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Biomedical subjects

A Brock

Publications and source records attributed to A Brock.

At least 73 records · Page 4Linked to original sources
PubMed1987

Amylase in duodenal aspirates: relationship between concentration, activity and isoenzyme pattern.

Concentrations of immunoreactive amylase, catalytic activities, and isoenzyme patterns were compared in 220 duodenal aspirates from 205 patients suspected of pancreatic disease. Immunoreactive amylase was measured in an ELISA assay, employing rabbit antibodies raised against a highly purified human pancreatic amylase. Generally, the 220 duodenal aspirates showed a linear relationship between catalytic activity and concentration. Twenty-five of the 220 samples contained the slowly migrating form of pancreatic amylase, caused by heterozygosis of the Amy-2 locus. This phenomenon did not influence the relation considered. Thirty-two of the 220 aspirates contained more than 20% non-pancreatic amylase. The concentration of immunoreactive amylase in these samples corresponded to that expected from their isoenzyme composition.

Amylases↗
PubMed1987

Amylase in serum of healthy subjects. The relationship between catalytic concentration and substance concentration is not effected by the isoenzyme pattern.

Catalytic activity concentrations and substance concentrations of immunoreactive amylase were measured in serum samples from 140 healthy subjects with various electrophoretic isoenzyme patterns. The relationship between catalytic activity concentrations and corresponding substance concentrations of immunoreactive amylase was independent of the actual amylase isoenzyme pattern.

Amylases↗
PubMed1981

Disseminated intravascular coagulation, antithrombin III, and complement in meningococcal infections.

Serial assessments of some blood coagulation factors, antithrombin III (AT III), and complement were made in 18 patients with meningococcal (mgc) infection. All patients displayed laboratory evidence of activation of the blood clotting system. Two patients showed clinical signs of disseminated intravascular coagulation. Only AT III differed significantly between patients with and without complications. There was no correlation between changes in blood clotting, activation of the complement system and the course or duration of the disease. These results do not enable one t identify patients who need specific prophylactic therapy. Controlled clinical trials, including administration of heparin, dextran, aprotinin, and others, are still required to ensure optimal treatment in fulminant mgc infections.

Adolescent↗
PubMed1979

The renal handling of amylase in normal man.

The renal glomerular and tubular transport rate of amylase was studied by measuring the urinary excretion of this protein before and during inhibition of tubular protein reabsorption by lysine. The excretion of amylase was compared with the excretion of albumin, beta-2 microglobulin and free light chains of immunoglobulins. This investigation showed that amylase is reabsorbed by the tubular cells, but only to a very modest degree compared with the reabsorption of the other three proteins. In the case of amylase only about 45% of the filtered molecules are reabsorbed, whereas more than 90% of the filtered amount of the other molecules is reabsorbed by the tubular cells. The excretion of amylase rose after lysine injection only by a factor 1.8, whereas excretion rose by a factor 28 for albumin, 1,500 for beta-2 microglobulin, 16 for kappa chains and 8 for lambda chains. Minimal values for tubular reabsorption were found to be 5.5 +/- (SD) 4.3 U/min for amylase, 174.0 +/- 35.7 micrograms/min for albumin, 90.5 +/- 14.4 micrograms/min for beta-2-microglobulin, 70.4 +/- 17.4 micrograms/min for kappa chains and 24.2 +/- 9.2 micrograms/min for lambda chains.

Adult↗
PubMed1978

Hepatitis B virus infections among Danish dentists.

Since type B hepatitis is generally regarded as an occupational risk for dentists, the participants at the 1976 annual meeting of the Danish Dental Association were examined for hepatitis B surface antigen (HBsAg) and antibody to HBsAg (anti-HBs). A total of 1,338 dentists (89% of the dentists at the meeting and 29% of all Danish dentists) were included in the study by completion of a questionnaire and by radioimmunoassay of a blood sample for HGsAg and anti-HBs. None of the dentists was HBsAg-positive, but 110 (8.2%) had anti-HBs. An increasing frequency of anti-HBs was found with increasing age, but the figures were similar to the findings in a control population. Evidence is presented that hepatitis found before admittance to or during the time at dental school was predominantly not of type B. In contrast, type B hepatitis predominated during the professional activity of the dentists. On the basis of the serological findings in 29% of all Danish dentists, it is concluded that dentists cannot be regarded as a high-risk group for hepatitis B.

Aging↗
PubMed1977

Peroxidase activity in human granulocytes, inter- and intra-individual variations.

A kinetic method for quantitation of peroxidase activity ('myeloperoxidase', E.C. 1.11.1.7.) in human granulocytes has been evaluated. The enzymic activity was determined at 37 degrees C, PH = 7.0. Substrates: 2-methyxo-phenol (1.25 mmol/l) and H2O2 (0.1 mmol/l). When applied on granulocytes from solitary blood samples from healthy, normal subjects the detected peroxidase activity varied considerably from one person to another (CV = 40-50%). Sequential determinations of the peroxidase activity in granulocytes from selected healthy, normal subjects demonstrated substantial intra-individual variations. Analysis of variance confirmed that the intra-individual variations (CV = 36%) exceeded all other variance components involved.

Adult↗
PubMed1976

Serum amylase activity and renal amylase activity clearance in patients with severely impaired renal function and in patients treated with renal allotransplantation.

Serum amylase activity was measured in 29 nondialysed patients with severe renal failure, in 24 uraemic patients treated with chronic haemodialysis, and in 29 patients treated with renal allotransplantation. Simultaneous measurement of renal amylase activity clearance (CAm) and creatinine clearance (CCr) was performed in 25 patients with severe renal failure and in 19 transplanted patients. Serum amylase activity was elevated in all three groups. CAm was significantly correlated to CCr both in the group with severe renal failure and in the transplanted group. Unlike in the group of transplanted patients, the ratio CAm/CCr was significantly increased in patients with severe impaired renal function. It is concluded that the elevation of serum amylase activity in patients with impaired renal function is primarily due to decreased glomerular filtration rate. The value of CAm/CCr for diagnosing acute pancreatitis is doubtful in patients with severe renal disease.

Amylases↗