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A Boudreault

Publications and source records attributed to A Boudreault.

47 records · Page 3Linked to original sources
PubMed1967

An epidemic of influenza B in the province of Quebec in the Spring of 1966: laboratory studies.

Laboratory studies of an influenza B epidemic in the Province of Quebec carried out during the spring of 1966 are described. During that period six influenza B strains were isolated. The isolated strains did not differ markedly from previous influenza B strains. The serological studies showed that this epidemic had spread over different parts of the Province of Quebec.The serological studies also showed that an isolated community, such as an armed forces camp, can exhibit an epidemiological pattern different from that obtaining in the whole population.

Complement Fixation Tests↗
PubMedDate not supplied

Application of the multiple antigenic peptides (MAP) strategy to the production of prohormone convertases antibodies: synthesis, characterization and use of 8-branched immunogenic peptides.

Antiserum against an N-terminal sequence of murine prohormone convertase-1 (mPC1) incorporating the sequence immediately following the junction between the putative pro-region and the active enzyme was obtained. This was accomplished using the multiple antigenic peptide (MAP) approach whereupon an 8-branched polylysine core to which are grafted multiple copies of a 16 amino acid peptide representing the N-terminal sequence of mPC1 (positions 84-99) was synthesized by solid-phase Fmoc chemistry. The ensuing peptide was purified and fully characterized by RP-HPLC, 1H-NMR, amino acid composition, peptide sequencing and ion-spray mass spectrometry. The immunological properties of the resulting antibodies in detecting recombinant PC1 in both crude and purified preparations were compared with antibodies raised against a similar N-terminal segment of PC1 but using the conventional method of peptide-carrier protein conjugation and also developed against a C-terminal fusion protein of PC1. Our data indicate that the MAP antibody was as efficient as both the amino and carboxy-terminal antibodies in qualitative as well as quantitative analysis of PC1 encoded protein by radioimmunoassay. Following an identical approach, antibodies against other prohormone convertases like furin, PC5/6 and PACE4 were also developed and subsequently applied to a number of biochemical and immunological studies. In each case, the case of preparation and high immunogenicity of the MAP approach were confirmed and reside in the simplicity and rapidity with which a potent and useful antiserum is obtained.

Amino Acid Sequence↗
PubMedDate not supplied

In vitro markers for measuring residual virulence for men of live attenuated influenza viruses.

Human and equine influenza strains attenuated by consecutive passages in the presence of normal horse serum (NHS) on allantois-on-shell system (AOS) and administered to human volunteers or horses as possible live virus vaccine candidates were tested in organ cultures of ferret and hamster trachea. Temperature sensitivity, virus replication, interferon induction and neuraminidase activity were also investigated. Observation of the ciliary activity of each ring was made daily. Influenza strains which were incompletely attenuated following two to five passages on AOS + NHS system and caused severe to mild symptoms of influenza in volunteers or horses also caused a 50% inhibition of the activity of the ciliated epithelium earlier than did completely attenuated strains following ten passages on the same system. The A/Hong Kong/68 strain attenuated by Beare and Bynoe was used as a reference strain in every test. This technique appears to be valuable for screening live influenza vaccine candidates and may prevent risking severe illness in volunteers. No significant results could be ascertained with other markers investigated.

Animals↗