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Biomedical subjects

A Bakke

Publications and source records attributed to A Bakke.

64 records · Page 4Linked to original sources
PubMed1981

Effects of hemorrhagic shock on gastric blood flow and acid secretion in cats.

Anesthetized cats were cannulated and successively bled to arterial pressures of 100, 75 and 50 mmHg. The pressure was maintained at each level for 20 min. One group of animals was given 16 mu . kg-1 . h-1 of pentagastrin and another group 2 mg . kg-1 . h-1 of cimetidine throughout the experiment. Blood flow was determined by means of the microsphere distribution technique using spheres 14 +/- 1 micrometer in diameter. At each pressure level 15 min sample of gastric juice were collected. Twenty ml of saline was instilled into the stomach and withdrawn together with the gastric juice. Adenosine triphosphate (ATP) content and enzymatic activity (LD, ASAT) of the gastric mucosa and blood pH, pCO2 were determined at 140 and at 50 mmHg. In both pentagastrin and cimetidine treated cats gastric mucosal blood flow decreased and the vascular resistance increased markedly when the pressure was lowered by bleeding from 140 to 100 mmHg. On further bleeding to 50 mmHg only slight additional flow reduction occurred and the vascular resistance remained constant. Under shock blood flow was higher and the resistance lower in pentagastrin than in cimetidine treated animals. In pentagastrin treated animals a parallel decrease in acid secretion and mucosal blood flow occurred under shock. Cimetidine inhibited acid secretion both at normal blood pressure and under shock. The ATP content of the mucosa did not decrease, no morphological changes of the mucosa occurred and there was no leakage of cellular enzymes during the period of hypotension.

Adenosine Triphosphate↗
PubMed1979

Identification of a cell surface glycoprotein involved in cell aggregation in D. discoideum.

Analysis of the composition of cell surface-associated glycoproteins of D. discoideum by lactoper-oxidase-catalyzed radioiodination, followed by isolation by Con A-Sepharose chromatography, revealed that the developmentally regulated cell surface expression of a certain glycoprotein (gp150) parallels the onset of mutual cellular cohesiveness (Geltosky, Siu and Lerner, 1976). We have purified gp150 and raised specific antibodies to it. Through utilization of the specific antibody and a fluorescence-activated cell sorter, the expression of gp150 on the cell surface has been studied. Starting from a low level in noncohesive (vegetative) cells, there is a rapid accumulation of gp150 on the surfaces of aggregating cells. A peak level of expression is achieved by 10 hr and maintained at least until the steps of terminal differentiation. Most significantly, monovalent Fa'b derived from anti-gp150, when added to aggregation-competent cells, blocks the cells' ability to reaggregate. Fab's derived from antisera with different specificities were ineffective inhibitors of cell aggregation. These results suggest that gp150 serves an intimate role in cell adhesion.

Antigen-Antibody Reactions↗
PubMedDate not supplied

Relationship of the 1 alpha, 25-dihydroxyvitamin D3 receptor concentration and the DNA content in renal cell carcinomas.

A role has been suggested for 1 alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3) in cell proliferation and differentiation. Therefore the concentration of the 1,25-(OH)2D3 receptors and DNA-ploidy was measured in 22 renal cell carcinomas. No relation was found, the mean 1,25-(OH)2D3 receptor concentration being 8.4 fmol/mg of protein (range 2.8-15.9) in diploid tumors and 7.0 fmol/mg of protein (range 0-27.8) in DNA aneuploid tumors. The aneuploid tumors (11 out of 22) had a heterogeneous DNA content in 9 out of 11 cases. At the time of operation, no patient had metastases. In this prospective study, one out of 9 patients with DNA aneuploid tumor had died of renal cell carcinoma (observed 33-58 months, median 42 months). None of 10 patients with diploid tumors had died (observed 40-56 months, median 52 months).

Aneuploidy↗