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Biomedical subjects

A Banerji

Publications and source records attributed to A Banerji.

At least 37 records · Page 2Linked to original sources
PubMed1989

Fate of leucine in the biosynthesis of bakuchiol, a meroterpene from Psoralea corylifolia.

Significant incorporations of labelled leucine, valine and isovaleric acid into the meroterpene, bakuchiol (1) isolated from the medicinal plant, Psoralea corylifolia have been observed. Degradation experiments show that labels from these substrates find their way into both phenylpropane derived as well as terpenic part of 1 thereby indicating that none of the known pathways is operative in the case of 1. It is suggested that these substrates are metabolised to CO2 which is then incorporated into 1.

Fabaceae↗
PubMed1985

Incorporation of trans fatty acids into submandibular salivary gland lipids.

Three groups of rats were fed diets containing 20% corn oil, 20% margarine stock (MS) or 19% MS + 1% corn oil. Diets were fed for 12 weeks, 1 week of pregnancy, 3 weeks of lactation and 8 weeks post-weaning. The incorporation of trans-octadecenoate into various lipids of the submandibular salivary gland (SMSG) homogenates and plasma membranes was studied. Trans octadecenoate was incorporated into all the lipid fractions studied. Its levels were the highest in phosphatidylethanolamine. The double bond index of phospholipid fatty acids in the plasma membranes of the SMSG was substantially lower in the group fed 20% MS. The fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) was generally higher in the membranes of SMSG from rats fed MS than that of the other two groups, thus indicating lower fluidity. Also, the breakpoints in fluorescence polarization were at a higher temperature in the membranes from rats fed MS as compared with those fed corn oil. Lower fluidity of plasma membranes of SMSG observed in rats fed 20% MS may result in modification of the activities of membrane-bound enzymes.

Animals↗
PubMed1985

Membrane fluidity and adenylate cyclase activity in the lacrimal glands of rats fed diets containing trans fatty acids.

The effects of feeding a diet containing trans fatty acids on the fatty acid composition of plasma membrane phospholipids, fluorescence polarization of diphenylhexatriene (DPH) and adenylate cyclase activity in the exorbital lacrimal glands of rats were studied. Three groups of male, weanling rats were fed semipurified diets containing 20% corn oil (CO), 20% partially hydrogenated soybean oil (PHSBO) (a source of trans fatty acids) and 19% PHSBO + 1% CO. Plasma membranes of the lacrimal glands from rats fed 20% PHSBO showed higher adenylate cyclase activity and lower fluidity as shown by a lower double bond index of the fatty acids of their phospholipids and higher fluorescence polarization of DPH. When 1% CO was included with the diet containing PHSBO, the adenylate cyclase activity and membrane fluidity tended to be normal. The results suggest that feeding of a diet containing trans fatty acids in the absence of sufficient linoleic acid (18:2) can result in a decrease in membrane fluidity and an increase in adenylate cyclase activity in the lacrimal glands.

Adenylyl Cyclases↗
PubMed1985

Delta-9-Tetrahydrocannabinol: effect on mitochondrial swelling of different tissues of rat.

In vivo delta-9-tetrahydrocannabinol (delta-9-THC) under short (10 and 50 mg/kg) and long term (10 mg/kg/day for 15 consecutive days) administration (i.p.) to adult male albino rats increased the mitochondrial swelling of hypothalamus, heart and liver. Similarly, in vitro treatment with delta-9-THC (2-8 micrograms/mg protein) produced a dose-dependent stimulation in the mitochondrial swelling of different tissues. Ca2+ ions (0.15 mM - 0.3 mM) increased the mitochondrial swelling. Calcium (0.3 mM)-induced stimulation of mitochondrial swelling was enhanced with delta-9-THC under both in vivo and in vitro conditions. Chaotropic agents e.g. KNO3 (100-200 mM) and KSCN (200-400 mM), decreased the mitochondrial swelling. Chaotropic (200 mM)-induced inhibitory effect in different tissues was reduced with delta-9-THC under in vivo and in vitro treatments. These results suggest that delta-9-THC, like calcium, stimulated the mitochondrial swelling, and that the action of delta-9-THC may be localized on the specific structures of the membrane which are sensitive to chaotropic agents.

Animals↗
PubMed1982

In vivo autoregulation of rat adenohypophyseal thyrotropin-releasing hormone receptor.

The possible mechanism of attenuation of thyrotropin response to exogenous thyrotropin-releasing hormone in vivo after repeated administrations of the releasing hormone has been studied. To this end, the effect of prolonged hormone treatment on the binding of hormone to its receptor in the anterior pituitary gland has been evaluated. The data show that prolonged hormonal treatment resulted in a reduction in the number (B max) but no the binding affinity (KD) of the receptor. The effect was reversible and depended on the duration of treatment. This phenomenon of down regulation or the decrease in the receptor number was found not to be due to either the metabolism of releasing hormone or its ability to activate pituitary-thyroid-axis.

Animals↗
PubMed1982

Age-related changes in the in vitro glucocorticoid responsiveness of rat spleen and splenic leukocytes.

Specific in vitro glucocorticoid uptake and binding activity of spleen and splenic leukocytes were examined in adult (2- to 3-month-old) and senescent (24- to 28-month-old) male, adrenalectomized Sprague-Dawley and Wistar rats. No significant differences in these parameters were evident between young and old animals of either strain. Moreover, cortisol inhibition of [5-3H]-uridine uptake and the total number of glucocorticoid binding sites of spleen and its leukocytes were found to be similar in these two age groups. It was concluded that age has no effect on the glucocorticoid responsiveness of the splenic tissues of the two strains of male rats studied.

Age Factors↗
PubMed1982

The postnatal development of the pituitary thyrotropin-releasing hormone receptor in male and female rats.

The density of the pituitary TRH receptor in male and female rats was measured during postnatal development. The younger rats of both sexes exhibited higher [3H]TRH binding than did adults. A comparison of the properties of receptor binding between 4-5 day-old and adult pituitary showed that the increased binding was solely due to an increase in Bmax (maximum binding capacity) and not the KD (binding affinity). In conclusion, these findings demonstrate that the increased TRH-receptor density may be a contributing factor in eliciting exaggerated TSH response to exogenous TRH in neonatal rats.

Animals↗
PubMed1981

Development of an [3H] glucocorticoid exchange assay in rat liver cytosol.

An exchange assay for the assessment of glucocorticoid binding sites both free and steroid bound, in rat liver cytosol has been developed. The procedure, which is straight forward and noncumbersome involves the utilization of two sulfhydryl interacting agents, p-hydroxymercuribenzoate and dithiothreitol. The former in low concentration dissociates the steroid from the glucocorticoid receptor complex in relatively short time. The latter regenerates, quite rapidly and with good yield, the glucocorticoid binding activity of the receptor treated with the mercurial compound. The high recovery (99%) of the hormone binding activity of the receptor may be due to the few steps involved in the procedure. The exchange assay when applied to a physiological experimental situation was found to be valid and gave a true measure of total receptor content in rat hepatic cytosolic preparations.

Animals↗
PubMed1980

The metabolic fate of [35S]-diallyl disulphide in mice.

Diallyl disulphide (DADS) is a major constituent of garlic oil. Uptake of [35S]-labelled diallyl disulphide by mouse liver is highest at 90 min after treatment with [35S]-DADS, 70% of the radioactivity is present in the liver cytosol of which 80% is metabolized to sulphate.

Allyl Compounds↗
PubMed1980

Acetylene reduction assay for nitrogenase activity: gas chromatographic determination of ethylene per sample in less than one minute.

Ammoniacal silver nitrate (10 mg/ml) was added to terminate acetylene reduction assays used to measure nitrogenase activity. Silver nitrate quantitatively precipitated acetylene as the carbide salt, but did not affect the ethylene formed. The vials containing ethylene can be analyzed gas chromatographically at the rate of about 13 samples in 10 min.

Journal Article↗