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Biomedical subjects

A Bergenholtz

Publications and source records attributed to A Bergenholtz.

At least 37 records · Page 2Linked to original sources
PubMed1980

Flexor tendon specimens in organ cultures.

The healing process of sectioned and subsequently sutured rabbit tendon segments was studied over a period of 3 weeks, using an organ culture technique. In one series, the tendon specimens were exposed to a chemically defined culture medium for nutrition. In two control series, the specimens were kept in the synovial cavity of the knee joint for varying periods of time, before being transferred to the culture medium. The tendons remained viable in the medium. The superficial tendon cells demonstrated the morphological characteristics of fibroblasts, but cellular fibroplasia could not be detected. The two control series subjected to synovia prior to transfer into the culture medium showed superficial repair similar to the findings in previous studies on healing capacity of tendon nourished by synovia. The investigation supports the hypothesis that superficial tendon cells are fibroblasts with a potential for repair and that synovia is an efficient nutrient medium. Thus, the beneficial effects on repair exercised by the tendon sheath function should be utilized in flexor tendon surgery.

Animals↗
PubMed1977

Scanning electron microscopy of palatal mucosa maintained in organ culture: a method for three-dimensional visualization of cell morphology.

A scanning electron microscopic (SEM) study of palatal mucosa maintained in organ culture (50% O2-Eagle's MEM w/o serum) from 12 to 360 hours is presented. The morphological changes occurring in the explants during culture are illustrated and the findings are compared with those observed using transmission electron microscopy and light microscopy. The results confirm that cellular fine structure in epithelium and connective tissue is more easily visualized when SEM is employed following in vitro maintenance of explants as opposed to examination of fresh uncultured biopsies. The in vitro system used would appear to be applicable to studies designed to investigate the effect of various substances on cell proliferation and cell interactions.

Animals↗
PubMed1977

Oral, pharyngeal and laryngeal manifestations in Urbach-Wiethe disease.

Twenty-eight patients suffering from Urbach-Wiethe disease (UWD) and originating in northern Sweden are presented. The disease is characterized by amorphous deposits in the mucous membranes of the mouth, pharynx and larynx. Hoarseness since early childhood is the most common and striking symptom. Most of the patients also had severe changes in the true vocal cords. Characteristic deposits are also seen in the lower lip, tongue, palate and the posterior pharyngeal wall. These changes could be defined as pathognomonic of UWD. To confirm the diagnosis of UWD, biopsies should be taken from the skin or mucous membranes of the mouth. Biopsies from the vocal cords are not necessary and should be avoided.

Adult↗
PubMed1975

Oral manifestations in Urbach--Wiethe disease (lipoglycoproteinosis; lipoid proteinosis; hyalinosis cutis et mucosae).

The oral manifestations in 27 patients with Urbach--Wiethe disease (UWD) discovered in Northern Sweden are described. The oral regions most frequently affected are the lips, the back of the tongue, the frenulum of the tongue, the palate and the back wall of the pharynx. The general impression is that older patients usually have more marked manifestations than younger, indicating that the oral lesions may become more severe with increasing age. Histopathologically, the disorder is essentially a microangiopathy in which the walls of small blood vessels are thick and PAS-positive, indicating the presence of glycoproteins. In clinically affected regions there are usually PAS-positive extravascular deposits. In material used of lipid histochemical studies, sudanophil droplets were found in the vessel walls. By staining with osmium tetroxide the osmium is--contrary to previous assumptions--in some way bound to the droplets, but for unknown reasons is not reduced to a coloured product. The binding of osmium was demonstrated by the OTAN (osmium textroxide alpha-naphthylamine) method. The exact significance of this finding awaits further studies. The implications of dental anomalies occurring in UWD are discussed.

Adolescent↗