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Biomedical subjects

A Bernard

Publications and source records attributed to A Bernard.

At least 595 records · Page 33Linked to original sources
PubMed1981

[Burkitt's type acute lymphoblastic leukemia. Cellular phenotype and chromosome abnormally (author's transl)].

A 13 year-old boy presented with Burkitt's type acute lymphoblastic leukemia (B-ALL). Studies of immune markers on his lymphoblasts suggest they have reached a fairly advanced level of differentiation with in the B-cell lineage. Presence of 1q+ and 8q- chromosomes suggest an unusual (1;8) translocation and is in agreement with current concepts concerning the frequent chromosome 8 changes in B-ALL.

Adolescent↗
PubMed1981

Surface antigens on malignant Sézary and T-CLL cells correspond to those of mature T cells.

Tumor cells from eight adult patients with T-cell chronic malignancies were investigated with a series of monoclonal antibodies recognizing T-cell differentiation antigens. This series allowed definition of discrete subpopulations of mature T cells with functional specialization. All six patients with Sézary syndrome and one patient with T-chronic lymphocytic leukemia had cells with the same phenotype as normal helper/inducer T cells, whereas the other patient with T-chronic lymphocytic leukemia had cell with the same phenotype as normal cytotoxic/suppressor T cells. Some clinical manifestations observed in these patients may reflect retention of functional activities by their malignant cells.

Antibody Specificity↗
PubMed1981

Assessment of renal function of workers simultaneously exposed to inorganic lead and cadmium.

The renal function of a group of workers (n = 62) exposed simultaneously to lead and to cadmium has been examined. The results were compared with those obtained in a earlier study of three groups of workers - one exposed to lead only, one exposed to cadmium only and one not exposed to either of these metals (control group). The results obtained in the earlier study have been published previously. No interaction between lead and cadmium is evidenced. The signs of renal dysfunction found in the group exposed simultaneously to lead and to cadmium can be ascribed to cadmium only. The results of this study have confirmed the authors' previous observations, that is, a moderate exposure to lead (plumbemia less than 62 micrograms/100 ml and average duration of exposure = 13.2 years) does not seem to influence renal function; in adult male workers the critical levels of cadmium in blood and in urine are 1 microgram/100 ml whole blood and 10 micrograms/g creatinine, respectively; the renal dysfunction induced by cadmium is both glomerular and tubular.

Adult↗
PubMed1981

[Results of treatment of 178 pediatric non Hodgkin's malignant lymphomas between 1973 and 1978 (author's transl)].

178 children presenting with non Hodgkin's lymphomas (NHL) were treated using the same protocol from 1973 to 1978 at the Institut Gustave-Roussy. They were classified according to stages (13 stage I, 21 stage II, 80 stage III, 50 stage IV, 6 undetermined stage), according to initial site of involvement (abdomen: 68, mediastineum 55, ORL: 32, lymph glands: 13, "others": 10) and according to histology. They were given combination chemotherapy (Vincristinee-adriamycin-cyclophosphamide-prednisone), preventive CNS therapy by cranial irradiation and intrathecal methotrexate (except in stage I patients) and maintenance therapy by cyclic multiple agents regimens including vincristin-adriamycin, vincristin-cyclophosphamide and cytarabine-asparaginase. Radiation therapy was carried out up to 1977 in stage I and II patients only. Combination chemotherapy improved global survival of pediatric NHL (40% at 4 years, all staged added); however improvements remain to be done in patients with widespread disease.

Adolescent↗
PubMed1981

Effect of progesterone on the post partum rat uterus.

The reactivity of the uterus to estradiol (E2) and progesterone (P) has been examined in 44 post partum rats. Hormone treatment was initiated after surgical delivery at approximately 24 hours before the expected onset of labor in 10 animals and immediately after spontaneous labor in 34 rats. At the time of steroid treatment each animal was equipped with a pressure sensor for the sequential recording of intrauterine pressure (IUP) before and after the oxytocin test. Daily measurements during one week showed that post partum the IUP increases significantly in all animals not treated with P. The combined administration of E2 + P significantly reduces the maximum IUP (IUPm) if treatment is initiated approximately 24 hours before the spontaneous onset of labor. P treatment alone, initiated after spontaneous delivery, is ineffective, but it suppresses IUPm significantly if given in combination with E2 and during 3 days or longer. Apparently, shortly before and during labor the reactivity of the rat uterus to P changes markedly, a process which is inadequately understood.

Animals↗
PubMed1980

An antigen shared by a human T cell subset and B cell chronic lymphocytic leukemic cells. Distribution on normal and malignant lymphoid cells.

We obtained a monoclonal antibody, A50, after immunizing Biozzi's high responder strain of mice with T cell chronic lymphocytic leukemia (T-CLL) cells. A50 recognized an antigen present on the surface of B cell chronic lymphocytic leukemia cells from many patients and from cells of T lineage from any subject we tested. We could not find this antigen either on the surface of normal B cell or on other non-T cell malignancies. On T cells, this antigen was present on a subpopulation of thymus cells, and on most peripheral T cells. The antigen was present on the surface of cells from T-CLL, Sézary's disease, and a subset o T cell lymphoma. The antigen seemed to belong to a complex set of antigenic determinants that we had defined with rabbit antisera.

Animals↗
PubMed1980

[Non-Hodgkin's malignant lymphoma: reliability of "typing" using cyto-enzymatic markers. Comparison with immunological markers (author's transl)].

Comparison between membrane markers and enzyme markers was made in 74 cases of non-Hodgkin's malignant lymphomas and a good correlation appears between both methods in order to distinct lymphomas into T and B origin. Enzyme markers are reliable and provide quickly made and easily interpretable documents. As far as T-lymphomas are concerned, three hydrolases namely acid phosphates e, acid esterase and B-glucuronidase give the same good results. As for B-lymphomas, a specific enzyme marker has to be found. Furthermore, typing of malignant lymphomas by enzymatic and/or immunologic methods appears to be quite better than from morphologic features such as convoluted or cleaved nuclei for example.

Acid Phosphatase↗
PubMed1980

Relationship between exposure to heavy metals and prevalence of renal dysfunction.

Total proteinuria (biuret method) and the urinary excretion of specific proteins (albumin, transferrin, orosomucoid and IgG measured by an automated immunoprecipitin reaction and beta 2-microglobulin determined by radioimmunoassay) have been assessed in four groups of workers: a control group (n = 88) and a group exposed to cadmium (n = 148), mercury vapour (n = 63) and lead (n = 25) respectively. The results demonstrate that a moderate exposure to lead (lead concentration in blood < 62 micrograms/100 ml) does not change the prevalence of renal dysfunction, whereas mercury vapour exposure increases the prevalence of subjects with excessive excretion of albumin (> 12 mg/g creatinine), orosomucoid (> 4.35 mg/g creat.) and IgG (> 3.5 mg/g creat.). Besides an increased excretion of beta 2-microglobulin (> 200 micrograms/g creat.) workers exposed to cadmium excrete a greater amount of high molecular weight proteins. An increased excretion of urinary proteins occurs when mercury and cadmium level in urine exceeds 50 and 10 micrograms/g creatinine respectively.

Cadmium Poisoning↗
PubMed1980

High efficiency of Biozzi's high responder mouse strain in the generation of antibody secreting hybridomas.

While preparing monoclonal antibodies against human lymphocyte surface antigens, we compared two strains of mice, which had been genetically selected by Biozzi et al. (1970) for their high and low antibody responses to sheep erythrocytes. Biozzi et al. (1975) subsequently showed that these high and low responder strains (HR and LR) have a high and low antibody response to almost any antigen. We observed with the HR strain a huge increase in the number of hybrids secreting an antibody directed against human lymphocytes.

Animals↗
PubMed1980

The biological meaning of prostaglandin-levels.

Two groups of term pregnant rats, one pretreated with progesterone (P) and another with vehicle only, were induced to deliver with PGF2 alpha. Through an implanted intrauterine (extraovular) catheter 5 microgram PGF2 alpha was injected into each animal every 15 minutes until delivery occurred. If induction fialed the fetuses were removed by hysterotomy. All vehicle controls started delivery within 38.3 +/- 11.7 minutes. In contrast, none of the P-treated rats responded to PGF2 alpha. In all animals uterine vein blood and uterine tissue were collected during labor. Measurements of the P and PGF in uterine vein plasma and uterine tissue showed that the P-treated rats had significantly higher P-levels than the controls (P less than 0.001). In contrast, the PGF-levels were similar in the two groups, but significantly elevated (P less than 0.001) beyond spontaneous labor values. Evidently, the massive elevation of PGF-levels only induces labor when the myometrial action of P is critically reduced and is ineffective when P-action is sustained. Thus, PGF-concentrations cannot be presumed to predict PGF-effect, because the latter is controlled by P.

Animals↗
PubMed1980

Comparison, by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, of urinary proteins excreted by workers exposed to cadmium, mercury or lead.

Urinary proteins from workers exposed to cadmium (Cd, n = 20), lead (Pb, n = 19) or mercury (Hg, n = 25) were analyzed by polyacrylamide gel electrophoresis (PAGE) in the presence of sodium dodecyl sulfate (SDS). The areas of the peaks with the relative mobilities of beta 2-microglobulin and retinol binding protein (low molecular weight proteins), albumins, transferrin and IgG (high molecular weight proteins) were measured and compared with those obtained in matched control groups. The concentrations (mean and range) of the metals in blood (B, micrograms/100 ml) and in urine (U, micrograms/g creatinine) of the exposed groups were as follows: Cd group, CD-B = 1.51 (0.18-4.31) and CD-U = 16.6 (2.08-59.3); Pb group, Pb-B = 44.5 (44.8-61.3) and Pb-U = 88.7 (15.2-298); Hg group, Hg-B = 3.74 (1.84-8.14) and Hg-U = 116 (51.8-206). The average duration of exposure to the metal was 30.5 (Cd), 12.5 (Pb) and 7.2 (Hg) years respectively. Under these exposure conditions, it was found that Cd increases the urinary excretion of low as well as high molecular weight proteins, Hg induces an increased urinary excretion of only high molecular weight proteins and lead has no significant effect on the renal excretion of either type of protein.

Cadmium↗
PubMed1980

[Puromycin action on intestinal lipid absorption in male and female rats].

The effect of puromycin on absorption balance and mucosal lipid distribution was studied during intestinal absorption of 14C oleic acid and 3H palmitic acid, using the isolated intestinal loop of male and female rats in situ. In puromycin-treated animals, mucosal lipid uptake was reduced and mucosal lipid content increased, particularly with oleic acid and in male rats. The phospholipids were significantly reduced. As revealed by mucosal accumulation of free fatty acids, esterification was particularly inhibited with oleic acid and in female rats. These results might explain why portal blood radioactivity was higher in treated rats than in the controls. Puromycin effect occurred at different stages of lipid absorption and varied with sex.

Animals↗