Biomedical subjects
A C Stowe
Publications and source records attributed to A C Stowe.
Effect of ethanol on alkaline phosphatase activity in HeLa cells.
Incubation of HeLa cells with 10-80 mM ethanol, caused a dose-dependent decrease in alkaline phosphatase specific activity. With 80 mM ethanol, the decrease became apparent after 24-48 hr incubation. The same dose-dependent effect was observed with the nonmetabolizable alcohol t-butanol. The effect of ethanol was not due to toxicity as the cells continued to replicate for weeks in the presence of ethanol and was reversible. There were no differences in the Km values of alkaline phosphatase in crude extracts or solubilized enzyme prepared either from control or 80 mM ethanol-treated cells. Hydrocortisone and choline chloride, which by themselves increase alkaline phosphatase specific activity, altered the effect of ethanol on alkaline phosphatase. In the presence of either 1 microM hydrocortisone or 40 mM choline chloride, neither 10 nor 20 mM ethanol decreased alkaline phosphatase specific activity.
Learning from literature: novels, plays, short stories, and poems in nursing education.
Over the last several years, there has been a growing awareness of a need for better integration of the liberal arts into nursing curricula. To facilitate this process, the authors describe a creative teaching strategy and offer specific suggestions for the selection and use of novels, short stories, plays, and poems to supplement the teaching of specific nursing content.