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Biomedical subjects

A B Tsypin

Publications and source records attributed to A B Tsypin.

At least 19 recordsLinked to original sources
PubMed1993

[Splenic perfusion in the combined treatment of patients with systemic lupus erythematosus].

Twenty patients suffering from severe systemic lupus erythematosus (SLE) underwent 52 sessions of splenoperfusion (SP) by means of extracorporeal perfusion of the patient's blood through isolated porcine spleen. It got prepared directly before the perfusion. The organ was obtained from a healthy animal in sterile conditions, washed from the blood after cannulation of the artery and vein. The patient's blood was pumped from the peripheral vein to the spleen artery. After passing through the splenic vascular bed the blood outflew from the spleen vein into the opposite peripheral vein of the patient. SP was followed by positive clinical changes: normalization of body temperature, regression of skin symptoms and articular syndrome, reduction of lymphadenopathy, hepato- and splenomegaly. The tests indicated inhibition of SLE activity. Sp-related immunocorrective effect was also present as shown by depression of B-cell hyperreactivity and T-cell immunity stimulation. 1-4-year follow-up of 19 patients showed them to be in remission. The data obtained favour SP inclusion into intensive therapy of severe SLE.

Acute Disease↗
PubMed1993

[The use of xenogeneic splenic perfusates for treating suppurative-septic diseases].

It was shown in experiments on a model of fatal staphylococcal sepsis in 200 mice and 15 dogs that the perfusate of a pig's spleen obtained by perfusion of the isolated organ with a dextran solution (rheopolyglucin, polyglucin) is capable of preventing the animals' death. Intravenous infusion of 400-500 ml of a xenospleen perfusate at an interval of 1-3 days, which was included in the complex of postoperative treatment of 45 patients with destructive diseases of the abdominal organs complicated by peritonitis and sepsis, contributed to reduction of lethality and the terms of recovery. It is shown that the main effect, besides detoxification, is based on immunomodulation at the cost of biologically active substances produced by the cells of a xenospleen and contained in the perfusate. The best results were produced with the use of the perfusate of a xenospleen in the early periods after radical operation and thorough cleansing of the source of the infection.

Acute Disease↗
PubMed1991

[Xenogeneic splenic perfusion in the treatment of bronchial asthma patients].

Blood morphology, external respiratory function, central hemodynamics, cellular and humoral immunity, circulating immune complexes, average molecules and leucocytic index were examined in 40 patients with a severe and average severe course of infection-dependent bronchial asthma before and after xenosplenoperfusion. Positive clinical results were noted manifested in an increase of the immune reactivity, improvement of external respiratory function. The method should be used with care due to additional sensibilization of the patient and possible bronchospasm.

Adult↗
PubMed1990

[The treatment of infectious-allergic bronchial asthma by the extracorporeal connection of a donor spleen].

A total of 25 patients suffering from infective allergic bronchial asthma underwent extracorporeal connection of the donor's spleen (ECCDS). Before ECCDS was made the health status was unstable in all the patients, with asphyxia attacks occurring fairly often, namely up to 12 times a day. Nine patients received the maintenance therapy with steroid hormones. After ECCDS 23 persons (92%) manifested complete disappearance of asphyxia attacks or considerable abatement of their severity and rate. In all the steroid-dependent patients, the daily dosage of steroids could be lowered, while in 3 patients, hormonal drugs were afterwards discontinued. Examination of external respiratory function demonstrated the improvement of bronchial patency at the level of large, medium-size and small bronchi. There was a decrease of the concentration of the circulating immune complexes and IgE in the blood plasma. During 2 years of the follow up, 16 patients did not show any disease relapses, whereas the remainder demonstrated less gravity of attacks as compared to the period preceding the use of ECCDS. It is concluded that the use of ECCDS is desirable in the multimodality treatment of infective allergic bronchial asthma.

Adult↗
PubMed1989

[The generation of active microbicidal forms of oxygen by leukocytes transiting the vascular bed of the lungs].

In experiments on dogs using the chemiluminescent method and nitroblue-tetrazole reaction the authors found out that leucocytes while passing through the pulmonary vessels bed, in contrast to the spleen increase the generation of active microbicidal forms of oxygen. Due to this fact we suppose that the lungs may take part in the formation of free radical status and phagocytic antiinfectious defence of the organism.

Animals↗
PubMed1988

[Elimination of immune complex deposits from the kidneys of autoimmune NZB/N mice administered a xeno-spleen perfusate].

54 autoimmune NZB/N mice, 6 and 10 months of age, were intravenously injected 0.2 ml solution prepared during perfusion of the isolated sheep spleen with a buffer solution. Perfusion solution was injected 8 times with 2-3-day intervals. The control group of 43 mice received intravenously 0.2 ml of a buffer solution in the similar manner. After the treatment the levels of anti-DNA antibodies and circulating immune complexes were significantly decreased in the sera of mice which received the perfusion solution, as compared with the levels of control groups. Immunofluorescent studies showed a marked decrease in the number of glomerular immune complexes deposits in mice treated with perfusion solution. Six- and ten-month old mice exhibited a similar effect. The perfusion solution may be capable of eliminating the immune complexes from the blood and kidneys of autoimmune mice.

Animals↗
PubMed1986

[Experience with extracorporeal use of xenospleen in patients with traumatic shock].

The article presents results of using the extracorporal connection with the donor (porcine) spleen (ECCDS) for the treatment of 14 patients with critical mechanical traumas and their complications. An experience with 30 ECCDS-s has shown them to possess a pronounced detoxicating and immunomodulatory effect followed by a positive clinical result. In 6 cases of ECCDS a temporary chill was noted. There were no other complications.

Adolescent↗
PubMed1975

[Supramolecular changes in preserved corneal tissue exposed to alpha-tocopherol].

Kinetics of chemifluorescence of the tissue of the cornea of isolated dog eyes (induced by 1% hydrogen peroxide) was confronted to the results of determination of the ultrastructural changes in the 30th day the eye preservation in a moist chamber at 4 degrees C with and without replacement of the chamber humour of the eye with alpha-tocopherol. The results of investigations pointed to profound changes of free radical processes in the structural components of the corneal cells with changes in the periods and conditions of preservation. It is supposed that these disturbances underlied the trigger mechanisms of the autolysis. Stabilization of the lipoprotein complexes of the cell in preservation is apparently determined by the capacity of antioxidants, particularly of alpha-tocopherol to maintain oxidative processes at a constant level.

Animals↗