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Biomedical subjects

A Bengtsson

Publications and source records attributed to A Bengtsson.

At least 163 records · Page 9Linked to original sources
PubMed1985

DNA content in carcinoma of the rectum and rectosigmoid.

Using single cell cytometry the pattern of DNA content in preoperative samples from 131 carcinomas of the rectum and rectosigmoid has been studied. For comparison, 21 carcinomas of the colon, 44 colorectal polyps and mucosa adjacent to the tumours were also included. The biopsies were classified into near diploid (ND), near diploid with aneuploid cells (ND-anc) and aneuploid (AN). A more specific subclassification of aneuploid samples was not consistent because of a commonly occurring multiclonality, where different clones dominated different parts of the tumours. Fifteen of all 130 representatively sampled rectal carcinomas were ND; however, all the ND scores were based on a single sample. Among 31 multiply-sampled carcinomas none registered as ND in all samples. There were no principal differences in patterns of DNA profiles between rectal and colon carcinomas. Five samples from the pericancerous mucosa and samples from 4 adenomas showed non-ND patterns, which may be a premalignant sign. The main conclusions of these results are that rectal carcinomas, even if they contain a substantial ND cell population, to a very great extent contains cells with an abnormal DNA content. The carcinomas can be classified according to their pre-operatively estimated DNA content. The clinical relevance of this will be judged when the patients have been followed for longer time periods.

Adenocarcinoma↗
PubMed1985

Characterization of allergens and patient sera by a nitrocellulose immunoprint technique.

A rapid and convenient protein-gel blot technique for qualitative detection of antigens/allergens in pollen allergen extracts and IgE/IgG antibodies in patient sera has been developed. The antigens were separated by isoelectric focusing in agarose gel and transferred to nitrocellulose by capillary migration. After incubation of the nitrocellulose strips with serum from allergic patients, the binding of the patient's specific IgE or IgG antibodies was analyzed by using isotope-labelled or enzyme-labelled anti-IgE or anti-IgG. The time needed for detection with isotope-labelled antibody was approximately 20 h and with enzyme-labelled antibody 2 h. The immunoprint technique is easy to use, which renders it suitable for routine use in allergy research and quality control.

Allergens↗
PubMed1985

Lymphocyte stimulation in Reiter's syndrome. A comparative study between patients and normal controls with special reference to HLA-B27.

The lymphocyte-stimulating capacity of bacterial antigens from Yersinia enterocolitica and Chlamydia trachomatis was studied in 14 patients with Reiter's syndrome and compared with 16 healthy controls. The influence of HLA-B27 was evaluated. There was an increased reactivity in patients compared with controls. The most pronounced reactivity was seen in HLA-B27-positive patients. These findings support the hypothesis that there is an impaired T-cell function in HLA-B27-associated diseases.

Adult↗
PubMed1985

Immunological interference of high dose corticosteroids.

High-dose corticosteroids (HDC) will influence cellular as well as humoral participants of the immune response. The lymphoid tissue will decrease in size and weight after prolonged treatment with HDC. Lymphocyte functions will be impaired. Reduced synthesis of B- as well as T-lymphocytes will be seen. The inhibitory effect on B-cell function can be observed both as decreased serum levels of immunoglobulins and as impaired binding of antibodies and complement to the cellular surface. Reduced T-cell function indicated by impaired stimulation by PHA and porkweed as well as by impaired lymphokinin effects on leukocyte migration inhibition has been reported. Reduced lymphocyte adherence to antigen and suppressed lymphocyte reaction have also been observed. Humoral factors involved in chemotaxis, opsonisation, phagocytosis, vascular permeability leading to leakage of fluid and cells and factors involved in lysis of antigens are impaired. This can be explained partly by the observed reduced complement activation via the alternative as well as the classical pathway in association with HDC therapy. Acute processes with increased vascular permeability and accumulation of leukocytes as impairing factors could be influenced beneficially by HDC therapy. This positive effect can be seen in treatment of septic shock or rejection of a transplant. However, if sepsis or rejection is not rapidly reversed, complications such as multisystem organ failure and bacteremia are prone to appear.

Adrenal Cortex Hormones↗
PubMed1983

An unnecessary case of paraffin oil pneumonia.

A 64-year-old woman died after 12 years of progressive pulmonary disease which was initially diagnosed as sarcoidosis but later correctly identified as mineral oil pneumonia due to insufflation of paraffin instilled as nasal drops. In view of the potential damaging effect of liquid paraffin on the lungs, the current indications for its use must be strongly questioned.

Autopsy↗
PubMed1983

Intestinal permeability to different-sized polyethyleneglycols in patients with rheumatoid arthritis.

The intestinal permeability to polyethyleneglycols (PEGs) of varying size in patients with rheumatoid arthritis has been investigated. Permeability was determined by measuring the 6-hour urinary recovery of different-sized PEGs after oral intake of PEG 400, PEG 1000, or PEG 3000. Patients with rheumatoid arthritis excreted significantly less PEG 400 and PEG 1000 than healthy individuals, whereas the excretion of PEG 3000 was the same or even greater than in healthy individuals. These findings point to the possibility that intestinal permeability is altered in rheumatoid arthritis.

Absorption↗
PubMed1983

Bacteriological findings in 25 patients with Reiter's syndrome (reactive arthritis).

Extensive bacteriological testing was performed in 25 patients with complete or incomplete Reiter's syndrome. In 21 patients (84%), evidence of antecedent infection was found, based on culture and/or serological findings. Thus, infection with Chlamydia trachomatis (17 patients), Yersinia enterocolitica, serotype 3 (4 patients), and Campylobacter jejuni (1 patient) was identified.

Adult↗
PubMed1983

Reactive arthritis after campylobacter jejuni enteritis. A case report.

A 36-year-old HLA B 27 positive woman developed reactive type arthritis a week after enterocolitis. Stool culture and serological tests indicated Campylobacter jejuni infection. Serological evidence of previous Chlamydial infection was also obtained. The arthritis ran a benign course of 6 months, leaving no residual symptoms.

Adult↗
PubMed1983

Nuclear DNA in carcinoid tumours of the lung.

Fluorescence cytophotometric DNA measurements on 15 carcinoid tumours generally yielded histograms with a prominent G1-phase peak and only low frequencies of S- and G2-phase nuclei. In 13 tumours the modal DNA value was near diploid (1.75-2.25). Three of these 15 tumours showed a much greater number of S- and G2-phase values than the others and in two of them there were also G1-phase peaks below the near-diploid range. Among 22 samples of small cell carcinoma of the lung (SCCL), the modal DNA value was hypodiploid in two, near diploid in seven and hyperdiploid in 13. The proportion of S-phase cells was higher in the SCCL samples than in the carcinoid tumours. It is suggested that the nuclear DNA distribution profile may facilitate morphological discrimination of a classical pulmonary carcinoid from small cell carcinoma. In a borderline case, the finding of a hyperdiploid DNA stem line and high proliferative activity will favour the diagnosis of SCCL.

Adolescent↗
PubMed1982

Distribution of DNA between sister cells during serial subcultivation of human fibroblasts.

The segregation of DNA at the time of cell division was analysed by measuring the DNA contents of cells in mitosis and the incorporation of tritiated thymidine (3H-TdR) on each half of anaphases and telophases. Results suggest that the spreads in the 2C and 4C DNA contents are due to quantitative differences which could originate during semi-conservative DNA synthesis, chromosome assembly and chromosome segregation. This continuous rearrangement of the genome could lead either to a degenerative process or to a differentiation program.

Cell Cycle↗
PubMed1982

Diagnostic application of nuclear DNA measurements on bronchial secretions.

Bronchial washings from 254 patients with suspected pulmonary malignancy were investigated independently by two methods: bronchial cytologic examination and DNA measurements. For the latter, aneuploidy was used as a criterion of malignancy. At the time of the clinical follow-up, pulmonary malignancy had been verified by histopathologic examination in 91 of these 254 patients. For bronchial cytology the sensitivity was 40% and the specificity was 98%; for the DNA measurements the sensitivity was 37% and the specificity was 99%. Used in combination, the two methods showed a sensitivity of 46% and a specificity of 98%.

Adult↗
PubMed1981

Nuclear DNA measurements in squamous cell carcinoma of the lung: a guide for prognostic evaluation.

Cytophotometric analysis of nuclear DNA in 30 squamous cell carcinomas showed in most tumours a peak value near 2c (diploid) or 3 c (aneuploid), and a wide distribution of other nuclear DNA values. A follow-up study showed that the prognosis was best for patients with diploid tumour cells and worst for patients with aneuploid tumour cells. The DNA histogram patterns give information about the dominating DNA ploidy of the tumour cells; in some tumours information about the tumour proliferation activity is also provided. These data are useful in prognostic evaluation and may prove useful as adjuncts in the diagnosis and in selection of treatment.

Aged↗
PubMed1977

Nuclear DNA-content of parathyroid cells in adenomas, hyperplastic and normal glands.

Nuclear DNA-content in parathyroid cell imprints from adenomas, hyperplastic and normal glands was determined. Two principally different staining procedures were applied: paratoseaniline-Feulgen and ethidium-bromide staining. All nuclei, particularly with the ethidium-bromide method were registered as containing even multiples of the normal human diploid DNA-content. Normal and hyperplastic glands showed greater than or equal to 98 per cent diploid nuclei, the remaining fraction was tetraploid. In adenomas the frequency of diploid nuclei varied from 50-98 per cent; the remaining were predominantly tetraploid, but octaploid and higher even ploidity also occurred. A good correlation was found between nuclear DNA-content and nuclear diameter. The findings indicate that determination of nuclear DNA-content of parathyroid cells may be a valuable contribution in the differentiation between hyperplasia and adenoma.

Adenoma↗