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Biomedical subjects

A Benedetti

Publications and source records attributed to A Benedetti.

At least 325 records · Page 18Linked to original sources
PubMedDate not supplied

In vitro and in vivo effects of chloramine T on rat serum elastase inhibitor.

Recent studies have shown that oxidizing agents may block the elastase inhibitory activity of alpha 1-antiproteinase in humans and some animal species. It has been postulated that this protein plays a critical role in modulating the activity of the neutral proteinase, i.e. elastase, in the lung; its inactivation has been implicated in the destruction of lung tissue seen in emphysema. In this work, we have studied the inactivation in vitro of rat serum elastase inhibitor by chloramine T (CT) and whether the in vivo use of the same oxidizing agent in the development of a functional model of alpha 1-antiproteinase deficiency in the rat is feasible. Although serum alpha 1-antiproteinase is readily inactivated in vitro by CT, it was observed, in vivo, that serum elastase inhibitory capacity was reduced to about 28-35% of initial levels 1-3 h after CT injection, and returned to control values within 9 h. Therefore, the in vivo study demonstrated that in the rat a functional model of alpha 1-antiproteinase deficiency cannot be achieved by inactivation of the protein molecule with CT. The relatively short half-life (1.45 h) of the serum elastase inhibitor found in normal rats is consistent with a rapid synthesis of the protein molecule, which might contribute to the fast recovery of the elastase inhibitory capacity observed in experimental animals after CT administration.

Animals↗
PubMedDate not supplied

Stereotactic exploration of deep-seated or surgically unamenable intracranial space-occupying lesions.

Stereotactic biopsy of surgically unamenable intracranial lesions has been performed in our Neurosurgical Division since 1977. Riechert's apparatus is employed. An originally developed biopsy instrument and its advantages are described. Stereotactic coordinates of the biopsy site are found by CT scanner data using the technique described earlier by us. The series consists of 20 patients in whom a variable number of biopsy samples (from 1 to 5) was taken. Mortality and morbidity were nil. In 2 cases the stereotactic exploration led to a therapeutic maneuver: a cystic craniopharingioma and a cerebellar hematoma were extirpated. The clinical, neurological and histological features of 5 paradigmatic cases are illustrated. The relevance of histological diagnosis for adequate treatment is stressed. The indications for this surgical procedure are discussed.

Adult↗