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Biomedical subjects

A Bhaduri

Publications and source records attributed to A Bhaduri.

86 records · Page 5Linked to original sources
PubMed1975

UDPGlucose 4-epimerase from Saccharomyces fragilis. Allosteric kinetics with UDP-glucose as substrate.

UDPglucose 4-epimerase from Saccharomyces fragilis catalyzes a freely reversible reaction between UDP-galactose and UDP-glucose. With UDP-galactose as the substrate the enzyme shows a classical hyperbolic kinetics but when UDP-glucose is used as the substrate a distinct allostericity is observed. As a consequence, at low concentrations of UDP-glucose, the enzyme fails to establish the equilibrium at a significant rate. Glucose 6-phosphate acts as a strong activator for the enzyme with low concentrations of UDP-glucose as the substrate. In view of these rather unusual kinetic data for an enzyme catalyzing a freely reversible reaction, UDPglucose 4-epimerase may play a regulatory role in controlling the flux of galactose metabolism.

Allosteric Regulation↗
PubMed1975

Galactose-6-phosphate dehydrogenase. Purification and partial characterization.

A new enzyme, galactose-6-phosphate dehydrogenase has been purified about 50-fold from goat liver. The enzyme can be distinguished from the nonspecific hexose-6-phosphate dehydrogenase and glucose-6-phosphate dehydrogenase by its high substrate specificity and absolute pyridine nucleotide requirement. In contrast to the hexose-6-phosphate dehydrogenase, this enzyme is located exclusively in the cytoplasmic fraction of the cell. The enzyme is a metalloprotein and is highly sensitive to mercurials. The product of the reaction is possibly a ketoaldose, phosphorylated at the primary alcoholic group.

Alcohol Oxidoreductases↗
PubMedDate not supplied

Development of secondary sex characteristics in multitransfused thalassemic children.

Secondary sex characteristics were evaluated in thalassemic children (41, boys and 30 girls), who were on regular transfusion therapy and were above 11 years of age. The results of this study indicate that the development of secondary sex characteristics in thalassemic children is markedly delayed as compared to their non-thalassemic siblings and to the expected development criteria. Delay in development of secondary sex characteristics appears to be secondary to chronic hypoxia and iron overload.

Adolescent↗
PubMedDate not supplied

Physical growth parameters in thalassemic children.

Various growth parameters of 233 (140 boys, 93 girls) thalassemic children were compared with 74 (45 boys and 29 girls) non-thalassemic siblings, ICMR and NCHS norms. Weight and height were retarded in thalassemic children. The difference between thalassemic and non-thalassemic siblings, was evident from 9+ years in both boys and girls. Growth parameters seemed to be adversely affected with the advancing age. Among head, chest and mid arm circumferences, the mid arm circumference was more affected than head and chest circumferences. Height and weight were more severely retarded in children with hemoglobin levels of less than 8 gm/dl. The findings seemed to suggest that press of retardation was probably secondary to chronic hypoxia and iron overload.

Adolescent↗
PubMedDate not supplied

Lymphocytic interstitial pneumonitis associated with autoimmune hepatitis.

A 49-year-old woman was diagnosed as autoimmune hepatitis and started on steroids and azathioprine. Subsequently, she developed fever; chest radiograph showed lower lobe nodular opacities. Bronchoalveolar lavage and transbronchial lung biopsy confirmed the diagnosis of lymphocytic interstitial pneumonitis.

Adrenal Cortex Hormones↗
PubMedDate not supplied

Herpes simplex esophagitis in immunocompetent individuals.

Herpes simplex esophagitis commonly occurs in immune-compromised individuals. We report the condition in two immunocompetent individuals (one presenting with retrosternal pain and diarrhea and the other with dysphagia and fever) and in two patients with obstructive airway disease who had received corticosteroid therapy. The first two did not receive treatment, one was lost to follow up and the other is asymptomatic two years later. The latter two patients received acyclovir therapy.

Adrenal Cortex Hormones↗
PubMedDate not supplied

Metabolic analysis of galactose toxicity in Escherichia coli with 2-deoxygalactose as the probe.

Biochemical basis of galactose toxicity has been studied in gal T mutants (CGSC 4974) using 2-deoxygalactose, a non-metabolizable analogue of galactose, as the probe. It is found that biochemical features of toxicity in wild type cells either with 2-deoxygalactose or with 2-deoxyglucose are very similar to the picture obtained with gal T mutants and the observed bacteriostasis is probably due to futile phosphorylation and not due to any specific inhibitory effect of phosphorylated galactose.

Escherichia coli↗