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Biomedical subjects

A Atkinson

Publications and source records attributed to A Atkinson.

72 records · Page 4Linked to original sources
PubMedDate not supplied

Whole cell biosensors--electrochemical and optical approaches to ecotoxicity testing.

Two different approaches to the interrogation of prokaryotic and eukaryotic cells have been explored, with the objective of developing biosensor based rapid ecotoxicity test protocols for use in a wide range of applications. Prokaryotic cells and some eukaryotic cell types lend themselves to interrogation by mediated amperometry, a technique that allows metabolic activity of the cell to be monitored by accessing cellular redox events with a chemical mediator. Reduction of the mediator by the cell is followed by re-oxidation at an electrode surface poised at a fixed potential. The resulting current flow is proportional to the metabolic status of the cell. A commercial ecotoxicity test, CellSense, employing such whole cell biosensors has now been developed. An alternative approach to interrogation of vertebrate cells has been the use of luminescent reporter genes to determine changes in the metabolic status of the cells following environmental challenge. Several clones have been established of epithelial cells from the bluegill sunfish (BF-2), transfected with the pCIneoLuc plasmid, that express luciferase constitutively. Protocols using these transformed cells are being developed to provide an alternative to the standard neutral red retention test.

Animals↗
PubMedDate not supplied

Nursing burn wounds on general wards.

In this article, the author predicts that patients with intermediate and minor burn injuries will be increasingly nursed on general wards. In this context, she describes developments in the treatment and management of wounds caused by burns, and examines the advantages and drawbacks of current nursing treatments and how these should be used.

Body Surface Area↗
PubMedDate not supplied

Managing people with dementia: CADE units.

In this, the last article in our series on caring for people with dementia in acute units, we depart from our accustomed format and offer an example of excellence in long-stay care. The author describes the philosophy and functioning of a CADE unit in Australia, which typifies what appropriate care of people with dementia is, and demonstrates how careful planning and thoughtful interventions can create an environment in which residents, family and staff can develop.

Aged↗