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Biomedical subjects

A Bergendorff

Publications and source records attributed to A Bergendorff.

7 recordsLinked to original sources
PubMed2000

Toxicological programmes and teaching methods.

Toxicological knowledge is required for three main purposes: basic and applied research, toxicity testing, and risk assessment. Curricula and teaching methods designed to meet these demands for knowledge are aimed at appropriation of relevant facts, appreciation of causality and an overall understanding of biological events caused by chemical substances. The Toxicology Programme at Karolinska Institutet is considered as an example for the design of appropriate courses. Teaching methods developed towards more self-tuition are becoming a natural part of such education. Continued escalation in scientific achievements and demands for safe use of chemicals will govern future trends in toxicological education.

Curriculum↗
PubMed1975

Intracellular distribution of amines taken up by rat mast cells.

Mast cells isolated from rat peritoneal and pleural cavities were incubated in vitro with radioactively-labelled histamine (Hi), 5-hydroxytryptamine (5-HT), dopamine (DA), noradrenaline (NA), tyramine (TA), phenylethylamine (PhEA), trptamine (TrpA), ephedrine (Eph) or amphetamine (Amph). All these amines were taken up by the mast cells. The dose-response curves for the compound 48/80-induced release of endogenous Hi and for the various amines taken up by the cells were compared. The release curves for Hi, 5-HT, DA, NA and TA were found to be similar to that for endogenous Hi, while those for PhEA, TrpA, Eph and Amph were different from that for endogenous Hi. The uptake of Hi, 5-HT, DA, PhEA, TrpA and Eph into granules in mast cells was studied. Membrane-bound granules were obtained by sonication of mast cells incubated with the respective amine, followed by differential contrifugation. The amine content of these granules was then measured. Hi, 5-HT and DA were found to be mainly localized to the granules, while a smaller proportion of the PhEA, TrpA and Eph was found there, the rest being located extragranularly. The present results suggest that, when taken up by rat mast cells, even amines which are not endogenous to the cells are stored in the same way as the endogenous amines Hi and 5-HT.

Amines↗