Search PubMed⌕ Search

Biomedical subjects

A Brun

Publications and source records attributed to A Brun.

At least 181 records · Page 10Linked to original sources
PubMed1982

Infectious bovine rhinotracheitis: study on the experimentally induced disease and its prevention using an inactivated, adjuvanted vaccine.

Experimentally induced IBR was studied in calves. Intranasal challenge enabled reproducible results to be obtained, both from qualitative (clinical aspect) and quantitative points of view (virus excretion, temperature); local and general immunity were also evaluated. This challenge method is useful when studying IBR vaccines. The disease was also experimentally induced by putting healthy animals into contact with diffusor calves. A single injection of inactivated vaccine in oily adjuvant already conferred good protection; it was 100% successful against the experimentally induced disease when administered two times at a 7 or 14 day interval. Immunity obtained was long-lasting and even persisted up to one year. Therefore, this vaccine is advised for vaccination in both contaminated and high risk areas. Results obtained for both safety and potency suggest that this killed vaccine should be used rather than live vaccines.

Adjuvants, Immunologic↗
PubMed1982

Senile cerebral amyloid. Prealbumin as a common constituent in the neuritic plaque, in the neurofibrillary tangle, and in the microangiopathic lesion.

Three lesions that characterize the nosologic findings in the brain of Alzheimer's presenile dementia and senile dementia of Alzheimer type, ie, neuritic plaque, neurofibrillary tangle, and microangiopathy, all are frequently associated with amyloid deposition. There has been some question, however, as to whether these lesions share the same etiology. Moreover, the specific chemical nature of amyloid associated with these lesions has not yet been determined. In the present study, formalin-fixed paraffin sections of the affected brains were tested immunocytochemically for their reactivity against antiserum to prealbumin (recently disclosed as the major constituent of amyloid associated with familial amyloidotic polyneuropathy as well as senile cardiac amyloid) and known components of other types of amyloid (AA, AP, etc.). The results demonstrated that amyloid deposits in all three lesions reacted with anti-prealbumin, suggesting that it is a common constituent of these lesions. Indeed, it is likely that prealbumin is the major constituent of amyloid associated with neuritic plaque, neurofibrillary tangle, and microangiopathy.

Alzheimer Disease↗
PubMed1982

Immunohistochemical evidence for the lack of amyloid P component in some intracerebral amyloids.

Sections of brain tissue from patients with Alzheimer's disease with amyloid deposits in vessels, in plaques, and within nerve cells were studied by means of an immunoperoxidase method using a specific antiamyloid P component (AP) antiserum. Amyloid deposits in vessels were found to be strongly positive for protein AP, whereas the Alzheimer's plaques and the neurofibrillary tangles were negative or only weakly positive. The absence of protein AP in some intracerebral amyloid deposits might be due to an inability of the protein AP in some intracerebral amyloid deposits might be due to an inability of the protein to penetrate the blood-brain barrier. These findings support the theory that protein AP is absorbed to already formed amyloid fibrils, but the possibility that it may participate in the formation of vascular amyloid cannot be dismissed. The findings also indicate that protein AP is not necessary for the formation of amyloid fibrils at least in some forms of cerebral amyloidosis.

Alzheimer Disease↗
PubMed1982

Sideroblastic anemia with markedly increased free erythrocyte protoporphyrin without dermal photosensitivity.

A patient with idiopathic sideroblastic anemia and atypical clinical and biochemical findings is described. He had a greatly increased erythrocyte and plasma protoporphyrin, but normal urine and fecal porphyrins. The erythrocyte protoporphyrin had a fluorescence spectrum typical of free protoporphyrin, but caused no photosensitivity. Bone marrow metal chelatase activity was normal. There were no clinical signs of liver disease. The abnormal porphyrin metabolism in this patient is not known though a number of explanations are discussed.

Aged↗
PubMed1981

Combined modality therapy of operated astrocytomas grade III and IV. Confirmation of the value of postoperative irradiation and lack of potentiation of bleomycin on survival time: a prospective multicenter trial of the Scandinavian Glioblastoma Study Group.

In a controlled, prospective, randomized investigation, started in 1974, 118 patients with supratentorial astrocytoma Grade III--IV were divided into three groups. Groups 1 and 2 received 45 Gy postoperatively to the whole supratentorial brain. Bleomycin in 15-mg doses and a total dose of 180 mg or placebo was given intravenously three times a week, one hour prior to radiotherapy, during weeks 1, 2, 4 and 5. Group 3 received conventional care but no radiotherapy or chemotherapy. Median survival rates of patients were 10.8 months in Groups 1 and 2, and 5.2 months in Groups 3, a statistically significant difference. With regard to performance, the patients in Group 3 deteriorated faster than patients in Groups 1 and 2. Bleomycin had no positive or negative influence on survival.

Adult↗
PubMed1981

Regional pattern of degeneration in Alzheimer's disease: neuronal loss and histopathological grading.

The various structural components of the cortical degeneration of Alzheimer's disease were defined and graded. The severity of the degenerative process was thus mapped in different cortical areas where neuronal numbers and cortical width were also measured and compared with controls. Contrary to the general opinion that the degenerative process is rather uniformly diffuse, though accentuated in the medial temporal and frontal cortex, we found a consistent and more elaborate pattern with clearcut regional differences. Thus the degeneration involved, besides basal medial temporal limbic areas, the posterior cingulate gyrus and superior parietal lobule particularly, with somewhat less marked changes in the inferior parietal lobule and inferior temporal gyri. The sensorimotor, calcarine and anterior cingulate areas of the cortex were notably spared until very late stages. This regionally variable severity of the degeneration was also paralleled by a regionally varying reduction in neuronal numbers and cortical width, and agrees with our previously published results of a regional pattern which closely correlates with clinical parameters, including symptom pattern and regional cerebral blood flow alterations.

Aged↗
PubMed1981

Senile plaques and tangles in dialysis dementia.

The brains of 7 patients treated with hemodialysis were studied. Four of these patients had the dialysis encephalopathy syndrome (DES). Senile plaques and or neurofibrillary tangles were found in 5 of the 7 cases, 3 with and 2 without DES. One case of each group had plaques to an extent compatible with that in Alzheimer's disease, though of a different distribution. Neurofibrillary tangles were generally sparse. Plaques and tangles, both containing paired helical filaments, are the principal changes in Alzheimer's disease. Aluminum has been implicated as a possible etiologic agent behind the paired helical filaments in Alzheimer's disease, where raised cerebral aluminum contents have been demonstrated. Aluminum is known to be increased in the central nervous system also in DES, most likely as a consequence of procedures in connection with the hemodialysis. The finding of neurofibrillary tangles and senile plaques in hemodialyzed patients, some of which have developed DES, may therefore support the theories concerning aluminum as an etiological agent for cerebral changes in Alzheimer's disease.

Brain↗
PubMed1981

Experimental rabies in cats: immune response and persistence of immunity.

Experimental rabies was studied in cats under defined conditions. A correlation between the virus dosage and the incubation period was established and results were compared with those published previously by others. the susceptibility of cats to rabies virus appears to be paradoxical. The persistence of the immunity induced by an inactivated rabies vaccine prepared form cell culture-propagated virus used in combination with feline panleukopenia vaccine, with or without adjuvant, was studied through severe virulent virus challenge. Complete protection was observed after more than 3 years following a single vaccination. A proposed vaccination schedule is discussed.

Animals↗
PubMed1980

Recurrent meningoencephalitis and ascending myelitis caused by dermal sinus tract of extraordinary length.

The case of an infant with a dermal sinus tract, recurrent meningitis, ascending myelitis and a fatal outcome is described. The extraordinary extent of the cord affection with abscesses up to the level of the lower brainstem, and the presence of squamous epithelium within them, is difficult to explain on the basis of current concepts of the formation of dysraphic dermal sinus tracts. Morphogenetic, clinical and radiological aspects are discussed.

Humans↗
PubMed1980

A flow-quench apparatus for cryoenzymic studies. Application to the creatine kinase reaction.

The flow-quench method was adapted to sub-zero conditions. Two apparatus were constructed: a rapid flow-quench apparatus (which take samples in the 5--300-ms time range) and a time delay flow-quench apparatus (0.5 s and longer). The apparatus were constructed so that the reagents are only in contact with chemically inert materials : glass, poly(tetrafluoroethylene) and poly(trifluorochloroethylene). The modified flow-quench apparatus were used to study the initial formation of creatine phosphate by creatine kinase at -15 degrees C in the time range 5 ms to 10 s. As at +4 degrees C [Travers, F., Barman, T. E. and Bertrand, R. (1979) Eur. J. Biochem. 100, 149--155], the time course of product formation was complex and consisted of three phase: a lag phase, a burst phase and the steady-state phase. The apparatus were also used to test chemically for reaction intermediates involving labile phosphate and phospho-enzyme complexes on the creatine kinase reaction pathway at -15 degrees C. Since neither type of intermediate could be detected down to 5 ms, this enzyme probably proceeds via a direct in-line type of mechanism.

Animals↗
PubMed1980

Effect of zinc on protoporphyrin induced photohaemolysis.

Zinc is readily inserted into protoporphyrin suspended either in a salt-sucrose buffered solution or endogenously present in red cell haemolysate in erythropoietic protoporphyria. Red cells from healthy persons were incubated with metal-free protoporphyrin or zinc-protoporphyrin and irradiated with light at 360-470 nm. The photohaemolysis was significantly less with zinc-protoporphyrin compared with that of the metal-free protoporphyrin. The results are discusssed in relation to the possibility of using zinc as a photoprotective and hepatoprotective agent in erythropoietic protoporphyria.

Animals↗