The apocytochrome b genes from two strains of the yeast Kluyveromyces lactis, have been isolated and sequenced. The coding sequences in strains WM27 (NRRL Y-17066) and WM37 (NRRL Y-1140) were identical but the upstream noncoding regions were slightly different. The sequences demonstrated the presence of a continuous open reading frame with no introns. The amino acid sequence, derived from the coding strand, showed 82% homology to the apocytochrome b of Saccharomyces cerevisiae strain D273-10B and only 58% homology to the protein from Schizosaccharomyces pombe strain 50. CUN and CGN codon families were absent from the K. lactis gene. Codon usage was very similar to that of other mitochondrial genomes with mostly U or A in the third position. There were two unusual features. All threonines were coded by ACA(U) and all arginines by AGA.

Amino Acid Sequence↗

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[Surgical treatment and prognosis factors in spinal metastases of breast cancer].

AIM: The aim of this study was the evaluation of surgical therapy results and prognosis factors in patients with spinal metastases of breast cancer. METHODS: 55 patients with spinal metastases of breast cancer who were treated surgically were retrospectively evaluated. In 11 patients the cervical, in 27 patients the thoracic and in 17 patients the lumbar spine was affected. RESULTS: Postoperatively, 45 patients (81.8 %) described a reduction in pain and 5 patients (50 %) reported a neurological improvement. Perioperative complications appeared in 27 patients (49.1 %), 2 patients died. For the entire group, the mean postoperative survival was 27.2 +/- 28.6 months and the median survival 16.2 months. In patients with solitary metastasis the univariate analysis did not show a significantly longer postoperative survival than in patients with additional visceral metastases (p = 0.0659), but patients with solitary metastasis showed a significantly longer survival than those with multiple osseous and/or visceral metastases (p = 0.0325). In the univariate analysis, the classification of the primary tumour, the duration of symptoms, the localisation of the metastases, the patient's age and the kind of surgical procedure (posterior stabilising instrumentation versus combined posterior-anterior treatment with intralesional resection of the affected vertebra and vertebral body replacement) did not show a significant influence on the postoperative survival. The multivariate analysis did not show a significant prognostic influence for the potentially prognostic factors, however, solitary and multiple metastasis showed the highest statistical influence for the prognosis (p = 0.1187), followed by the classification of the primary tumour (p = 0.1243). CONCLUSION: Pain reduction and neurological improvement can be reached by a stabilisation of the diseased spinal region. Patients with spinal metastases due to breast cancer showed a relatively long postoperative median and mean survival. Therefore, the preoperative evaluation of extent of the disease and the therapy concept should be individually adapted. The surgical procedure (posterior stabilising instrumentation versus combined posterior-anterior approach with vertebrectomy and vertebral body replacement) does not significantly influence the survival.

Adult↗

PubMedDate not supplied

[Total hip arthroplasty in congenital dysplasia of the hip: follow-up of a small-dimensioned, cemented straight stem].

AIM: The aim of this study is to report the survival and mid-term results including patient-relevant outcome measures with a small-sized cemented stem (CDH-stem) for the treatment of coxarthrosis secondary to developmental dysplasia of the hip. METHOD: 33 total hip replacements were performed with the CDH stem, 29 of which (88 %) were included in the follow-up after an average of 7.6 years. According to the classification system of Hartofilakidis et al., 10 hips were classified as type 1, 15 as type 2, and 3 as type 3. Survival was predicted using Kaplan-Meier survivorship analysis with revision as the end point. Results were assessed using the Harris hip score, as well as the WOMAC and SF-36 as patient-relevant outcome measures. RESULTS: Survival at thirteen years was predicted to be 92.4 % for the stem and 87.6 % for the varying acetabular implants used. At the time of the final follow-up, the average Harris hip score was 82.45 points. The global WOMAC index averaged 2.1, the average SF-36 score was 66.2 points. CONCLUSIONS: The data support the use of a small-sized cemented stem in small femora for total replacement of the dysplastic hip. To the best of our knowledge, this is the first study utilising the well validated WOMAC and SF-36 as patient-relevant outcome measures in this subgroup of patients.

Aged↗

PubMedDate not supplied

1,25-Dihydroxyvitamin D3 affects the synthesis, phosphorylation and in vitro calmodulin binding of myoblast cytoskeletal proteins.

Incubation of chick embryo myoblasts with 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] (10(-10) M, 24 h), markedly stimulated the incorporation of [3H]leucine into total cytoskeletal proteins and this effect was abolished when the sterol treatment was performed in the presence of cycloheximide or actinomycin D. 1,25(OH)2D3 selectively stimulated the de novo synthesis of several proteins with apparent molecular masses (isoelectric points) of 220 kDa (6.1 and 9.7), 150 kDa (7.5), 110 kDa (7.2), 68 kDa (9.5, 7.5 and 4.5), 50 kDa (8.5), 44 kDa (6.3), 27 kDa (7.8) and 15 kDa (5.5). Labelling of proteins with [125I]calmodulin after their separation on SDS-polyacrylamide gels showed that 1,25(OH)2D3-dependent protein of 110 kDa is the major calmodulin-binding component of myoblasts cytoskeleton. In addition, the sterol increased the phosphorylation of several cytoskeletal proteins including that of 110 and 15 kDa whose synthesis potentiates.

Animals↗

PubMedDate not supplied

Cytomorphologic findings on the final stages of erythrocyte maturation in mamals.

An increase of organelles occurs at the reticulocytary maturation stage, when compared with the erythroblastic one, in the peripheral blood of embryos and of patients with acquired hemolytic anemia. This increase is due to hemosome formation, in which possibly the final stage of hemoglobin biosynthesis may take place. In animals with posthemorrhagic anemia, biosynthesis does not occur and reticulocytes present only typical mitochondria. Based on these findings, as well as on reticulocyte basophilia, suggestions were done for the introduction of some modifications in the Harris & Kellermeyer16 1970 conception of the erythrocyte maturation.

Animals↗

PubMedDate not supplied

[On the presence of intracytoplasmic epithelium fibrils of the cells of the human conjunctiva. Electrom microscopic study].

Explore the source record for details and available documents.

Conjunctiva↗

PubMedDate not supplied

[HELLP syndrome in routine obstetrical care. Three case reports].

In recent years the syndrome of hemolysis, elevated liver enzymes and low platelets (H-ELLP) has attracted increasing interest in obstetrics as a serious complication of pregnancy, either alone or in combination with the classical symptoms of EPH-gestosis or eclampsia. In 1993, we observed 3 cases of severe HELLP syndrome in a total of 1126 deliveries. We present the clinical characteristics and the laboratory findings in these cases. A common symptom was general malaise and upper abdominal discomfort or pain. All patients were delivered by cesarean section of healthy infants. We conclude that it is no longer sufficient to emphasize edema, proteinuria and hypertension, but that the signs and symptoms of the HELLP syndrome present a new and increasingly important challenge in obstetric practice.

Adult↗