spleen were cultured for translocating bacteria while ileum segments were observed by light microscopy. RESULTS: Incidence of bacterial translocation increases from 6 hours (40% p < 0.05) to 24 hours (60%, p < 0.01) after endotoxin administration. Histologic evaluation of the ileum demonstrated that a mucosal injury occurred only at the 24 an hour and that it was characterized by an evident pronounced interstitial edema with focal breaks in the basal membranes of villous and glands. CONCLUSIONS: It appears that microorganisms and microbial products contained in the gut can cross the mucosal barrier and spread into other visceral organs also after a low dose of intramuscular endotoxin.

Animals↗
PubMed1992

Early identification of patients at risk for sinus node dysfunction after Mustard operation.

We studied 60 patients who had survived the Mustard procedure for transposition of the great arteries, performed between the ages of 2 days and 24 months (mean 4.51 +/- 3.79). All patients were given a postoperative 24-hour dynamic electrocardiogram at 15 days, 1 year and 3 years and then every 2 years. Those who were found to have sinus node dysfunction during follow-up, were given a dynamic electrocardiogram every 3-6 months. The average follow-up period was 38.7 +/- 19.8 months, median 36. Sinus node dysfunction was detected during follow-up in 20 patients (33.3% of the total), 8 of whom had had a pacemaker inserted. Risk factors for late development of sinus node dysfunction were found to include prolonged cross-clamping of the aorta during surgery (P = 0.003), especially over 50 minutes (relative risk 3.5:1), and the presence of even transient sinus node dysfunction after surgery (P = 0.006). These observations suggest, first, that sinus node dysfunction may develop after the Mustard operation as a combined effect of extensive atrial surgery and a long period of myocardial ischemia and, second, that the presence of the disease immediately after the operation sometimes indicates that it will recur or persist during follow-up.

Arrhythmias, Cardiac↗
PubMed1991

[The life style and physical activity of the child operated on for congenital cardiopathy].

The parents of 151 children operated on for congenital heart disease have answered some questions about the scholastic, extrascholastic and physical activity of their children. Approximately 94% of the children are at the correct school level for their age. Extrascholastic activities of the city children is the same as children living in the country. During their free time 22% of the child population engages in physical activity, whereas 78% of the child population engages in physical activity at school. Among the parents, 61% think the activity of their children is normal, and 27% think it is too active. Our results demonstrate that the children operated on for congenital heart disease have a normal life during scholastic and free time, whereas, the introduction of these children to sport activities is anomalous and insufficient.

Adolescent↗
PubMed1990

Rehabilitation of children after total correction of tetralogy of Fallot.

Nine children who had undergone total correction of tetralogy of Fallot were studied with the purpose of observing the physical changes that might be produced by a period of rehabilitation in hospital and comparing these results with those of a control group having the same pathology but which had not followed a protocol for physical activity. Precise criteria were used in the selection of the patients. Careful clinical examinations and exercise tests were conducted before and after the programme, in particular maximal stress testing on the bicycle ergometer and submaximal exercise on the treadmill. The sessions were held three times weekly in a gymnasium over three months, gradually increasing the duration of each session to a maximum of one hour. During the session, heart rate was monitored with a Sport-tester 3000 so as not to exceed 60%, then 70%, of the maximal heart rate recorded during the stress test. Testing at the end of the programme demonstrated an improvement in tolerance under maximal stress in 7 of the 9 patients. As for submaximal performance, 8 out of 9 children covered a greater distance using the same parameters as for initial testing. At the end of the programme, the children all showed increased independence and initiative and more self-confidence in establishing social relations. The programme has proven to be a comprehensive method for safely improving physical fitness in these patients and represents an important starting point for a better future.

Child↗
PubMed1990

[Cycloergometric exercise test in normal children: comparison of an Italian and a North American population].

The need for a more complete protocol for bicycle ergometer exercise testing in children in our country, induced the Authors to apply the F.W. James protocol to healthy Italian children and then compare the results with a study on a similar american sample population. A total of 102 children, 67 males and 35 females, age range 4.4 - 17 years (mean 10.3 yrs), were examined following the F.W. James protocol. The Authors report the anthropometric and ergometric data obtained, the comparison with James' results and the linear correlation coefficients. Normal reference data were obtained. There were no significant differences between the results obtained from the Italian and American groups, and the correlation coefficients were satisfactory. The results will enable us to benefit from a simple and complete protocol which can be applied to healthy and sick children alike in our country.

Adolescent↗
PubMed1989

[Diagnostic usefulness of Holter monitoring in pediatric fainting and syncope].

The aim of the study was to assess the diagnostic efficacy of ambulatory electrocardiographic monitoring in children with a previous history of fainting and/or syncope. Sixty-one children, from 2 months to 17 years of age, all at the time undergoing different physical examinations because of previous syncopal episodes were examined. All the children had a normal electrocardiogram at rest and all underwent 24 hour electrocardiographic monitoring (Holter). The ECG was normal in 48 patients. Eight children had several dysrhythmias, classified as "borderline" and five children showed a clearly pathological trend. These results are even more significant considering that standard electrocardiogram results are negative. These data underline the diagnostic importance of the dynamic electrocardiogram particularly in cases of very frequently repeated symptoms.

Adolescent↗
PubMed1986

[Plasmid content in strains of Azospirillum sp].

In this work, the plasmid content of 22 strains of A. brasilense and 7 of A. lipoferum, isolated from different regions of Argentina was analyzed. Of the several methods for the extraction of plasmids, a modification of that of Kado and Liu was found to be the most appropriate for the isolation of a larger number of high molecular weight plasmids. Out of 29 strains analyzed, 26 were found to contain between one and four plasmids, all of them of high molecular weight (Figure 3, Table 1); 44% of the plasmid-containing strains showed one large plasmid of similar electrophoretic mobility and of approximately 100 Mdal.

Culture Media↗
PubMed1986

[Monitoring cardiac rhythm after the Mustard intervention for transposition of the great arteries].

Before Mustard's operation 27 children, from 2 days to 11 months old, with simple or complex transposition of the great vessels, underwent an electrocardiogram at rest. After operation, all the children underwent a standard electrocardiogram and 24 hours continuous Holter monitoring. Successive electrocardiograms at rest were recorded in all patients, and eleven children were successfully examined by dynamic electrocardiogram. The electrocardiogram at rest showed a sinusal rhythm in 66.7 of the patients; during the follow-up, in 33.3% non sinusal rhythm was recorded. The continuous 24 hours dynamic electrocardiogram, immediately after surgery, revealed 25.9% of the patients with non-sinusal rhythm. After 7 months, the Holter monitoring showed, in one case, a severe sinus node disfunction. Our data and those of the literature confirm the importance to follow these patients during a long period of time, because it was found that, even bedately, can develop serious arrhythmias.

Electrocardiography↗
PubMed1983

Naloxone inhibits exercise-induced release of PRL and GH in athletes.

Opiate peptides stimulate the release of GH and PRL, and such changes have also been reported following physical exercise. To investigate opiate involvement in the exercise-induced release of these hormones, eight professional athletes were exercised to 80% of their maximal heart rate on a bicycle ergometer. This exercise alone induced an increase in circulating mean GH (basal to maximal level, 3.1 +/- 0.9 ng/ml-27.3 +/- 5.9 ng/ml) and mean PRL level (6.1 +/- 1.1 ng/ml-19.5 +/- 1.9 ng/ml). Infusion of naloxone (0.3 mg/min) antagonized these responses in mean serum GH (5.6 +/- 1.0 ng/ml to 8.6 +/- 1.1 ng/ml) and PRL levels (6.4 +/- 1.1 ng/ml-8.1 +/- 1.2 ng/ml), which were both significantly less than during the control infusions (P less than 0.01). It is suggested that certain forms of stress stimulate the release of PRL and GH via endogenous opiate peptides.

Adult↗
PubMed1983

Involvement of a plasmid in Escherichia coli envelope alterations.

A plasmid-containing wild-type Escherichia coli strain was treated with two plasmid-curing agents, sodium dodecyl sulfate and ethidium bromide. Plasmid elimination was accompanied by drastic changes in the morphology of the colonies. Analysis of the cured strain by scanning and transmission electron microscopy showed important alterations in size and morphology of the cells. Metabolic differences were also found between the wild-type and cured cells.

Cell Membrane↗
PubMedDate not supplied

Exercise tolerance and blood pressure response to exercise testing in children and adolescents after renal transplantation.

The aim of the study was to assess exercise tolerance and blood pressure (BP) response to treadmill exercise in children after renal transplantation. Forty-five children were selected (29 males and 16 females) whose mean age was 14.3 +/- 4.2 years. All children had Hb >/= 10 g/dl and creatinine clearance >/=40 ml/min/1.73 m2. They were at least 6 months posttransplantation and were on triple immunosuppressive therapy. Twenty-seven were also on various antihypertensive medications. Each underwent clinical examination and measurement of BP, both at rest and during exercise testing on treadmill. The test was stopped on muscular fatigue or exhaustion. The patients were divided into two groups: those off (A) or on (B) antihypertensive therapy. When compared to a population of healthy children the patients had reduced exercise tolerance (10.1 +/- 2.1 vs 15.1 +/- 1.7 min, p < 0.001) (67 +/- 16%), increased heart rate (174 +/- 19 vs 161 +/- 19 beats/min, p < 0.001) (109 +/- 15%), and increased maximum systolic BP (150 +/- 26 vs 134 +/- 13 mmHg, p < 0. 001) (113 +/- 19%) at comparable workloads. Within the two patient groups, significant differences were observed during exercise testing for maximum heart rate, which was lower in group B (p = 0.03), and maximum systolic BP, which was higher in group A (p = 0.04). Our study confirms that children and adolescents on immunosuppressive therapy after renal transplantation have a hypertensive response during exercise, probably related to medication-induced peripheral vascular tone.

Adolescent↗