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Biomedical subjects

A B Wysocki

Publications and source records attributed to A B Wysocki.

5 recordsLinked to original sources
PubMed1990

Fibronectin profiles in normal and chronic wound fluid.

Fibronectin, an adhesion protein found in blood and tissues, is an important factor in wound repair. Recently, we began studies using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting to compare fibronectin in the fluid of normal and chronic wounds. We found similar fibronectin profiles in suction blister fluid (2 normal volunteers) and mastectomy fluid (5 patients). There was primarily an intact 250 kilodalton fibronectin subunit and in addition some higher molecular mass fibronectin-containing complexes. On the other hand, fibronectin in diabetic ulcer wound fluid (2 patients) was partially degraded, and there was no intact fibronectin in stasis ulcer wound fluid (2 patients). The most prominent degradation products were 93 and 125 kilodalton polypeptides. When intact fibronectin was added back to stasis ulcer wound fluid, fragmentation occurred within 15 minutes at 37 degrees C indicating the presence of protease activity in the wound fluid.

Biopsy↗
PubMed1990

The healing wound: a comparison of three clinically useful methods of measurement.

A variety of different methods can be used to measure healing wounds. Among these are: 1) photographs, with subsequent area quantification using a digitizing pad with computer interface; 2) wound tracings; and 3) a Kundin measuring device. The purpose of this study was to compare three different clinically useful methods of measuring the area of healing wounds. Observations of venous stasis ulcers (n = 36) and decubitus ulcers (n = 37) were made on patients recruited from both inpatient and outpatient populations of a large metropolitan hospital. Analysis of the data using Pearson correlation revealed that all three methods were highly correlated, r = 0.93 or above (p less than or equal to 0.001). However, repeated measures analysis of variance among the three methods revealed that they were all significantly different (p less than or equal to 0.001) from each other. It was concluded that: 1) either of the three methods can be used as a valid index of wound area, but 2) acetate tracing should be used to obtain the most accurate measure of actual wound area.

Adult↗
PubMed1987

A microcomputer-based vision system for area measurement.

A vision system for measuring the area of an arbitrarily shaped object is described. The algorithm consists of a gray-level thresholding technique combined with a region correction procedure based on mathematical morphology. All processing steps are carried out on a microcomputer system equipped with a video digitizer. The algorithm has been successfully applied to a number of images of medical interest including skin wounds and various microscopic-scale objects such as cell cross-sections and multicellular tissues. Excellent agreement between results obtained by the automatic method and by using standard mechanical means has been established experimentally. The approach is demonstrated by a number of experimental examples.

Algorithms↗
PubMedDate not supplied

Fibronectin in acute and chronic wounds.

Fibronectin, a fibrillar glycoprotein, is the major adhesive protein deposited at the wound interface during acute wound healing. Its abilities to interact with both cells and matrix are responsible for its description and characterization as a multifunctional adhesive glycoprotein. Fibronectin functions to promote cell adhesion, cell shape and spreading, organization of the cytoskeleton, hemostasis and thrombosis, cell migration, and phagocytosis. Both in vitro and in vivo studies indicate that fibronectin interacts with cells important to wound healing, including platelets, neutrophils, monocytes, fibroblasts, endothelial cells, and epidermal cells. It promotes adhesion between cells and between cell and matrix, cell spreading, migration, chemotaxis, phagocytosis, and matrix and basement membrane organization. Fibronectin is consequently active during each stage of wound healing. Its importance in acute wound healing is now widely recognized. More recently, investigators have begun to study fibronectin's role in chronic wounds. This article briefly reviews fibronectin, outlines its role in wound healing, and discusses current research on fibronectin in chronic wounds.

Acute Disease↗