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Biomedical subjects

A Balázs

Publications and source records attributed to A Balázs.

At least 19 recordsLinked to original sources
PubMed2005

Phenolic and triterpenoid antioxidants from Origanum majorana L. herb and extracts obtained with different solvents.

Antioxidant properties of marjoram (Origanum majorana L.) herb and extracts obtained with ethanol, n-hexane, and supercritical CO2 extraction are presented. Individual antioxidants, ursolic acid, carnosic acid, and carnosol, were quantified with high-performance liquid chromatography. The effects of different parameters (temperature and pressure) of high-pressure extraction on the yield of carnosol were studied. Furthermore, two marjoram herbs from Hungary and Egypt were compared measuring hydrogen-donating abilities with 1,1-diphenyl-2-picrylhydrazyl by spectrophotometric and the total scavenger capacities by chemiluminometric methods from the aqueous extracts of the herbs. The antioxidant activities of the solvent extracts were performed using the Rancimat method. The Egyptian herb and its extracts possessed better antioxidant activities than Hungarian ones. Applying supercritical CO2 extraction, the highest value of carnosol was obtained at 400 bar and 60 degrees C.

Antioxidants↗
PubMed2003

In vitro analysis of the properties of Beiqishen tea.

OBJECTIVE: Chinese Beiqishen tea was studied in an in vitro test system. METHODS: Phytochemical screening, trace element analysis, and the analysis of antioxidant properties were carried out. Characteristic constituents were determined by chromatographic (capillary gas chromatography and GCQ Ion Trap mass spectrometry) and spectrometric (ultraviolet and UV-VIS) methods. Element concentrations were determined by inductively coupled plasma optical emission spectrometry. Antioxidant capacity was studied by spectrophotometric and luminometric techniques using a Berthold Lumat 9501 luminometer. Hydrogen-donating activity, reducing power, and total scavenger capacity were measured. RESULTS: Total polyphenol content was 20.77 +/- 0.52 g/100 g of drug; total flavonoid content was 0.485 +/- 0.036 g/100 g of drug; and tannin content was 9.063 +/- 0.782 g/100 g of drug. Caffeine content was 1.08 mg/100 g of drug. Essential oils were identified by gas chromatography: (+)-limonene (21%), p-cymene (1.7%), estragol (3.2%), beta-ocimene (1.4%), and thymol (2.6%). Metallic ion analysis showed significantly high concentrations of Al, As, Ba, Cr, Cu, Fe, Mn, Ni, and Ti in the drug. Antioxidant and scavenger properties were identified as a function of concentration. CONCLUSIONS: The tea infusion contained some non-desirable trace elements and caffeine in addition to polyphenols and tannins in high concentrations. Therefore, the consumption of this tea may involve risks.

Alkaloids↗
PubMed2001

[New data on composition of esssential oil from inflorescence of everlasting (Helichrysum arenarium(L.) Moench.)].

We investigated the composition of essential oil obtained from inflorescence of Helichrysum arenarium (everlasting) which is a perennial herb, native in our home and in Middle and South East Europe. We used essential oils obtained from Hungarian and Polish mercantile samples and a plant sample cultivated in Hungary (Soroksár) by steam-distillation. The GC and GC-MS investigation revealed the presence of more than 60 compounds from which 24 were identified. The identified compounds are as follows: linalool, alpha-terpineol, carvone monoterpenes; anethol, anisaldehyde, thymol, carvacrol, eugenol, beta-asarone, butylhydroxyanisole aromatic components; alpha-humulene, beta-caryophyllene, gamma-muurolene, delta-cadinene, copaene, alpha-gurjunene, caryophyllenol, delta-cadinol and globulol sesquiterpenes as well as caprilic, pelargonic, caprinic, laurinic acids and methyl palmitate as alkyl carbonic acid and their derivatives. We established that the main components of essential oil of Hungarian and Polish mercantile samples is methyl palmitate (28.5% and 21.7% respectively) but of cultivated sample is caprinic acid (19.8%). Comparing our results of essential oil composition with those obtained from other Helichrysum species it is surprising that the alkyl carbonic acids are the dominant essential oil constitutents of Helichrysum arenarium.

Acyclic Monoterpenes↗
PubMed2001

Dominance relationships of bean pathogens at Lake Balaton.

Dominance relationships of different bean pathogens were examined during 1999-2000 in small plot trials at Lake Balaton in Hungary. In 1999 the dominant pathogen species were Xanthomonas campestris pv. phaseoli. The main cause of the stock decay was due to the infection of Fusarium spp. Bean plants were infected also by Alternaria, Colletotrichum, Macrophomina and Sclerotinia, species part from viruses. Among of thirty-eight examined bean cultivars and genotypes the variety "Díszbab" and the genotype 513 were the most resistant. In 2000 Macrophomina phaseolina and Fusarium spp. caused epidemics. Most of the observed plants died early. The most healthy species and branches were the SC-34-1 and cv. Díszbab.

Alternaria↗
PubMed2000

[A case of retroperitoneal pancreatic abscess spreading to the femoral region].

Authors report the case of a 47 years old male patient with acute femoral abscess. The examinations made with urgency found proceeding acute pancreatitis, fluid collection in the right pleural cavity, exceeding fluid collections in the retroperitoneum and right paracolic region. The inflammatory infiltrate and collection in the upper third of the right thing seemed to be in connection with the proceeding pancreatitis. Because of the process endangering also the viability of the limb and severe septic state, an acute operation was performed, in the course of which extension of the retroperitoneal abscess to the thing was observed. After abdominal oncotomy expanded to thigh and inguinal region, lavage, drainage operations, therapy with wide spectrum followed by aimed antibiotics general condition of the patient improved. On the 18 postoperative day a new feverish state manifested. Image forming examinations showed newer purulent collections in the abdominal cavity and on the thigh in addition to the previous abscesses. Because of this repeated exposure was necessary. After the second operation the patient recovered without complaint and further complications. Authors think the case worth attention because of the extensive and unusual localization of abscesses formed beside the relatively discrete abdominal complaints.

Abscess↗
PubMed1998

Development of ELISA and enzyme-linked immunofiltration assay (ELIFA) methods for monitoring cyclodextrin glycosyltransferase (CGTase) production and bacterial growth in Bacillus macerans batch cultures.

Immunochemical methods were developed for monitoring cyclodextrin (CD) glycosyltransferase (CGTase) production and growth of an industrial CD-producing Bacillus macerans strain. Extracellular concentrations of CGTase released into a non-transparent culture medium during a 44 h long fermentation were detected by an indirect antigen inhibition enzyme-linked immunosorbent assay (ELISA). The ELISA was sensitive (minimal detection level 6 ng ml-1) and highly reproducible (coefficients of variation < or = 1.2 and 5.9%, within-runs and between-runs, respectively) compared to assays of CGTase activity (coefficients of variation < or = 4.2 and 7.0%, respectively). The ELISA, in combination with enzyme activity measurements, was useful to detect the decrease in the specific CGTase activities after 36 h of incubation, which was clearly indicative of the proteolytic degradation of CGTase. B. macerans cell numbers were estimated using an enzyme-linked immunofilter assay (ELIFA). The assay took less than 1 h and the coefficients of variation within and between-runs (2.9-6.4%) were considerably less than for viable counting (10.6-15.4%). In the exponential phase of growth, ELIFA results correlated more closely with the cell counting based on total protein than with viable counts. Nevertheless, in the phase of cell lysis, the bacterial cell number was systematically underestimated by ELIFA in comparison to both viable cell number and total protein determinations. Thus cell antigens detected with immunological procedures might be lost during the transition from vegetative cells to spores. On the other hand, the ELIFA procedure was specific for B. macerans cells and was a better indicator of the onset of the different growth phases than the cell numbers calculated from the protein assay.

Bacillus↗
PubMed1997

[A case of unusual complication of diaphragmatic herniation of transverse colon following transhiatal esophagectomy].

The authors report a rare complication observed after transhiatal esophagus extirpation performed for esophageal cancer. In this case the transverse colon herniated into the pleural cavity through the esophageal hiatus. Herniation completed on the 6.th. postoperative day and caused mechanical ileus. In the first few postoperative days, radiology pointed to a basal pulmonal infiltrate, later it resembled relaxation of the diaphragm, which was rather misleading. Recognition of the real situation was possible only on the 6.th.postoperative day. The patient was reoperated and the pathological state could be reversed. The esophageal hiatus was reconstructed. In the opinion of the authors the complication may have developed partly due to the opening of the left pleural cavity in the course of extrathoracal esophagectomy, and partly to the fact that the spleen was removed during operation. The outcome after reoperation was uneventful. The authors consider this as a rather rare and instructive case.

Colon↗
PubMed1991

[Current concept of autism].

Childhood autism, the most characteristic subgroup of the broader PDD (pervasive developmental disorders) category, is the consequence of genetic or typically prenatal, organic factors causing cerebral damage. The resulting mental handicap lasts for a lifetime. It is characterized by a behavioural syndrome, that becomes recognizable between the 2.-3. year. The core of the syndrome is a deviant and/or retarded development of cognitive capacities and skills necessary for social relations, communication, fantasy and symbolic thinking. Almost all autistic people (95%) would not reach independence as adults, and most of them (75%) is mentally retarded as well. According to our calculations about 16,000 people are affected in Hungary, in a more or less serious degree, 2000 children among them. Their condition would require intensive, early and long lasting intervention: conditioning, training, education, and special social services. Today we do not possess the necessary organisational background, nor the professional training, and knowledge. For the early diagnosis, proper care and services the competence of child- and general psychiatrists, also of family doctors is indispensable. The author summarizes the most important available informations on the field first of all for those, who work in the general medical services.

Autistic Disorder↗
PubMed1991

The effect of the conformer state and the model size chosen on the force field of the polypeptide backbone.

We have attempted to check the validity of our previously calculated ab initio force field for the polypeptide backbone, for the variance with conformer state and model size chosen. For the previous problem we applied our usual ab initio Hartree-Fock SQM method, also used at the original development of the force field, while for the latter we utilized the semiempirical MNDO CO-gradient method. It is shown that the in-plane force field is reasonably stable for change of conformational state, while the out-of-plane force field can be assigned good average guess values. The effect of the model size is shown to be moderate upon introducing a dipeptide into a polymer chain.

Alanine↗
PubMed1991

[Endoscopic intubation and intracavitary irradiation in the palliative treatment of inoperable esophageal tumors].

The authors have applied at first in Hungary the intracavital after loading radiotherapy in patients suffering from esophageal tumor. A new method was elaborated for the combined use of endoscopic tube insertion and intracavital irradiation. Out of the 155 patients admitted on the 1st Surgical Department of Semmelweis University, Budapest, 63 had been treated only by tube insertion, 44 were intubated and irradiated, whereas 48 patients had intracavital radiotherapy without intubation. Radiotherapy was performed at the Radiological Unit of Semmelweis University. The irradiation dose ranged from 26,9 to 32,2 Gray in average. The mean survival of group I. was 4,0 months, that of group II. 6,3 months, and in group III. 7,0 months respectively. The expected survival is proportional to the radiation dose. No correlation exists between tumor localisation and survival. Respiratory fistula, the most common complication, occurred in different groups as follows: 3,4%, 6,2%, and 9,0%; but could have been treated by tube insertion, or adequate positioning of the tube. The combined use of intubation and intracavital after loading radiotherapy has been proven to be suitable for palliative treatment of inoperable esophageal tumors.

Aged↗
PubMed1986

Susceptibility of the human promyelocytic cell line HL-60 to an endogenous antileukaemic factor in suspension cultures.

HL-60 human leukaemic cell line a suitable homogeneous target population for the selective endogenous inhibitor of myelopoiesis, isolated in our laboratory, was submitted to multiparameter analysis of cell proliferation in suspension cultures. As detected by 3H-TdR incorporation, a single dose of the regulator elicited a 6 to 8 hours arrest of DNA synthesis. The inhibition could be prolonged by repeated applications. As affected by the factor, alteration of population kinetics is characterized, revealed by flow cytofluorometric analysis, in G1 arrest of a fraction of cells, and diminishing those in hyperdiploid and tetraploid stage. 51Cr-release detection of vitality proved, that the endogenous factor, chemically determined as nucleopeptide, affected non-toxically and reversibly HL-60 cell proliferation.

Cell Line↗
PubMed1986

[Effect of a leukocytic serum preparation on hematopoietic cells in vitro].

The effects of leucocyte serum (LS) on bone marrow cells (BMC), thymus and HL-60 human myeloid leukemia cells were studied in liquid suspension and agar cultures. LS increased 3H-thymidine incorporation in BMC and intensified the cloning efficiency of granulocyte-macrophage progenitor cells (CFU-GM) and human myeloid leukemia cells. No significant stimulatory effect on thymus cells was observed. It has been shown that LS prevents or markedly decreases the effect of granulocyte inhibitor (GI-3S2).

Animals↗
PubMed1983

Synergism of endogenous inhibitors, exogenous cytostatic agents and hormones in arrest of proliferation.

The synergism between endogenous regulators of proliferation (protors), alkylating agents and hormones in vitro was studied. The effects were monitored by the incorporation of 3H-TdR into human and rat short term bone marrow cultures and by the formation of mouse granulocyte-macrophage colonies in semisolid agar capillaries. An additive and/or slight potentiating synergism was demonstrated between different types of inhibitory protors (GI-3S2, GI-3S3 and GI-3B), between GI-3 and hydrocortisone, and between GI-3 and the alkylating agents (adriamycin, dianhydrogalactitol) examined. The results offer a real possibility of strengthening the inhibition of neoplastic proliferation without increasing cytotoxicity of the drugs used.

Animals↗
PubMed1983

In vitro proliferation of normal and leukaemic human leukocytes controlled by an inhibitory endopeptide.

GI-3, an endogenous inhibitory fraction isolated from leukocytes, selectively inhibits the proliferation of granuloid precursor cells in a non-toxic manner. Its active principle was determined as an acidic chlor-tolidine positive decapeptide [ 3 ]. The in vitro effect on normal and acute leukaemic human bone marrow and blood cells was examined. A dose dependent inhibition by GI-3 of 3H-TdR incorporation into myeloid cells of normal bone marrow was found, the sensitivity of human cells being higher than that of rat cells. The proliferation of the target leukaemic bone marrow and blood cells (AML, AMMoL) was also decreased by the endogenous inhibitor in a dose dependent manner in untreated subjects as well as in patients in remission or relapse. The rate of inhibition of leukaemic of well-known cytostatics (adriamycin hydrochloride, dianhydrogalactitol) applied for comparison. Beyond its direct cytostatic effect, GI-3 could be used in the differential diagnosis of blastic leukaemias, complementing the routine cytochemical methods.

Adult↗
PubMed1981

Protors--an integrated theory on the regulation of proliferation, oncogenesis, and aging.

Growth factors, chalones, mitogen and mitodepressive hormones, retine, promine and other compounds, controlling cellular multiplication are investigated separately at different fields of biomedicine. To promote their mutual research, integration and common nomenclature protor is suggested as collective term for the different regulators of proliferation. The coherent comprehension of protors offers a possibility to unified explanation of cell proliferation, oncogenesis, and ageing. Carcinogenesis takes place by lack of inhibitory, or overproduction of stimulatory protors, caused by somatic mutation of regulatory genes. Ageing is characterized or may be influenced by the altered concentration of protors in static, expanding and renewing populations.

Aging↗
PubMed1980

Purification of an endopeptide to homogenity and the verification of its selective inhibitory action on myeloid cell proliferation.

The purification of an endogenous regulatory peptide to homogeneity which selectively inhibits the proliferation of normal and leukaemic myeloid cells is described. Leucocytes isolated from calf spleen or horse blood were homogenized, extracted by acetone, chloroform and distilled water, ultrafiltrated by Amicon Diaflo XM 50 and PM 10 membranes. The lyophilized filtrate was chromatographed on Sephadex G-15 and G-10 columns. Fractions were tested for the selective inhibition of myeloid proliferation by 3H-TdR incorporation and capillary colony formation. The active fractions were submitted to paper electrophoresis at pH 6.5 and 1.9, and the peptides were re-tested. Finally a ninhydrin negative and chlor-tolidine positive oligopeptide was identified as granulocyte-specific inhibitor, effective at 0.2 to 3.0 pmol/ml MED value in vitro. The peptide is negatively charged at pH 6.5, its electrophoretic mobility as compared to aspartic acid is -0.60. The peptide has no charge at pH 1.9, the mobility related to epsilon-DNP lysine is 0.26. Details of the structure analysis of the inhibitory endopeptide is described in our next paper.

Animals↗
PubMed1980

Preparation of a target specific fraction controlling the proliferation of granulocytes. The abolishment of the specificity by glutathion.

A low molecular weight fraction (GI-3) was prepared from peripheral horse blood leukocytes. This fraction inhibits the proliferation of bone marrow cells, the effect is dose dependent. The proliferation of thymocytes and HeLa cells is not affected by GI-3. This specificity was abolished when glutathion was applied together with GI-3; the 3H-TdR uptake of thymocytes in vitro was completely inhibited, however, slight or no effect was observed on bone marrow and HeLa cells, respectively.

Animals↗