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Biomedical subjects

A Bagrel

Publications and source records attributed to A Bagrel.

11 recordsLinked to original sources
PubMed1981

Influence of oral contraceptives of differing dosages on alpha-1-antitrypsin, gamma-glutamyltransferase and alkaline phosphatase.

We have studied the effects of oral contraceptives on three glycoproteins, alpha-1-antitrypsin (A1AT), gamma-glutamyltransferase (GGT) and alkaline phosphatase (AP), in terms of age (20--40 years), duration of administration, and levels of estrogens. We have confirmed that oral contraceptives increase the concentration of A1AT and the activity of GGT and decrease the activity of AP, but that the modifications are less pronounced with pills containing low levels of estrogens for GGT and AP. We observed a decrease in A1AT and GGT after 2 and 5 years of treatment, respectively, but an increase in AP. Patient age seemed to have little influence on the change in AP activity and A1AT concentration but GGT activity was higher for the 25--30 year group.

Adult↗
PubMed1981

[Study of factors of biological variations acting on the concentration of the alpha-1-serous antitrypsin in a "common population" (author's transl)].

We established by nephelometry the serous concentration in an antitrypsin alpha-1 from an example of common population of th preventive Medicine Center of VAndoeuvre-lès-Nancy (675 adults men and women). First of all, the technical qualities of the equipment (replication : 0.06) to 1.1 per cent) and the dosage method (replication : 1,6 to 2,9 per cent, reproduction 3,4 to 4,3 per cent) were estimated. We showed that the technique was well connected with the electroimmunodiffusion (r : 0.90), but that was an important dissociation between the concentrations established by the two methods. The analytical variation being known, we approached the study of biological variations. The distribution of antitrypsin alpha-1 values is quite log-normal (median : 1,71 g/l, medium : 1,76 G/l). The main factors of biological variation observed are the sex (increase in 6 to 11 per cent in women), the age (decrease in 10 per cent between 20-30 years old and 40-50 years old women), the tobacco (increase in 9,5 per cent in the men smoking more than 20 cigarettes a day), the drinking of alcohol (decrease in 10 per cent for two sexes), the taking of oral contraceptives (increase in 29 per cent). These factors of biological variation should be taken in account at the time of producing the reference values, in addition of the analytical, pathological and genetic factors.

Adult↗
PubMed1980

Study of an enzyme immunoassay kit for carcinoembryonic antigen.

We evaluated a commercial enzyme immunoassay kit for carcinoembryonic antigen (CEA) based on a non-competitive "sandwich principle" method (Abbott Laboratories). The serum sample is treated with an acid buffer and the supernate is incubated with an anti-CEA coated polystyrene bead. After washing, the bead is incubated with an anti-CEA/peroxidase conjugate. After a second washing, the activity of the enzyme bound to the solid phase is assayed after addition of a chromogenic substrate. This activity is proportional to the concentration of CEA in the serum sample. The characteristics of the assay are: (a) good sensitivity (around 0.25 microgram/L) and (b) satisfactory reproducibility (CV < 11% within assay). There is little cross reactivity between CEA and molecules such as nonspecific cross-reacting antigens that are known for their high potential of cross reactivity. No nonspecific interference was encountered with anti-globulin factors. We compared results with this enzyme immunoassay kit with those by a radioimmunoassay provided by the Commissariat à l'Energie Atomique. The correlation (r) was 0.95 (p < 0.001). The distribution of CEA values obtained for 1020 normal subjects is given. We conclude that the kit provides a simple and reliable procedure.

Carcinoembryonic Antigen↗
PubMed1980

A study of factors influencing plasma CEA levels in an unselected population.

Plasma carcinoembryonic antigen (CEA) levels were measured by an immunoenzymic method (Abbott) in 1020 subjects attending the Preventive Medicine Centre (Vandoeuvre-les-Nancy). The results are assessed in relation to: sex, age, body build, fasting/normal food intake, smoking, alcohol intake, drug medication, and working environment. The mean plasma CEA level is 1.53 ng/ml. 87% of the total group has levels less than 2.5 ng/ml, 11.2% levels between 2.5 ng/ml and 5 ng/ml and 1.8% levels above 5 ng/ml. One person had a level above 10 ng/ml. Men had significantly higher CEA levels than women. Smoking was more frequent in both men and women with CEA levels above 2.5 ng/ml. Only in men were age, alcohol consumption and a poor work environment significantly associated with CEA levels higher than 2.5 ng/ml. Obesity in women was related to higher CEA levels. Food intake and drug medication were without influence on the CEA level.

Adolescent↗
PubMed1979

Relations between reported alcohol consumption and certain biological variables in an "unselected" population.

We looked for variables that could serve as indexes of alcohol consumption. We tested iron, gamma-glutamyltransferase, mean erythrocyte volume, mean erythrocyte hemoglobin concentration, urea, uric acid, and alanine aminotransferase, among others, in an unselected population of men, and by principal components analysis we singled out the correlation between the two enzymes and the two hematologic values. On the other hand, calculation of the coefficients of correlation between the total amount of alcohol consumed and the 10 variables studied, together with multiple regression analysis, showed that the three variables that correlated most significantly with alcohol consumption were gamma-glutamyltransferase, mean erythrocyte volume, and the use of tobacco. Critical evaluation of the results leads us to conclude that still more discriminative biological indices msut be sought and that the use of psychosocial data is also desirable.

Adult↗
PubMed1978

[Enzyme multiplied immunoassay technic (EMIT) (author's transl)].

In 1960, Yalow and Berson opened the route to radioimmunoassay. Now the technic of radioimmunoassay requires apart from the use of artificial radioisotopes, a compulsory stage of separation of free and linked phases. The author has developed a technic in homogeneous phase "EMIT" which avoids the intermediate stage of separation. He gives here the principle, studies the various parameters of the method, shows the advantages and disadvantages and compares it with other technics and finally mentions future prospects.

Antigen-Antibody Reactions↗
PubMed1976

Ornithine carbamoyl transferase activity. Use of a bovine liver extract in quality control.

To obtain a pure ornithine carbamoyltransferase for quality control in clinical chemistry, the authors compared human plasma and bovine liver ornithine carbamoyltransferase. The data show that the KM of both enzymes are similar (respectively 1.03 and 1.33 mmol when ornithine is used as substrate; 1.02 and 1.06 mmol with carbamyl phosphate). The following properties are shown to be equivalent: linearity of the enzymatic reaction, inhibition by higher substrate concentrations, and temperature denaturation. The similarities between the two enzymes indicate that bovine liver ornithine carbamoyltransferase can be used in clinical chemistry quality control.

Animals↗
PubMed1975

Colorimetric measurement of ornithine carbamoyl transferase activity in plasma, and results for a supposedly healthy population.

Determination of ornithine carbamoyl transferase (EC 2.1.3.3) activity in plasma is important for detection of liver diseases. The assay established in this paper has been made optimum. A blank is needed containing both substrates, carbamoyl phosphate and ornithine. We used a new colorimetric assay, based on a complex with a phosphoferric-antipyrine reagent and diacetyl monoxime, to measure the citrulline formed. The highly sensitive assay permits low activities to be determined accurately. Values for blood plasma from 425 supposedly healthy people, varied from 0 to 16 U/liter (95th percentile), and 27% of this population showed an activity of less than 2 mumol of citrulline formed per minute per liter, 2 U/liter being the limit of the method's sensitivity.

Colorimetry↗